Gas chromatographic/mass spectrometric analysis of human serum for non-ortho (coplanar) and ortho substituted polychlorinated biphenyls using isotope-dilution mass spectrometry

Patterson, Donald G.; Lapeza, Chester R.; Barnhart, Elizabeth R.; Groce, Donald F.; Burse, Virlyn W.

doi:10.1016/0045-6535(89)90300-7

Cited by 44 publications

(10 citation statements)

References 6 publications

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“…These data should be compared with caution, however, because the U.S. (29), Japanese (10,(25)(26)(27), and Swedish (28,41) samples represent people with different background exposures and were not collected to represent a certain population group. In addition, individual PCB congeners (42,43) as well as total PCBs (44)(45)(46) have been shown to be positively associatedwithage; differencesbetweenthe sexeshave also been noted (43,46).…”

Section: Resultsmentioning

confidence: 99%

“…The laboratory procedures we used to analyze polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and PCBs in adipose tissue and serum have been previously described in detail (29,(32)(33)(34)(35)(36)(37) and only a briefdescription will be given here.…”

Section: Methodsmentioning

confidence: 99%

“…The separate analysis eliminated a previous problem with high recoveries of carbon-13-labeled PCB-77 (29). The samples were then extracted with organic solvents, and the solvent extracts were processed through a five-column cleanup procedure developed by Smith et al (38) and modified and semiautomated (33,39) by us for PCDD, PCDF, and PCB analyses.…”

Section: Sample Cleanup and Quantificationmentioning

confidence: 99%

“…We spiked the adipose tissue (32,33,36) or serum (29,34,35,37) samples with carbon-13-labeled PCDDs, PCDFs, and coplanar PCBs and in a separate analysis with carbon-13-labeled nonplanar PCBs. The separate analysis eliminated a previous problem with high recoveries of carbon-13-labeled PCB-77 (29).…”

Section: Sample Cleanup and Quantificationmentioning

confidence: 99%

“…Recent studies by Tanabe et al (10,11,14,,23,24), Kannan et al (12,25), Miyata et al (26), and Kashimoto et al (27) have found levels of PCB-77, PCB-126, and PCB-169 up to several orders (29) and adipose tissue (30) from the United States, and Beck et al (31) reported finding PCB-77 in adipose tissue and mothers' milk from Germany. We have examined additional human adipose tissues and serum from Atlanta, Georgia, to determine the distribution ofthe coplanar PCBs, PCDDs, and PCDFs in these two lipid stores ofthe human body.…”

Section: Introductionmentioning

confidence: 99%

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Levels of non-ortho-substituted (coplanar), mono- and di-ortho-substituted polychlorinated biphenyls, dibenzo-p-dioxins, and dibenzofurans in human serum and adipose tissue.

Patterson

Todd

Turner

et al. 1994

Environ Health Perspect

110

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We have measured non-ortho-substituted (coplanar) polychlorinated biphenyl (PCB) levels as well as polychlorinated dibenzo-p-dioxin (PCDD) and polychlorinated dibenzofuran (PCDF) levels in human adipose tissue and serum collected in Atlanta, Georgia. The results show that the concentrations ofthe coplanar PCBs can be more than an order of magnitude higher than the concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin. Our measurements in pooled serum collected in 1962, 1988, and 1969 show a decrease in coplanar PCB levelsfrom 1982 to 1989. Wefound that the pattern ofrelative amounts ofcoplanar PCBs in adipose tissue varied greatly from person to person unlike the PCDD and PCDF patterns, which were more nearly the same. Age was significantly correlated with the concentrations of 2,3,7,3,3'4,3,3',4,4A,5-PCB, and 3,3'4A,45,5'-PCB in adipose tissue. We also measured levels of the mono-and di-ortho chlorinesubstituted PCBs in human serum. The levels for some of these PCB congeners were three orders of magnitude higher than the coplanar PCBs, PCDDs, and PCDFs. We used the international toxicity equivalency factors (TEFs) for PCDDs and PCDFs and the TEFs proposed by Safe for PCBs to calculate the 2,3,7,-TCDD equivalents. Four PCBs (3,3',4,4,5-;

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Sample Cleanup and Quantificationmentioning

confidence: 99%

Section: Sample Cleanup and Quantificationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Levels of non-ortho-substituted (coplanar), mono- and di-ortho-substituted polychlorinated biphenyls, dibenzo-p-dioxins, and dibenzofurans in human serum and adipose tissue.

Patterson

Todd

Turner

et al. 1994

Environ Health Perspect

110

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Quality assurance of analysis of organic compounds in marine matrices: Application to analysis of chlorobiphenyls and polycyclic aromatic hydrocarbons

Boer¹,

Law²

1995

Qualilty Assurance in Environmental Monitoring

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Determination of polychlorinated biphenyls in human blood plasma by on‐line and off‐line liquid chromatography–gas chromatography

Grimvall¹,

Östman²,

Nilsson³

1995

J. High Resol. Chromatogr.

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SummaryA method for the determination of non-, mono-and di-ortho substituted polychlorinated biphenyls in human blood plasma is presented. The non-ortho CBs are isolated from the bulk of PCBs by HPLC on a dinitroanilino-propyl silica column and collected as a heart-cut fraction that is transferred on-line to GC-MS utilizing concurrent solvent evaporation technique. The on-line coupling serves to lower the limit of determination and to partly automate the sample clean-up. The di-and mono-ortho CBs are analyzed by off-line GC-ECD using two different columns. Sample pre-treatment is performed by extraction with organic solvents and fractionation on silica gel. A high reproducibility and an absolute over-all recovery of approximately 70 % is demonstrated. The method is applied to samples of blood from 5 individuals. All the samples demonstrated a similar relative concentration profile. It was also shown that three CB congeners (CB 126, CB 156 and CB 170) contributed to the major part of the toxic equivalent quantity of the PCB content in the blood.

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Gas chromatographic/mass spectrometric analysis of human serum for non-ortho (coplanar) and ortho substituted polychlorinated biphenyls using isotope-dilution mass spectrometry

Cited by 44 publications

References 6 publications

Levels of non-ortho-substituted (coplanar), mono- and di-ortho-substituted polychlorinated biphenyls, dibenzo-p-dioxins, and dibenzofurans in human serum and adipose tissue.

Levels of non-ortho-substituted (coplanar), mono- and di-ortho-substituted polychlorinated biphenyls, dibenzo-p-dioxins, and dibenzofurans in human serum and adipose tissue.

Quality assurance of analysis of organic compounds in marine matrices: Application to analysis of chlorobiphenyls and polycyclic aromatic hydrocarbons

Determination of polychlorinated biphenyls in human blood plasma by on‐line and off‐line liquid chromatography–gas chromatography

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