Ganoderma lucidum polysaccharides protect fibroblasts against UVB-induced photoaging

Abstract

Ganoderma lucidum has featured in traditional Chinese medicine for >1,000 years. Ganoderma polysaccharides (GL-PS), a major active ingredient in Ganoderma, confer immune regulation, antitumor effects and significant antioxidant effects. The aim of the present study was to investigate the efficacy and mechanism of GL-PS-associated inhibition of ultraviolet B (UVB)-induced photoaging in human fibroblasts in vitro. Primary human skin fibroblasts were cultured, and a fibroblast photoaging model was built through exposure to UVB. Cell viability was measured by MTT assay. Aged cells were stained using a senescence-associated β-galactosidase staining (SA-β-gal) kit. ELISA kits were used to analyze matrix metalloproteinase (MMP) −1 and C-telopeptides of Type I collagen (CICP) protein levels in cellular supernatant. ROS levels were quantified by flow cytometry. Cells exposed to UVB had decreased cell viability, increased aged cells, decreased CICP protein expression, increased MMP-1 protein expression, and increased cellular ROS levels compared with non-exposed cells. However, cells exposed to UVB and treated with 10, 20 and 40 µg/ml GL-PS demonstrated increased cell viability, decreased aged cells, increased CICP protein expression, decreased MMP-1 protein expression, and decreased cellular ROS levels compared with UVB exposed/GL-PS untreated cells. These results demonstrate that GL-PS protects fibroblasts against photoaging by eliminating UVB-induced ROS. This finding indicates GL-PS treatment may serve as a novel strategy for antiphotoaging.

A Critical Review on Health Promoting Benefits of Edible Mushrooms through Gut Microbiota

“…The total triterpenes in G. lucidum induce apoptosis in MCF-7 cells and attenuate Dimethylbenz[a]anthracene (DMBA) induced mammary and skin carcinomas in experimental animals [ 14 ]. G. lucidum polysaccharides also protect fibroblasts against UVB-induced photoaging [ 15 ]. G. lucidum also showed strong anti-inflammatory activity and it acted as an immunomodulator in inflammation induced by a high-cholesterol diet [ 16 ].…”

Advances in research on the active constituents and physiological effects of Ganoderma lucidum

“…Modern medical research has shown that G. lucidum contains a variety of compounds with anticancer (Kao et al 2016), hypoglycemic (Yang et al 2018), liver protection (Zhao et al 2019), and anti-inflammatory (Hasnat et al 2015) effects. Studies also suggest that G. lucidum possesses strong antioxidant (Lee et al 2016) antimelanogenesis (Hsu et al 2016), anti-aging (Zeng et al 2017), and skin barrier-repairing (Montalbano 2018) properties. Thus, G. lucidum is important as the lead for the development of pharmaceuticals, nutraceuticals.…”

“…GLPs can inhibit MMP-1 protein expression, promote C-telopeptides of type I collagen protein, and inhibit ROS production in fibroblasts following UVB treatment (Zeng et al 2017).…”

Evaluating the effect of Ganoderma lucidum polysaccharides on five cytochrome P450 isozymes with cocktail probe drugs in rats by LC–MS/MS

“…Ganoderma lucidum (GL), as a famous herbal medicine in China and Japan more than 2000 years, has been prized for its medicinal vitality such as promoting longevity and benefiting for general health [1,2]. Especially, GL polysaccharides (GLPs) are a major bioactive ingredient and has been identified that it has regulatory abilities in most of physiological progress, including antitumor effects, immune modulation, significant antioxidant activity, and hypoglycaemic [3][4][5][6][7][8].…”