Determination of polychlorinated biphenyls in human blood plasma by on‐line and off‐line liquid chromatography–gas chromatography

Abstract

SummaryA method for the determination of non-, mono-and di-ortho substituted polychlorinated biphenyls in human blood plasma is presented. The non-ortho CBs are isolated from the bulk of PCBs by HPLC on a dinitroanilino-propyl silica column and collected as a heart-cut fraction that is transferred on-line to GC-MS utilizing concurrent solvent evaporation technique. The on-line coupling serves to lower the limit of determination and to partly automate the sample clean-up. The di-and mono-ortho CBs are analyzed by off-line GC-ECD using two different columns. Sample pre-treatment is performed by extraction with organic solvents and fractionation on silica gel. A high reproducibility and an absolute over-all recovery of approximately 70 % is demonstrated. The method is applied to samples of blood from 5 individuals. All the samples demonstrated a similar relative concentration profile. It was also shown that three CB congeners (CB 126, CB 156 and CB 170) contributed to the major part of the toxic equivalent quantity of the PCB content in the blood.

Solid-phase extraction or liquid chromatography coupled on-line with gas chromatography in the analysis of biological samples

“…An NPLC-GC -ECD method has been applied for the determination of levomoprol [15] and an NPLC-GC-MS method for polychlorinated biphenyls [17]. The latter method enabled a selective determination of toxic non-ortho-chlorobiphenyls in the presence of less toxic PCBs at a pg/g level.…”

“…Yet, further advantages are the shortened time of analysis and better reliability and repeatability. For biological and pharmaceutical samples, both SPE and LC have been coupled on-line with GC [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. Also other on-line techniques, such as membrane extraction and on-line LLE, have been directly coupled with GC, but these methods are not covered in this review.…”

Determination of polychlorinated biphenyls in human blood plasma by on‐line and off‐line liquid chromatography–gas chromatography

“…In this project, the non-ortho substituted PCB congeners are isolated by high performance liquid chromatography (HPLC) on a dinitroanilino-propyl silica (DNAP) column, where CB-77, CB-126 and CB-169 are eluted as one peakafterthe other CBs. WithrespecttoPCBs, thiscolumn has previously been applied only to standard solutions [9].…”

Microwave-induced Plasma Atomic Emission Spectrometer Used as the Detector for On-line Coupled High-performance Liquid Chromatography–Gas Chromatography

“…For the determination of compounds in complex matrices containing interferences, FID and ECD are not always suitable. Therefore complicated hyphenated techniques such as LC-GC-MS [8][9][10] and LC-GC-FTIR 11 have been developed.…”