Gas chromatographic and mass spectrometric analysis of molecular species of corynomycolic acids from Corynebacterium ulcerans

Possible Existence of a Novel Amphipathic Immunostimulator in the Phenol‐Water Extracts of Mycobacteriaceae

“…The cells were suspended in 1/20 M phosphate buffer (pH 7.0) and the suspension was pumped into the rotating cylinder of the mill, containing glass beads (0.25-0.50 mm diameter), and rotated at 4,500 rpm, at flow rate of 80 ml per minute. The above disruption procedure was repeated three times, and the resulting suspension of disrupted, non-acid-fast cells was centrifuged submitted to determination with the system of gas-liquid chromatograph and mass spectrometry (model M80B, Hitachi), as previously described (39).…”

Fatty and Mycolic Acid Composition of Bacterionema matruchotii and Related Organisms

“…The mycolic acids which occur in true corynebacteria (Jones, 1975;Goodfellow et al, 1976) are of relatively low molecular weight (20 , 1970;Yano & Saito, 1972). At the other extreme, mycobacteria usually contain complex mixtures of mycolic acids (Minnikin, Alshamaony & Goodfellow, 1975) of high molecular weight (60 to go carbon atoms) (Etemadi, 1967).…”

Gas Chromatography-Mass Spectrometry of Mycolic Acids as a Tool in the Identification of Medically Important Coryneform Bacteria

“…, 1972, 1978). Complete mycolic acid patterns of named corynebacteria, nocardiae and rhodococci have now been obtained by the analysis of methyl mycolates by mass spectrometry (Collins et al, 1982;Minnikin et al, 1978) and of trimethylsilyl derivatives of methyl mycolates by both GLC and GLC-MS (Corina & Sesardic, 1980;Gailley et al, 1982;Tomiyasu, 1982;Yano et al, 1972Yano et al, , 1978. The use of t-BDMS ethers of methyl mycolates is also an attractive technique since such derivatives are stable to moisture and give relatively simple mass spectra with intense ions suitable for rapid and sensitive SIR.…”

“…Mycolic acids, which are 2-alkyl,3-hydroxy long, branched fatty acids, have been found to be of particular value in the classification of actinomycetes with a wall-chemotype IV (Becker et al, 1965), that is with meso-diaminopimelic acid (meso-DAP), arabinose and galactose (Collins et a [., 1982;Corina & Sesardic, 1980;Goodfellow & Minnikin, 1977;Goodfellow et al, 1982;Minnikin et al, 1978. The mycolic acids of mycobacteria are complex in composition and may contain components with oxygen functions in addition to the 3-hydroxy system while the mycolic acids from other sources such as corynebacteria, nocardiae and rhodococci are simpler by comparison, comprising homologous mixtures of saturated and unsaturated components containing between 20 and 60 carbon atoms (Goodfellow & Minnikin, 1977;Lechevalier, 1977;Minnikin & Goodfellow, 1976Minnikin et al, 1978Yano et al, 1972Yano et al, , 1978.…”