GART expression in rat spinal cord after injury and its role in inflammation

Zhang, Dongmei; Yue, Ying; Jiang, Shengyang; Li, Aihong; A, Guo; Wu, Xinming; Xia, Xiaopeng; Cheng, Hongbing; Zhang, Jinlong; Tao, Tao; Gu, Xingxing

doi:10.1016/j.brainres.2014.03.044

Cited by 11 publications

(8 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Extracellular purines can activate astrocytes and microglial cells in response to CNS injury or neurodegeneration and increased levels of purine nucleotides may promote astrocytic hypertrophy 25 . Zhang et al (2014) 26 reported that GART is mainly localized in astrocytes and its expression is enhanced after CNS injury and neuroinflammation, which eventually causes astrocyte activation and neural apoptosis. Activated astrocytes release many different types of potentially neurotoxic and proinflammatory mediators, including cytokines and growth factors such as IFNs, IL-1β, IL-6, TNF-α, innate immunity mediators such as LPS and Toll-like receptor (TLR) ligands, neurotransmitters such as glutamate and noradrenalin, purine nucleotides such as adenosine triphosphate (ATP), and reactive oxygen species (ROS) such as NO 27 .…”

Section: Discussionmentioning

confidence: 99%

“…Activated astrocytes release many different types of potentially neurotoxic and proinflammatory mediators, including cytokines and growth factors such as IFNs, IL-1β, IL-6, TNF-α, innate immunity mediators such as LPS and Toll-like receptor (TLR) ligands, neurotransmitters such as glutamate and noradrenalin, purine nucleotides such as adenosine triphosphate (ATP), and reactive oxygen species (ROS) such as NO 27 . Thus, GART might be involved in the activation of astrocytes and regulate proinflammatory mediator-related immunological reactions 26 . Although poly I:C-treated mice did not show the changes in mRNA level of Gart in both PFC and HIP (Table 4), the most promising molecules might be involved in the pathophysiology of SCZ.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Proteomic analysis of lymphoblastoid cell lines from schizophrenic patients

et al. 2019

View full text Add to dashboard Cite

Although a number of studies have identified several convincing candidate genes or molecules, the pathophysiology of schizophrenia (SCZ) has not been completely elucidated. Therapeutic optimization based on pathophysiology should be performed as early as possible to improve functional outcomes and prognosis; to detect useful biomarkers for SCZ, which reflect pathophysiology and can be utilized for timely diagnosis and effective therapy. To explore biomarkers for SCZ, we employed fluorescence two-dimensional differential gel electrophoresis (2D-DIGE) of lymphoblastoid cell lines (LCLs) [1st sample set: 30 SCZ and 30 control subjects (CON)]. Differentially expressed protein spots were sequenced by liquid chromatography tandem-mass spectrometry (LC-MS/MS) and identified proteins were confirmed by western blotting (WB) (1st and 2nd sample set: 60 SCZ and 60 CON). Multivariate logistic regression analysis was performed to identify an optimal combination of biomarkers to create a prediction model for SCZ. Twenty protein spots were differentially expressed between SCZ and CON in 2D-DIGE analysis and 22 unique proteins were identified by LC-MS/MS. Differential expression of eight of 22 proteins was confirmed by WB. Among the eight candidate proteins (HSPA4L, MX1, GLRX3, UROD, MAPRE1, TBCB, IGHM, and GART), we successfully constructed logistic regression models comprised of 4- and 6-markers with good discriminative ability between SCZ and CON. In both WB and gene expression analysis of LCL, MX1 showed reproducibly significant associations. Moreover, Mx1 and its related proinflamatory genes ( Mx2 , Il1b , and Tnf ) were also up-regulated in poly I:C-treated mice. Differentially expressed proteins might be associated with molecular pathophysiology of SCZ, including dysregulation of immunological reactions and potentially provide diagnostic and prognostic biomarkers.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Proteomic analysis of lymphoblastoid cell lines from schizophrenic patients

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Secondary injury initially stimulates a large number of inflammatory cells, which produce a strong inflammatory reaction and eventually glial cell death (10). Inflammation factors is a major neurotransmitter regulating immune response, it is the Bridges of our immune cells and other cells (10). NF-κB p65 and TNF-α are secreted by activated microglia and macrophages, neurons and glial cells during the early stages of TSCI (11).…”

Section: Discussionmentioning

confidence: 99%

Schisandrin B attenuates the inflammatory response, oxidative stress and apoptosis induced by traumatic spinal cord injury via inhibition of p53 signaling in adult rats

Xin

Huo

et al. 2017

Molecular Medicine Reports

View full text Add to dashboard Cite

Schisandrin B is an active monomer of the Chinese magnolia vine (Schisandra chinensis) that can reduce transaminase activity in liver cells, inhibit lipid peroxidation, enhance antioxidant status, has protective effects in the liver and has antitumor effects. The present study investigated the potential protective effects of schisandrin B on the p53 signaling pathway in attenuating the inflammatory response, oxidative stress and apoptosis induced by traumatic spinal cord injury (TSCI) in adult rats. Behavioral examination, inclined plate test and spinal cord water content were used to evaluate the protective effect of schisandrin B in TSCI rats. The expression levels of superoxide dismutase (SOD), malondialdehyde (MDA), nuclear factor (NF)-κB subunit p65 and tumor necrosis factor (TNF)-α were examined using ELISA kits. Western blot analysis was performed to analyze the protein expression of caspase-3 and phosphorylated (p)-p53 in TSCI rats. In the present study, schisandrin B improved behavioral examination results and the maximum angle of inclined plate test, and inhibited spinal cord water content in rats with TSCI. Notably, schisandrin B reduced the activation of traumatic injury-associated pathways, including SOD, MDA, NF-κB p65 and TNF-α, in TSCI rats. In addition, schisandrin B suppressed the TSCI-induced expression of caspase-3 and p-p53 in TSCI rats. These results indicated that schisandrin B may attenuate the inflammatory response, oxidative stress and apoptosis in TSCI rats by inhibiting the p53 signaling pathway in adult rats.

show abstract

“…The glycinamide ribonucleotide transformylase (GART) gene coding a functional polypeptide was required for purine biosynthesis. Zhang et al () demonstrated that GART might be important in spinal cord injury. Protein phosphatase (PP) type 2A was associated with cardiac excitation‐contraction coupling for the multifunctional serine/threonine phosphatase activity.…”

Section: Discussionmentioning

confidence: 99%

Co‐expression analysis provides important module and pathways of human dilated cardiomyopathy

Xiao

Yang

et al. 2019

Journal Cellular Physiology

View full text Add to dashboard Cite

Dilated cardiomyopathy (DCM) is a heart disease that injured greatly to the people wordwide. Systemic co-expression analysis for this cancer is still limited, although massive clinic experiments and gene profiling analyses had been well performed previously. Here, using the public RNA-Seq data "GSE116250" and gene annotation of Ensembl database, we built the co-expression modules for DCM by Weighted Gene Co-Expression Network Analysis, and investigated the function enrichment and protein-protein interaction (PPI) network of co-expression genes of each module by Database for Annotation, Visualization, and Integrated Discovery and Search Tool for the Retrieval of Interacting Genes/Proteins database, respectively. First, 5,000 genes in the 37 samples were screened and 11 co-expression modules were conducted. The number of genes for each module ranged from 77 to 936, with a mean of 455. Second, interaction relationships of hub-genes between pairwise modules showed great differences, suggesting relatively high-scale independence of the modules. Third, functional enrichments of the co-expression modules exhibited great differences. We found that genes in module 3 were significantly enriched in the pathways of focal adhesion and ubiquitin-mediated proteolysis. This module was inferred as the key module involved in DCM. In addition, PPI analysis revealed that the genes HSP90AA1, CTNNB1, MAPK1, GART, and PPP2CA owned the largest number of adjacency genes, unveiling that they may function importantly during the occurrence of DCM. Focal adhesion and ubiquitin-mediated proteolysis play important roles in human DCM.

show abstract

GART expression in rat spinal cord after injury and its role in inflammation

Cited by 11 publications

References 47 publications

Proteomic analysis of lymphoblastoid cell lines from schizophrenic patients

Proteomic analysis of lymphoblastoid cell lines from schizophrenic patients

Schisandrin B attenuates the inflammatory response, oxidative stress and apoptosis induced by traumatic spinal cord injury via inhibition of p53 signaling in adult rats

Co‐expression analysis provides important module and pathways of human dilated cardiomyopathy

Contact Info

Product

Resources

About