Garenoxacin-induced increase of CD11b expression on human polymorphonuclear neutrophils does not affect phagocytosis and killing of Staphylococcus aureus

Grüger, Thomas; Reiners, Ana-Lena; Brandenburg, Kerstin; Zündorf, Josef

doi:10.1007/s10156-010-0099-8

Cited by 2 publications

(3 citation statements)

References 16 publications

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“…A combination of dextran (2%) sedimentation and subsequent Percoll centrifugation (66% and 76% Percoll gradients) was used for isolation of PMNs from heparinized blood. The staining procedure, cell fixation and flow cytometric analysis were performed as described previously [16].…”

Section: Methodsmentioning

confidence: 99%

“…Initially and after 2 h of rotation, the suspension of PMNs and bacteria was plated in serial dilutions (10 −2 to 10 −4 ) onto HHD. The analysis was performed as described previously [16].…”

Section: Methodsmentioning

confidence: 99%

“…The bacteria from overnight culture (stationary phase) were washed twice and incubated with human fibrinogen (1 mg/mL; Sigma-Aldrich, St Louis, MO, USA) for 10 min at 37°C. The bacteria were then washed again and stained with an anti-human fibrinogen antibody (Agrisera, Vaennaes, Sweden; Lot 0911) and analysed by fluorescence-activated cell sorting as described previously [16].…”

Section: Cell Aggregation Studies and Fibrinogen-binding Assaymentioning

confidence: 99%

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Phenotyping of Staphylococcus aureus reveals a new virulent ST398 lineage

Schmidt

Zündorf

Grüger

et al. 2013

Clinical Microbiology and Infection

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Staphylococcus aureus sequence type (ST)398, which is commonly found as a colonization strain in pig farming, is emerging more frequently as the cause of human infections. In this study, we analysed ST398 of porcine and human origin at the genetic, protein and immunogenic levels. Although genetic analysis of the genes encoding the major virulence factors revealed the presence of the same genes in all strains studied, the results demonstrated spa type crossing alterations in adhesion abilities in addition to a strongly enhanced lysis activity directly linked to impaired clearance attributable to polymorphonuclear leukocytes (PMNs). This change in virulence pattern indicates high heterogenicity in the ST398 pool that is not based on a different genetic make-up, but probably on variations in the genetic regulation systems. These modifications, which are tightly connected to pathogenicity, cannot be detected by conventional diagnostic methods.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Cell Aggregation Studies and Fibrinogen-binding Assaymentioning

confidence: 99%

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Phenotyping of Staphylococcus aureus reveals a new virulent ST398 lineage

Schmidt

Zündorf

Grüger

et al. 2013

Clinical Microbiology and Infection

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CD66b overexpression and homotypic aggregation of human peripheral blood neutrophils after activation by a gram-positive stimulus

Schmidt

Zündorf

Grüger

et al. 2012

Journal of Leukocyte Biology

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Neutrophils represent the main component of innate immunity in the clearance of bacterial infections. To pass the tissue and to localize and reach the site of infection, the peripheral blood neutrophils have to pass through a complex receptor-mediated interaction with the endothelial layer. Under pathophysiological conditions, such as severe sepsis, this process is impaired and often characterized by neutrophil aggregation. In this study, we examined the impact of three different Staphylococcus aureus strains on the activation status of human peripheral blood neutrophils by coincubation of bacterial culture supernatant with whole blood. This complex interaction of a gram-positive stimulus with blood components leads to a special neutrophil activation phenotype, which is characterized by an overexpression of the cell-surface molecule CD66b. The process is accompanied by a strong increase of homotypic aggregates and seems to be initialized by a massive activation impulse caused by the interplay of plasma components. This maximum activation of neutrophils prior to the complex and highly regulated activation required for transmigration might play a key role in the neutrophil dysfunction in gram-positive sepsis.

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Garenoxacin-induced increase of CD11b expression on human polymorphonuclear neutrophils does not affect phagocytosis and killing of Staphylococcus aureus

Cited by 2 publications

References 16 publications

Phenotyping of Staphylococcus aureus reveals a new virulent ST398 lineage

Phenotyping of Staphylococcus aureus reveals a new virulent ST398 lineage

CD66b overexpression and homotypic aggregation of human peripheral blood neutrophils after activation by a gram-positive stimulus

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