Gas chromatographic determination of free amino acids and amino acids attached to transfer RNA in biological samples

Lindqvist, Leena; Mäenpää, Pekka H.

doi:10.1016/s0378-4347(00)84163-8

Cited by 18 publications

(1 citation statement)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All tissues excised in Exp. 1 were analyzed for concentrations of ornithine, putrescine, spermidine, spermine, NIacetylspermidine, and NI-acetylspermine as N-heptafluorobutyrylisobutyl derivatives (Lindqvist and Maenpaa, 1982) using a modification (Smith, 1990) of the electron capture detection capillary gas-liquid chromatographic technique of Bedford et al ( 1987). In Exp.…”

Section: Tissue Analysesmentioning

confidence: 99%

Toxicity and growth-promoting potential of spermine when fed to chicks

Sousadias

Smith

1995

Journal of Animal Science

View full text Add to dashboard Cite

Previous studies have shown that the feeding of putrescine, a biogenic amine and the precursor of the mammalian polyamines, can promote whole-body growth of chicks. The current study was undertaken to determine the effect of spermine, also a biogenic amine and the most cationic of the polyamines, under similar conditions. In Exp. 1, 120 week-old chicks were fed purified crystalline amino acid-based diets containing 0, .2, .4, .6, .8, or 1.0% spermine for 14 d. Spermine proved highly toxic and growth rates were reduced compared with controls when even .2% was fed. In Exp. 2, chicks were fed 0, .0375, .0750, or .1000% spermine. These concentrations proved less toxic than those used in Exp. 1. Supplemental dietary cysteine was then provided at 0, .3, .6, and .9% together with 0, .025, .050, or .400% spermine (Exp. 3) because depletion of cellular glutathione has been suggested as contributing to spermine's toxicity. Even high levels of cysteine supplementation did not overcome spermine's toxicity. Subsequent dietary provision of L-2-oxothiazolidine-4-carboxylic acid (OTC, Exp. 4), a cysteine prodrug, showed that depletion of cellular glutathione was not likely a cause of spermine toxicosis. A trend toward increased weight gain and feed efficiency was observed when low concentrations of spermine were fed. It was concluded, however, that dietary spermine was more toxic to chicks than was previously seen for putrescine, that any growth-promoting effects of dietary spermine are small, and that supplements of dietary cysteine or OTC are unlikely to increase these effects by overcoming spermine toxicosis.

show abstract

Section: Tissue Analysesmentioning

confidence: 99%

Toxicity and growth-promoting potential of spermine when fed to chicks

Sousadias

Smith

1995

Journal of Animal Science

View full text Add to dashboard Cite

show abstract

A review of quantitative ion exchange, high performance liquid and gas chromatographic analysis of amino acids in physiological fluids

Moodie

Shephard

Labadarios

1989

J. High Resol. Chromatogr.

View full text Add to dashboard Cite

Methodologies for quantitative analysis of amino acids in physiological fluids based on classical ion exchange, high performance liquid chromatography, and gas chromatography are analyzed. As judged by the relative number of reports on these techniques, it appears that classical ion exchange continues to be the main method of choice for amino acid determinations and only limited advantage has been taken of the benefits (e.g. lower capital outlay and running costs and shorter analysis times) offered by other techniques. More importantly, however, there appears to be insufficient quantitative evaluation of the methodologies used. As a result, the validity of many reports based on data from amino acid analysis of physiological samples may be questioned.

show abstract

Coulometric High-Performance Liquid Chromatographic Analysis of Morphine in Biological Fluids

Derendorf

Kaltenbach

1986

Journal of Pharmaceutical Sciences

View full text Add to dashboard Cite

Gas chromatographic determination of free amino acids and amino acids attached to transfer RNA in biological samples

Cited by 18 publications

References 10 publications

Toxicity and growth-promoting potential of spermine when fed to chicks

Toxicity and growth-promoting potential of spermine when fed to chicks

A review of quantitative ion exchange, high performance liquid and gas chromatographic analysis of amino acids in physiological fluids

Coulometric High-Performance Liquid Chromatographic Analysis of Morphine in Biological Fluids

Contact Info

Product

Resources

About