Gap Junctions in Mesangial and Lacis Cells

Pricam, C; Humbert, F; Perrelet, Alain; Orci, Lelio

doi:10.1083/jcb.63.1.349

Cited by 92 publications

(28 citation statements)

References 16 publications

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“…(2) MC also reside in the GJ interstitium, surrounded by afferent and efferent arterioles and the macula densa. Morphological documentation and histochemical studies have revealed the presence of abundant GJ among intraglomerular and extraglomerular MC and afferent arteriolar cells, and this structure makes these cells very tightly coupled and unites them as a syncytium (1,2,7,8). The functional data on transmission of Ca 2ϩ signaling via GJ in this study provides important evidence, supporting the idea of a mediating role of extraglomerular MC in TG-feedback mechanisms.…”

Section: Discussionsupporting

confidence: 65%

“…Additionally, the presence of gap junctional intercellular communication (GJIC) among cultured rat MC, its regulation by pathophysiologic factors, and its potential role in the propagation of Ca 2ϩ waves have also been reported (6,7). It has been speculated that GJ, acting as a sophisticated cellular communication system, bridges each MC between the juxtaglomerular region (extraglomerular mesangium) and the glomerular mesangium and provides the mesangium with the characteristics of a functional syncytium (1,7,8).…”

mentioning

confidence: 99%

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Coordination of Mesangial Cell Contraction by Gap Junction–Mediated Intercellular Ca2+ Wave

Yao

Morioka

et al. 2002

Journal of the American Society of Nephrology

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Abstract. Gap junction intercellular communication (GJIC) plays a fundamental role in mediating intercellular signals and coordinating multicellular behavior in various tissues and organs. Glomerular mesangial cells (MC) are rich in GJ, but the functional associations of these intercellular channels are still unclear. This study examines the potential role of GJ in the transmission of intercellular Ca 2ϩ signals and in the coordination of MC contraction. First, the presence of GJ protein Cx43 and functional GJIC was confirmed in MC by using immunochemical staining or transfer of Lucifer yellow (LY) after a single cell injection, respectively. Second, mechanical stimulation of a single MC initiated propagation of an intercellular Ca 2ϩ wave, which was preventable by the GJ inhibitor heptanol but was not altered by pretreatment of MC with ATP or addition of apyrase into the assay system. Third, the phospholipase C (PLC) inhibitor U73122 could largely eliminate the mechanically elicited propagation of intercellular Ca 2ϩ waves, suggesting a possibly mediating role of inositol trisphosphate (IP 3 ) in the initiation and transmission of intercellular Ca 2ϩ signaling. Fourth, injection of IP 3 into a single cell caused contraction, not only in the targeted cell, but also in the adjacent cells, as indicated by the reduction of cellular planar area. Fifth, addition of two structurally unrelated GJ inhibitors, heptanol and ␣-glycyrrhetinic acid (GA), into MC embedded in collagen gels significantly attenuated the reduction of gel areas after exposure to serum. This study provides the first functional evidence supporting the critical role of GJIC in the synchronization of MC behaviors.

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Section: Discussionsupporting

confidence: 65%

mentioning

confidence: 99%

Coordination of Mesangial Cell Contraction by Gap Junction–Mediated Intercellular Ca2+ Wave

Yao

Morioka

et al. 2002

Journal of the American Society of Nephrology

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“…One of the striking features of MC is that these cells possess extremely high density of GJ (20,21). This unique property indicates a possibility that MC are actively involved in the function of the juxtaglomerular apparatus (22).…”

mentioning

confidence: 96%

Nitric Oxide-Mediated Regulation of Connexin43 Expression and Gap Junctional Intercellular Communication in Mesangial Cells

Yao¹,

Hiramatsu²,

Zhu

et al. 2005

Journal of the American Society of Nephrology

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“…This lack of specialised junctions was one reason to suspect chemical communication between these two cell types. Mesangial cells are modified smooth muscle cells that, in part, play a structural role supporting the glomerular capillary bed and are also in contact with the afferent and efferent arterioles [8][9][10]. These cells do exhibit gap junctions and connexins and there does appear to be communication between mesangial cells and also between mesangial cells and arteriolar segments and other elements within the JGA.…”

Section: Introductionmentioning

confidence: 99%

ATP as a mediator of macula densa cell signalling

Bell

Komlósi

Zhang

2009

Purinergic Signalling

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Within each nephro-vascular unit, the tubule returns to the vicinity of its own glomerulus. At this site, there are specialised tubular cells, the macula densa cells, which sense changes in tubular fluid composition and transmit information to the glomerular arterioles resulting in alterations in glomerular filtration rate and blood flow. Work over the last few years has characterised the mechanisms that lead to the detection of changes in luminal sodium chloride and osmolality by the macula densa cells. These cells are true "sensor cells" since intracellular ion concentrations and membrane potential reflect the level of luminal sodium chloride concentration. An unresolved question has been the nature of the signalling molecule(s) released by the macula densa cells. Currently, there is evidence that macula densa cells produce nitric oxide via neuronal nitric oxide synthase (nNOS) and prostaglandin E 2 (PGE 2 ) through cyclooxygenase 2 (COX 2)-microsomal prostaglandin E synthase (mPGES). However, both of these signalling molecules play a role in modulating or regulating the macula-tubuloglomerular feedback system. Direct macula densa signalling appears to involve the release of ATP across the basolateral membrane through a maxi-anion channel in response to an increase in luminal sodium chloride concentration. ATP that is released by macula densa cells may directly activate P2 receptors on adjacent mesangial cells and afferent arteriolar smooth muscle cells, or the ATP may be converted to adenosine. However, the critical step in signalling would appear to be the regulated release of ATP across the basolateral membrane of macula densa cells.

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Gap Junctions in Mesangial and Lacis Cells

Cited by 92 publications

References 16 publications

Coordination of Mesangial Cell Contraction by Gap Junction–Mediated Intercellular Ca2+ Wave

Coordination of Mesangial Cell Contraction by Gap Junction–Mediated Intercellular Ca2+ Wave

Nitric Oxide-Mediated Regulation of Connexin43 Expression and Gap Junctional Intercellular Communication in Mesangial Cells

ATP as a mediator of macula densa cell signalling

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