Ganglionic and ovarian action of acetylcholine during diestrous II in rats. Neuroendocrine control of the luteal regression

Delsouc, María Belén; Bronzi, Cynthia Daniela; Becerra, Cristina del Rosario Daneri; Bonaventura, Maria Marta; Mohamed, Fabián; Casais, Marilina

doi:10.1016/j.steroids.2019.04.005

Cited by 5 publications

(4 citation statements)

References 49 publications

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“…However, other studies 82,83 that used a similar ex vivo culture system (celiac ganglion-superior ovarian nerve-ovarian system) and DO system reported no difference between controls during diestrus.…”

Section: Discussionmentioning

confidence: 90%

“…This effect could be related to the loss of peripheral innervation because this is the only difference between the groups. However, other studies 82,83 that used a similar ex vivo culture system (celiac ganglion‐superior ovarian nerve‐ovarian system) and DO system reported no difference between controls during diestrus. It is therefore possible that the neural control of ovarian pro/antiapoptotic processes depends on the phase of the cycle, being more important during estrus, when drastic changes in ovarian histoarchitecture occur.…”

Section: Discussionmentioning

confidence: 92%

“…126,127 Indeed, endogenous GABA can inhibit the release of NA in stimulated ovarian sympathetic nerve fibers, 128 and could be responsible for the effects of ALLO. Some of the known effects of NA in the ovary are steroidogenesis modulation, 83,129,130 ovulation, 131 promotion of follicle development 132,133 and corpora lutea function. 63 Alterations in the ovarian noradrenergic modulation thus have important effects in ovarian physiology and might mediate some of the the effects of ALLO.…”

Section: Discussionmentioning

confidence: 99%

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Superior mesenteric ganglion neural modulation of ovarian angiogenesis, apoptosis and proliferation by the neuroactive steroid allopregnanolone

Cáceres

Arboccó

Cardone

et al. 2021

J Neuroendocrinology

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Allopregnanolone (ALLO), a potent neuroactive steroid, is synthesized and active in the peripheral nervous system. Previous studies have shown that ALLO participates in the central regulation of reproduction with effects on ovarian physiology, although there is little evidence for its ability to modulate peripheral tissues. The present study aimed to determine whether ALLO, administered to an ex vivo system that comprises the superior mesenteric ganglion (SMG), the ovarian nervous plexus (ONP) and the ovary (O), or to the denervated ovary (DO), was able to modify ovarian apoptosis, proliferation and angiogenesis. For this purpose, the SMG‐ONP‐O system and DO were incubated during 120 min at 37°C, in the presence of two ALLO doses (0.06 µm and 6 µm). The intrinsic and extrinsic pathways of apoptosis were analyzed. Incubation of the SMG‐ONP‐O system with ALLO 0.06 µm led to an increase in the BAX/BCL‐2 ratio and a reduction of FAS‐L mRNA levels. ALLO 6 µm induced a decrease of FAS‐L levels. Incubation of DO with ALLO 0.06 µm reduced FAS‐L, whereas ALLO 6 µm significantly increased it. Cyclin D1 mRNA was measured to evaluate proliferation. Treatment with ALLO 6 µm increased proliferation in both SMG‐ONP‐O and DO. ALLO 0.06 µm produced an increase of Cyclin D1 in DO only. Administration of either ALLO dose led to a higher ovarian expression of vascular endothelial growth factor in the SMG‐ONP‐O system, but a lower one in the DO system. ALLO 6 µm induced ovarian sensitization to GABA by increasing GABAA receptor expression. In conclusion, ALLO participates in the peripheral neural modulation of ovarian physiology. It can also interact directly with the ovarian tissue, modulating key mechanisms involved in normal and pathological processes in a dose‐dependent manner.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Superior mesenteric ganglion neural modulation of ovarian angiogenesis, apoptosis and proliferation by the neuroactive steroid allopregnanolone

Cáceres

Arboccó

Cardone

et al. 2021

J Neuroendocrinology

Self Cite

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“…It cannot be ruled out that upon intraovarian administration of an M1 blocker, this drug could diffuse away from the ovary and act in the central nervous system or that it acts through a sensory pathway to regulate the LH release from the pituitary by an action at the hypothalamus and hence GnRH secretion (Orozco et al, 2010 ). The administration of ACh to an ex‐vivo superior mesenteric ganglion—ovarian plexus nerve—ovary preparation, decreased progesterone and increased androstenedione in parallel to an increase in NO, suggesting an indirect action of ACh on steroid secretion from the ovary (Delsouc et al, 2019 ). Whether ACh may act, however, locally within the ovary and could regulate steroid production by activating MR of, for example, GCs remained unknown.…”

Section: Discussionmentioning

confidence: 99%

Muscarinic receptors in the rat ovary are involved in follicular development but not in steroid secretion

Cuevas

Bastías

Alanis

et al. 2022

Physiological Reports

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Acetylcholine (ACh) may be involved in the regulation of ovarian functions. A previous systemic study in rats showed that a 4‐week, intrabursal local delivery of the ACh‐esterase blocker Huperzine‐A increased intraovarian ACh levels and changed ovarian follicular development, as evidenced by increased healthy antral follicle numbers and corpora lutea, as well as enhanced fertility. To further characterize the ovarian cholinergic system in the rat, we studied whether innervation may contribute to intraovarian ACh. We explored the cellular distribution of three muscarinic receptors (MRs; M1, M3, and M5), analyzed the involvement of MRs in ovarian steroidogenesis, and examined their roles in ovarian follicular development in normal conditions and in animals exposed to stressful conditions by employing the muscarinic antagonist, atropine. Denervation studies decreased ovarian norepinephrine, but ovarian ACh was not affected, evidencing a local, nonneuronal source of ACh. M1 was located on granulosa cells (GCs), especially in large antral follicles. M5 was associated with the ovarian vascular system and only traces of M3 were found. Ex vivo ovary organo‐typic incubations showed that the MR agonist Carbachol did not modify steroid production or expression of steroid biosynthetic enzymes. Intrabursal, in vivo application of atropine (an MR antagonist) for 4 weeks, however, increased atresia of the secondary follicles. The results support the existence of an intraovarian cholinergic system in the rat ovary, located mainly in follicular GCs, which is not involved in steroid production but rather via MRs exerts trophic functions and regulates follicular atresia.

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Associations of single and multiple organophosphate pesticide exposure with female infertility in the USA: data from the 2015–2018 National Health and Nutrition Examination Survey

Weng

Liu

et al. 2022

Environ Sci Pollut Res

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Ganglionic and ovarian action of acetylcholine during diestrous II in rats. Neuroendocrine control of the luteal regression

Cited by 5 publications

References 49 publications

Superior mesenteric ganglion neural modulation of ovarian angiogenesis, apoptosis and proliferation by the neuroactive steroid allopregnanolone

Superior mesenteric ganglion neural modulation of ovarian angiogenesis, apoptosis and proliferation by the neuroactive steroid allopregnanolone

Muscarinic receptors in the rat ovary are involved in follicular development but not in steroid secretion

Associations of single and multiple organophosphate pesticide exposure with female infertility in the USA: data from the 2015–2018 National Health and Nutrition Examination Survey

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