Gas chromatographic characterization of fatty acids.Identification constants for mono- and dicarboxylic methyl esters

Miwa, Thomas K.; Mikolajczak, K. L.; Earle, F. R.; Wolff, I. A.

doi:10.1021/ac50153a010

Cited by 461 publications

(84 citation statements)

References 9 publications

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“…The FA were further identified from the equivalent chain-length (ECL) [29] determined by interpolation between two consecutive even straight-chain saturated FA and compared by reference to standards (Sigma, St. Louis, MO; Interchim, Montluçon, France) analysed under similar conditions and from mass spectra obtained by electron-impact. According to the structure, the IsoFA, anteisoFA and oBCFA represented, all together, the total branched FA (tBCFA).…”

Section: Chemical Analysesmentioning

confidence: 99%

Relationship between fatty acid composition and softness score of adipose tissue in intensively reared lambs fed with concentrate based on cereals or on beet pulp

et al. 2005

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-The aim of the study was to investigate the effect of substituting cereals by sugar beet pulp in the feed on the fatty acid (FA) composition of subcutaneous adipose tissue in indoor lambs. Forty-nine male lambs of the Lacaune dairy breed were fed from 23.6 (± 4.1) to 41.0 (± 1.9) kg live weight with hay and a pelleted concentrate containing either 80% of barley and wheat (group C) or 70% sugar beet pulp (group P). The FA composition of caudal adipose tissue sampled at slaughter was determined. The subcutaneous adipose tissue softness was not different between the two groups. The water content and the proportion of odd-numbered (oddFA) and methyl branched-chain FA (BCFA) were high but not significantly different between the two groups (respectively, 22.6% of wet weight, 7.7 and 9.7% of total FA). On the contrary, the proportion of even-numbered saturated FA (ESFA) and especially C 18:0 was low but slightly higher in the P group. The proportions of C 18:2 and C 18:3 were higher in group C than in group P (3.2 vs. 2.2%, P < 0.001 and 0.65 vs. 0.48%, P < 0.001, respectively). The softness score of adipose tissue was positively related to the water content and proportion of oddFA and BCFA. It was negatively related to the proportion of ESFA, age and live weight at the beginning of the trial. The proportion of unsaturated FA with 18 atoms of carbon did not show any relationship with the adipose tissue softness but it was influenced to some extent by the composition of the diet. lamb / sugar beet pulp / adipose tissue / fatty acid / softness index Résumé -Effet de la nature de l'énergie ingérée, céréales vs. pulpes de betteraves, sur la composition en acides gras du tissu adipeux caudal d'agneaux de bergerie. L'objectif de ce travail était de mesurer l'incidence de la distribution de pulpes de betteraves en remplacement des graines de céréales dans le régime alimentaire d'agneaux de bergerie sur la composition en acides gras (AG) du tissu adipeux de couverture. Au total, 49 agneaux mâles, de race Lacaune « lait » ont été engraissés de 23,6 (± 4,1) à 41,0 (± 1,9) kg. Ils disposaient en libre accès de foin et d'un aliment concentré aggloméré composé de 80 % d'orge et de blé (lot C) ou de 70 % de pulpe de betterave (lot P). La composition en AG d'un échantillon de tissu adipeux caudal prélevé sur chaque animal lors de l'abattage a été déterminée. L'index de fermeté ne diffère pas significativement entre les 2 groupes. La teneur en eau et la proportion des AG à chaîne carbonée impaire ou ramifiée ont été élevées et non significativement différentes (respectivement, 22,6 % du poids frais, 7,7 et 9,7 % des AG totaux). Inversement, les teneurs en AG saturés pairs et notamment en C 18:0 ont été faibles. Les teneurs en C 18:2 et C 18:3 ont été plus élevées dans le lot C que dans le lot P (3,2 vs. 2,2 %, P < 0,001 et 0,65 vs. 0,48 %, P < 0,001, respectivement). La note de fermeté des gras est liée positivement aux teneurs en eau et en AG à chaîne carbonée impaire ou ramifiée et négativement aux teneurs en AG gras satur...

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Section: Chemical Analysesmentioning

confidence: 99%

Relationship between fatty acid composition and softness score of adipose tissue in intensively reared lambs fed with concentrate based on cereals or on beet pulp

et al. 2005

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“…The identification of the components (identification system) is based on the combination of their elution characteristics on a polar and non polar column (15,16).…”

Section: Discussionmentioning

confidence: 99%

A Method for the Quantitative and Qualitative Determination of the Total Long Chain Fatty Acids in Urine

Christophe¹,

Geers²,

Verdank³

1974

Clinical Chemistry and Laboratory Medicine

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A method is described for the identification and quantitative determination of the urinary long chain fatty acids. The urinary lipids are saponified, the unsaponifiable fraction is removed, the fatty acids are extracted after acidification and converted into methyl esters. These are purified by thin layer chromatography and analysed by gas liquid chromatography. The eluted components are identified on basis of their Chromatographie behavior in three different Chromatographie systems.Eine Methode zur Identifikation, Quantitation, Bestimmung und Feststellung des Fettsäuremusters der langkettigen Fettsäuren im Urin wird beschrieben. Die Urin-Lipide werden verseift, der nicht verseifbare Anteil abgetrennt, die Fettsäuren nach Ansäuern extrahiert und in ihre Methylester umgesetzt. Diese werden durch Dünnschichtchromatographie gereinigt und gaschromatographisch bestimmt. Die Identifizierung der eluierten Komponenten erfolgt auf Grund ihres chromatographischen Verhaltens in drei verschiedenen Systemen.

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“…Several series have already been reported in the literature, such as the linear fatty acid methyl esters (FAMEs), which provide an equivalent chain length of unsaturated and branched fatty acid esters in lipid analysis, 34,35 or polychlorobiphenyl (PCB) congeners possessing distinct numbers of chlorine atoms to identify the highly toxic PCB isomers. 14 A further example is the polynuclear aromatic hydrocarbons (PAH) retention index system, which uses the numbers of rings in unsubstituted PAHs to characterize the retention behaviour of their homologues and related substituted compounds.…”

Section: Alternatives To N-alkanes As Reference Seriesmentioning

confidence: 99%

Linear retention indices in gas chromatographic analysis: a review

Zellner

Bicchi

Dugo

et al. 2008

Flavour & Fragrance J

210

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ABSTRACT:The main purpose of any chromatographic analysis is to resolve mixtures of compounds into less complex mixtures or ultimately into pure components. In addition to this function, the chromatographic system can provide retention data which serve as complementary information for the positive identification of resolved components. The need to express gas chromatographic retention data in a standardized system has long been recognized and retention index values presented to be a valuable parameter. Those values are mainly calculated by applying the equations proposed by Kováts, for isothermal analysis, and van den Dool and Kratz, for programmed gas chromatographic runs. In general, these indices denote the retention behaviour of the compounds of interest according to a uniform scale determined by a series of closely related standard substances. The use of retention indices in the flavour and fragrance field is well-documented, and they are widely applied for the comparison of results between laboratories, as well as to characterize stationary phases.

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Gas chromatographic characterization of fatty acids.Identification constants for mono- and dicarboxylic methyl esters

Cited by 461 publications

References 9 publications

Relationship between fatty acid composition and softness score of adipose tissue in intensively reared lambs fed with concentrate based on cereals or on beet pulp

Relationship between fatty acid composition and softness score of adipose tissue in intensively reared lambs fed with concentrate based on cereals or on beet pulp

A Method for the Quantitative and Qualitative Determination of the Total Long Chain Fatty Acids in Urine

Linear retention indices in gas chromatographic analysis: a review

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