Gas-assisted magnetic separation for the purification of proteins in batch systems

Liu, Zhini; Yang, Liangrong; Dong, Tingting; Li, Wensong; Sun, Xitong; Zhu, Menghao; Duan, Zhengkang; Liu, Qingfen; Liu, Huizhou

doi:10.1016/j.partic.2014.12.015

Cited by 5 publications

(1 citation statement)

References 28 publications

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“…Magnetic flotation can be used to collect or to enhance a flotation effect and separate and capture target molecules from impurities efficiently. The gas-assisted magnetic separation (GAMS) and the gas-assisted superparamagnetic extraction (GASE) process use nitrogen bubbles to float magnetic nanoparticles which are bound to target molecules (Li et al, 2013; Liu et al, 2016). The magnetic particles can be collected either in an extraction phase or with a magnet which allows for a fast separation process.…”

Section: Separation Strategy and Separator Designmentioning

confidence: 99%

Magnetic Separation in Bioprocessing Beyond the Analytical Scale: From Biotechnology to the Food Industry

Schwaminger

Fraga-García

Eigenfeld

et al. 2019

Front. Bioeng. Biotechnol.

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Downstream processing needs more innovative ideas to advance and overcome current bioprocessing challenges. Chromatography is by far the most prevalent technique used by a conservative industrial sector. Chromatography has many advantages but also often represents the most expensive step in a pharmaceutical production process. Therefore, alternative methods as well as further processing strategies are urgently needed. One promising candidate for new developments on a large scale is magnetic separation, which enables the fast and direct capture of target molecules in fermentation broths. There has been a small revolution in this area in the last 10–20 years and a few papers dealing with the use of magnetic separation in bioprocessing examples beyond the analytical scale have been published. Since each target material is purified with a different magnetic separation approach, the comparison of processes is not trivial but would help to understand and improve magnetic separation and thus making it attractive for the technical scale. To address this issue, we report on the latest achievements in magnetic separation technology and offer an overview of the progress of the capture and separation of biomolecules derived from biotechnology and food technology. Magnetic separation has great potential for high-throughput downstream processing in applied life sciences. At the same time, two major challenges need to be overcome: (1) the development of a platform for suitable and flexible separation devices and (2) additional investigations of advantageous processing conditions, especially during recovery. Concentration and purification factors need to be improved to pave the way for the broader use of magnetic applications. The innovative combination of magnetic gradients and multipurpose separations will set new magnetic-based trends for large scale downstream processing.

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Section: Separation Strategy and Separator Designmentioning

confidence: 99%

Magnetic Separation in Bioprocessing Beyond the Analytical Scale: From Biotechnology to the Food Industry

Schwaminger

Fraga-García

Eigenfeld

et al. 2019

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

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Superior lithium adsorption and required magnetic separation behavior of iron-doped lithium ion-sieves

Wang

Zheng

Wang

et al. 2018

Chemical Engineering Journal

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A review of magnetic separation of whey proteins and potential application to whey proteins recovery, isolation and utilization

Nicolás

Ferreira

Lassalle

2019

Journal of Food Engineering

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Cheese whey (CW) is a voluminous effluent generating environmental and economic impact in milk producing countries. Proteins from CW are useful for biotechnological applications. Available procedures to purify CW are complex and expensive. Magnetic nanotechnology emerges as an alternative to attain this goal. Magnetic nanoparticles are easily and economically prepared and can be formulated to selectively bind proteins in whey. Magnetic decantation allows simple and fast protein isolation by means of a magnet. The extra advantage is the possibility to regenerate and reuse the magnetic material in successive cycles. In this contribution, competitiveness of magnetic nanodevices is reviewed as a potential tool for the valorization and remediation of milk industry wastes. A critical analysis of recompiled data is included comparing magnetic nanomaterials with the current technologies intended for CW treatments. The purpose is to determine the most important factors that carry towards an effective recovery of proteins for diverse applications.

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Gas-assisted magnetic separation for the purification of proteins in batch systems

Cited by 5 publications

References 28 publications

Magnetic Separation in Bioprocessing Beyond the Analytical Scale: From Biotechnology to the Food Industry

Magnetic Separation in Bioprocessing Beyond the Analytical Scale: From Biotechnology to the Food Industry

Superior lithium adsorption and required magnetic separation behavior of iron-doped lithium ion-sieves

A review of magnetic separation of whey proteins and potential application to whey proteins recovery, isolation and utilization

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