Gas Chromatographic Analysis of the Fatty Acid Composition of the Plasma Lipids in Normal and Diabetic Subjects*

Hallgren, Bo; Stenhagen, Stina; Svanborg, Alvar; Svennerholm, Lars

doi:10.1172/jci104162

Cited by 152 publications

(17 citation statements)

References 18 publications

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“…Values obtained in the present experiments were similar to those on Japanese subjects by OYAMA et al .14) and on the white by several investigators13, [15][16][17][18][19][20] , though higher average values amounting to 138 and 152mg/100ml were also reported by SCHWARTS et al . 21) and DUNN and MOSES22) respectively.…”

Section: Triglyceridessupporting

confidence: 93%

Plasma Lipid Profiles in the Winter of Hokkaido Inhabitants

et al. 1969

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Section: Triglyceridessupporting

confidence: 93%

Plasma Lipid Profiles in the Winter of Hokkaido Inhabitants

et al. 1969

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“…On the other hand, the lipids of the lipoproteins in normal fasting plasma remain unaltered after a single meal, and approach the fatty acid composition of the dietary fat only after continuous feeding for several weeks or longer (31,32). In this study, we have demonstrated that in fasting plasma the changes in esterified fatty acids produced by diet are completed in 10-14 days and, within the limits of the analytical techniques, generally parallel the decrease in plasma phospholipid and cholesterol concentrations.…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary fats on plasma lipids and lipoproteins: an hypothesis for the lipid-lowering effect of unsaturated fatty acids

Spritz¹,

Mishkel²

1969

J. Clin. Invest.

270

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A B S T R A C T Several aspects of the effects of dietary fat on plasma lipids and lipoproteins were investigated in 12 subjects during the long-term feeding of formulas containing 40% of their calories as either saturated or unsaturated fats. The changes in fatty acid composition of plasma lipids, shown previously to occur after prolonged feedings of a dietary fat, required 10-14 days to be complete and were synchronous with the effect of the fat on plasma lipid concentrations. The change in lipid concentration occurred in low but not in high density lipoproteins. The effects on lipid levels of the low density lipoproteins were found to occur with little or no effect on the concentration of the protein moiety of these lipoproteins; as a result, cholesterol-and phospholipid to protein ratios in low density lipoproteins fell during unsaturated fat feeding. The effects of dietary fat on plasma phospholipids were studied in detail: the relative amounts of phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, and lysophosphatidylcholine were unaffected by the type of dietary fat. However, the molecular species of phosphatidylcholine were markedly affected. More than 90% of the fatty acids at the a-position were saturated during both saturated and unsaturated feedings. In contrast, during unsaturated feedings, linoleate at the ,8-position outnumbered oleate by approximately 4: 1, whereas during saturated feedings these two types of fatty acids were present in nearly equal amounts.This work was presented in part at the Council on Atherosclerosis of the American Heart Association, San Francisco, Calif., 1967, and has been published in abstract form (1).

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“…After removing the contaminating esters from this alkaline ethanol-water phase by petroleum ether extraction, the ethanol-water phase was acidified and the FFA extracted with light petroleum. The solvent was then evaporated and the FFA converted into methyl esters according to the method used by Hallgren, Stenhagen, Svanborg and Svennerholm (6). Analytical separation was carried out on a Barber Colman gas chromatograph, model 10, provided with an argon ionization detector (7).…”

Section: Methodsmentioning

confidence: 99%

Extraction and Release of Individual Free Fatty Acids by the Heart and Fat Depots*

Rothlin¹,

Bing²

1961

J. Clin. Invest.

162

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It has previously been demonstrated (1) that in the postabsorptive state energy production of the heart depends largely on fatty acid metabolism; both esterified and nonesterified or free fatty acids (FFA) are taken up by the heart muscle. Although the general turnover rate of individual FFA has been studied, no investigations have been undertaken to discover their fate in the heart muscle.After administration of C14-labeled palmitic, oleic or linoleic acid which had been bound to albumin in vitro, Fredrickson and Gordon (2) found no difference in fractional turnover rate of these individual FFA in the plasma of the intact subject. General conclusions from these observations are difficult, since Bates and Olson (3) have pointed out that in the animal organism the fate of fatty acids bound to albumin in vitro may be different from that of endogenous FFA.The present study deals with the myocardial extraction of individual FFA. Since, in the course of this investigation, it was found that the rate of myocardial uptake of individual FFA differed, the work was extended to include the release of individual FFA by adipose tissue. METHODSThe patients studied in this report were free of metabolic disorders, and none of them was in congestive heart failure. Food was withheld for 12 hours prior to the test. For the animal studies, mongrel dogs were used from which food was withheld for at least 16 hours prior to the experiment. The dogs were anesthetized with pen-

show abstract

Gas Chromatographic Analysis of the Fatty Acid Composition of the Plasma Lipids in Normal and Diabetic Subjects*

Cited by 152 publications

References 18 publications

Plasma Lipid Profiles in the Winter of Hokkaido Inhabitants

Plasma Lipid Profiles in the Winter of Hokkaido Inhabitants

Effects of dietary fats on plasma lipids and lipoproteins: an hypothesis for the lipid-lowering effect of unsaturated fatty acids

Extraction and Release of Individual Free Fatty Acids by the Heart and Fat Depots*

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