Gas-chromatographic analysis of mycolic acid cleavage products in mycobacteria

Guerrant, G O; Lambert, M A; Moss, C W

doi:10.1128/jcm.13.5.899-907.1981

Cited by 57 publications

(25 citation statements)

References 20 publications

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“…Because of the abundance of mycolic acids in mycobacterial species, several studies have addressed the separation of compounds of both types, i.e., those with chains of >20 carbons (mycolic acid cleavage products) and those with chains of <20 carbons (constitutive fatty acids) (58,87,150). Quantitative analysis of fatty acids belonging to these two groups results in accurate identification to the species level (58,69,82,150,151). Among isolates encountered over a 2-year period, Jantzen et al 69determined that M. tuberculosis could be identified without exception, largely on the basis of C26:0 at concentrations of 1 to 13%.…”

Section: Identification To Genus or Speciesmentioning

confidence: 99%

Applications of cellular fatty acid analysis.

Welch

1991

Clinical Microbiology Reviews

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Section: Identification To Genus or Speciesmentioning

confidence: 99%

Applications of cellular fatty acid analysis.

Welch

1991

Clinical Microbiology Reviews

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“…Mycolic acid cleavage products. The two commonly occumng constituents tetracosanoate (24:O) and hexacosanoate (26:O) of Table 1 are pyrolytic cleavage products of mycolic acids (6,7,14). In agreement with several reports (9,13,14,17,28) we found the amounts of these two products to be reasonably reproducible (Table 1 ), although Lambert et al reported otherwise (10).…”

Section: Main Dj Ffermtiating Jeaturesmentioning

confidence: 99%

Gas chromatography of mycobacterial fatty acids and alcohols: Diagnostic applications

Jantzen

Tangen

Eng

1989

APMIS

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Jantzen, E., Tangen, T. & Eng. J. Gas chromatography of mycobacterial fatty acids and alcohols: Diagnostic applications. APMIS 5'7: [1037][1038][1039][1040][1041][1042][1043][1044][1045] 1989.Capillary gas chromatography of cellular fatty acids and alcohols has been used as a routine method for a period of two years in the mycobacterial diagnostic laboratory of Statens institutt for folkehelse, Oslo, Norway. All mycobacteria (165 isolates) other than Mycobacterium tuberculosis (MOTT) and 24 randomly selected M . tzib~~rczilosis isolates were studied. Twelve characteristic lipid constituents allowed the construction of a diagnostic scheme. Without exceptions. all 36 examined isolates belonging to the M. ttthc,r~.zf,uk)sis-complex were characterized by a relatively high concentration level of hexacosanoic acid (mean: 4%. range: I-l3%), low level of tetracosanoic acid (mean: 1%. range: 0.1-3%), lack of methylbranched acids other than tuberculostearic acid, and lack of fatty alcohols. Members of the MAIS-complex (73 isolates) were all characterized by the general presence of the fatty alcohols 2-octadecanol (mean: 2%, range: 0.1-5%) and 2-eicosanol (mean: 7%, range: 2-2 1 %), relatively high levels of tetracosanoic acid (mean: 5%, range: 1-15%) and lack (or trace) of hexacosanoic acid and methylbranched acids other than tuberculostearic acid. All 16 isolates of M . gordonae were easily recognized by their unique lack of tuberculostearic acid and their content of 2-methyl-tetradecanoic acid (mean: 5%, range: 2-1 2%), and the M . xenopi isolates were the only examined strains containing the fatty alcohol 2-docosanol (mean: 9%, range: 2-13%). The six M . mulmoense strains contained the two unique constituents 2-methyl eicosanoic acid (mean: 39/0, range: 1-4%) and 2,4,6-trimethyl tetracosanoic acid (mean: 3%, range: 2-4%). The ten strains of M . kunsusii were characterized by 2.4-dimethyl tetradecanoic acid (mean: 5%, range: 1-1 I%), whereas the seven strains of M . rriarinum shared 2,4-dimethyl hexadecanoic acid (mean: 4%, range 0.2-12%) as a specific marker.

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“…A variety of GLC methods have been developed in an attempt to differentiate mycobacteria by identifying their complex mycolic acids. Guerrant et al (8) used GLC to examine mycolic acid methyl ester cleavage products of several mycobacterial species and showed the potential of this system for differentiating mycobacteria; however, because of the limited number of species tested and the complexity of the methyl mycolates, the methodology had limited practical application. In recent years, GLC profiles of mycobacterial fatty acid methyl esters has appeared more useful for speciating mycobacteria (1,3,12,21,22).…”

mentioning

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Characterization of Mycobacterium paratuberculosis by gas-liquid and thin-layer chromatography and rapid demonstration of mycobactin dependence using radiometric methods

1987

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Thirty-six Mycobacterium paratuberculosis isolates of bovine, caprine, and ovine origins were evaluated by using gas-liquid chromatography (GLC), thin-layer chromatography (TLC), and BACTEC 7H12 Middlebrook TB medium (12A; Johnston Laboratories, Inc., Towson, Md.) in an effort to more rapidly differentiate this group of organisms from other mycobacteria. Bacterial suspensions (0.1 ml) were inoculated by syringe into 7H12 broth containing 2 ,ug of mycobactin P per ml and control broth without mycobactin P. Cultures were incubated at 37°C and read daily with a BACTEC Model 301 (Johnston Laboratories). After 8 days of incubation, the growth index readings for the test broths containing mycobactin P were twice those of the control broths without mycobactin P. Sixty-five isolates of mycobacteria other than M. paratuberculosis were also examined. No difference was noted between the growth index readings of control and mycobactincontaining broths. Except for Mycobacterium avium-Mycobacterium intracellulare, TLC studies differentiated M. paratuberculosis from the other mycobacterial species tested. The GLC data reveal that àll M. paratuberculosis isolates had a distinctive peak (14A) which was not found among M. avium-M. intracellulare complex organisms. These data indicate that 7H12 radiometric broth was able to rapidly demonstrate the mycobactin dependence of M. paratuberculosis and GLC and TLC procedures were capable of rapidly differentiating this organism from the other mycobacteria studied.

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Gas-chromatographic analysis of mycolic acid cleavage products in mycobacteria

Cited by 57 publications

References 20 publications

Applications of cellular fatty acid analysis.

Applications of cellular fatty acid analysis.

Gas chromatography of mycobacterial fatty acids and alcohols: Diagnostic applications

Characterization of Mycobacterium paratuberculosis by gas-liquid and thin-layer chromatography and rapid demonstration of mycobactin dependence using radiometric methods

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