Gardnerella vaginalis triggers NLRP3 inflammasome recruitment in THP-1 monocytes

Vick, Eric; Park, Hyo‐Sang; Huff, Krista A.; Brooks, Kenneth M.; Farone, Anthony L.; Farone, Mary B.

doi:10.1016/j.jri.2014.08.005

Cited by 29 publications

(32 citation statements)

References 39 publications

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“…A number of studies have also shown that the proliferation of Gardnerella vaginalis associated with inflammatory response can be inhibited by Lactobacillus. By activating TLR-2 on the surface of monocytic THP-1 cells, G. vaginalis activates NF-kB to induce the secretion of large amounts of TNF-α [19,20]. Similar increases in TNF-α levels have also been reported from a study on a transgenic rat model of POF [21].…”

Section: Introductionsupporting

confidence: 67%

Changes in the vaginal microbiota associated with primary ovarian failure

Wang

Han

et al. 2020

Preprint

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Background: Primary ovary failure (POF) is defined as follicular failure in women of reproductive age. Although many factors are speculated to contribute to the occurrence of POF, the exact aetiology is unclear. Alterations in the microbiome of women with POF are poorly studied; Methods: This study investigated the vaginal microbiota of 22 patients with POF and 29 healthy individuals. High-throughput Illumina Miseq sequencing targeting the V3-V4 region of the 16S ribosomal RNA (rRNA) gene was used to evaluate the relationships between vaginal flora and clinical characteristics of POF. Results: Different from before, we found that the diversity and richness of the vaginal flora of patients with POF was significantly different from that of healthy controls. Comparison of the flora of patients with POF with that of menopausal women revealed that the relative abundance of Lactobacillus was significantly reduced in the latter, and women with reduced relative abundance of Lactobacillus in microbiota community decreased the pregnancy success rate at term. The result suggests the present study enabled identification of microbiota associated with POF, further investigations of differences in the microbiota in the context of POF will improve our understanding of the pathogenesis of the disease which involve modification of the vaginal microbiota. Conclusion: The present study identified the microbiota associated with POF. Further investigations on the differences in the microbiota in the context of POF will improve our understanding of the pathogenesis of the disease which involves modification of the vaginal microbiota.

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Section: Introductionsupporting

confidence: 67%

Changes in the vaginal microbiota associated with primary ovarian failure

Wang

Han

et al. 2020

Preprint

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“…This sialic acid is used by pathogens as a mechanism of adherence to cellular and inert surfaces, as a source of nutrition and also modifies the normal mucus barrier and immune response (Amith et al 2009, 2010; Stafford et al 2011; Lewis et al 2012, 2013; Vick et al 2014). Evidence suggests BV in conjunction with high sialidase activity is predictive of PTB and low birthweight, while BV alone is not (Cauci et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Inhibition of sialidase activity and cellular invasion by the bacterial vaginosis pathogen Gardnerella vaginalis

et al. 2018

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Bacterial vaginosis is a genital tract infection, thought to be caused by transformation of a lactobacillus-rich flora to a dysbiotic microbiota enriched in mixed anaerobes. The most prominent of these is Gardnerella vaginalis (GV), an anaerobic pathogen that produces sialidase enzyme to cleave terminal sialic acid residues from human glycans. Notably, high sialidase activity is associated with preterm birth and low birthweight. We explored the potential of the sialidase inhibitor Zanamavir against GV whole cell sialidase activity using methyl–umbelliferyl neuraminic acid (MU-NANA) cleavage assays, with Zanamavir causing a 30% reduction in whole cell GV sialidase activity (p < 0.05). Furthermore, cellular invasion assays using HeLa cervical epithelial cells, infected with GV, demonstrated that Zanamivir elicited a 50% reduction in cell association and invasion (p < 0.05). Our data thus highlight that pharmacological sialidase inhibitors are able to modify BV-associated sialidase activity and influence host–pathogen interactions and may represent novel therapeutic adjuncts.

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“…При воспалительных заболеваниях вульвы и влагалища также отмечается повышение синтеза ФНО-альфа и ИЛ-1, в т. ч. и у беременных пациенток [9][10][11]. Повы шенный цитокиновый фон, высокая активность G. vagi nalis на фоне дополнительной супрессии нормальной микрофлоры влагалища антисептиками могут провоцировать быструю гибель нормальных клеток слизистой в зоне воспаления в результате процесса пироптоза [12,13].…”

Section: патогенетическое обоснование применения бензидамина при острunclassified

Mechanisms of benzydamine action against local inflammatory process

2019

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Local inflammatory diseases caused by various infections are one of the most common pathologies in medical practice. For example, tonsillopharyngitis. This disease is extremely frequent for a physician’s practice. There is a fair amount of drugs, which supposed to be helpful against tonsillopharyngitis, but different drugs are also not the same in their pharmacological effects. In gynecological practice, frequent diseases are specific and non-specific vulvovaginitis, which have inflammatory and infectious components. For administrating local pharmacotherapy in gynecology against inflammation, a large number of drugs with different mechanisms of action are proposed. In this study, we focused on key pathological mechanisms associated with acute and chronic inflammation, for which these drugs should be exposed by their pharmacokinetic and pharmacodynamic properties. The best combination of these properties is available for benzydamine hydrochloride. Benzydamine hydrochloride has a wide antimicrobic activity against bacteria and Candida spices both albicans and non-albicans strains and allows to influence on etiologic cause of the disease. Also benzydamine hydrochloride associated with «cytokine» mechanism of anti-inflammatory action, which means that he does not affect COX enzymes and it allows to avoid gastrotoxic adverse events. Also, in this work showed and discussed aspects of the interaction of benzydamine with local immune system and justification of useful usage the benzydamine for local therapy of acute and chronic inflammatory processes caused by various infections.

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Gardnerella vaginalis triggers NLRP3 inflammasome recruitment in THP-1 monocytes

Cited by 29 publications

References 39 publications

Changes in the vaginal microbiota associated with primary ovarian failure

Changes in the vaginal microbiota associated with primary ovarian failure

Inhibition of sialidase activity and cellular invasion by the bacterial vaginosis pathogen Gardnerella vaginalis

Mechanisms of benzydamine action against local inflammatory process

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