Gas Chromatography/Mass Spectrometry Method to Quantify Blood Hydroxycitrate Concentration

Loe, Yu-chi Cynthia; Bergeron, Nathalie; Rodríguez, Néstor; Schwarz, Jean-Marc

doi:10.1006/abio.2001.5046

Cited by 50 publications

(24 citation statements)

References 20 publications

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“…In agreement with previous studies on soy and GCE supplements we used doses of 2 g/day, it was beyond the scope of the present study to determine whether higher doses were more effective for promoting weight-loss and improving plasma lipid profiles. Although, a previous report suggest higher doses may be futile, as oral administration of higher doses of Garcinia cambogia extract to normal subjects leads to increased urinary excretion of (-)-hydroxycitric acid, attributed to limitations in (-)-hydroxycitric acid absorption efficiency [49]. …”

Section: Discussionmentioning

confidence: 99%

Does Glycine max leaves or Garcinia Cambogiapromote weight-loss or lower plasma cholesterol in overweight individuals: a randomized control trial

Kim

Jeon

Park

et al. 2011

Nutr J

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BackgroundNatural food supplements with high flavonoid content are often claimed to promote weight-loss and lower plasma cholesterol in animal studies, but human studies have been more equivocal. The aim of this study was firstly to determine the effectiveness of natural food supplements containing Glycine max leaves extract (EGML) or Garcinia cambogia extract (GCE) to promote weight-loss and lower plasma cholesterol. Secondly to examine whether these supplements have any beneficial effect on lipid, adipocytokine or antioxidant profiles.MethodsEighty-six overweight subjects (Male:Female = 46:40, age: 20~50 yr, BMI > 23 < 29) were randomly assigned to three groups and administered tablets containing EGML (2 g/day), GCE (2 g/day) or placebo (starch, 2 g/day) for 10 weeks. At baseline and after 10 weeks, body composition, plasma cholesterol and diet were assessed. Blood analysis was also conducted to examine plasma lipoproteins, triglycerides, adipocytokines and antioxidants.ResultsEGML and GCE supplementation failed to promote weight-loss or any clinically significant change in %body fat. The EGML group had lower total cholesterol after 10 weeks compared to the placebo group (p < 0.05). EGML and GCE had no effect on triglycerides, non-HDL-C, adipocytokines or antioxidants when compared to placebo supplementation. However, HDL-C was higher in the EGML group (p < 0.001) after 10 weeks compared to the placebo group.ConclusionsTen weeks of EGML or GCE supplementation did not promote weight-loss or lower total cholesterol in overweight individuals consuming their habitual diet. Although, EGML did increase plasma HDL-C levels which is associated with a lower risk of atherosclerosis.

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Section: Discussionmentioning

confidence: 99%

Does Glycine max leaves or Garcinia Cambogiapromote weight-loss or lower plasma cholesterol in overweight individuals: a randomized control trial

Kim

Jeon

Park

et al. 2011

Nutr J

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“…(-)-Hydroxycitric acid (HCA) is widely used as an ingredient for a nutritional supplement to control body weight gain and inhibit fat accumulation in humans [23, 47-49] and animals [25, 50, 51]. (-)-HCA-containing products have been on the market for many years [18, 27].…”

Section: Discussionmentioning

confidence: 99%

(-)-Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes

Li¹,

Peng²,

Ge³

et al. 2017

Cell Physiol Biochem

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Background/Aims: (-)-Hydroxycitric acid (HCA) had been shown to suppress fat accumulation in animals and humans, while the underlying biochemical mechanism is not fully understood, especially little information is available on whether (-)-HCA regulates energy metabolism and consequently affects fat deposition. Methods: Hepatocytes were cultured for 24 h and then exposed to (-)-HCA (0, 1, 10, 50 µM), enzyme protein content was determined by ELISA; lipid metabolism gene mRNA levels were detected by RT-PCR. Results: (-)-HCA significantly decreased the number and total area of lipid droplets. ATP-citrate lyase, fatty acid synthase and sterol regulatory element binding protein-1c mRNA level were significantly decreased after (-)-HCA treatment, whereas peroxisome proliferator-activated receptor α mRNA level was significantly increased. (-)-HCA significantly decreased ATP-citrate lyase activity and acetyl-CoA content in cytosol, but significantly increased glucose consumption and mitochondrial oxygen consumption rate. (-)-HCA promoted the activity/content of glucokinase, phosphofructokinase-1, pyruvate kinase, pyruvate dehydrogenase, citrate synthase, aconitase, succinate dehydrogenase, malate dehydrogenase, NADH dehydrogenase and ATP synthase remarkably. Conclusions: (-)-HCA decreased lipid droplets accumulation by reducing acetyl-CoA supply, which mainly achieved via inhibition of ATP-citrate lyase, and accelerating energy metabolism in chicken hepatocytes. These results proposed a biochemical mechanism of fat reduction by (-)-HCA in broiler chickens in term of energy metabolism.

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“…Accordingly, the human equivalent dosage of HCA‐SX used in the present study was calculated to be 2800 mg/day, as explained in Methods and Procedures , which is significantly greater than the 1500 mg/day typically recommended in dietary supplements [31]. HCA‐SX bioavailability was found to be significantly higher in fasting individuals as compared with subjects consuming HCA‐SX in conjunction with food [36]. Accordingly, HCA‐SX was given to the study participants 30–60 min before each meal, which addresses the timing of dose.…”

Section: Introductionmentioning

confidence: 99%

Effects of a natural extract of (–)‐hydroxycitric acid (HCA‐SX) and a combination of HCA‐SX plus niacin‐bound chromium and Gymnema sylvestre extract on weight loss

Preuss

Bagchi

et al. 2004

Diabetes Obesity Metabolism

159

113

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Gas Chromatography/Mass Spectrometry Method to Quantify Blood Hydroxycitrate Concentration

Cited by 50 publications

References 20 publications

Does Glycine max leaves or Garcinia Cambogiapromote weight-loss or lower plasma cholesterol in overweight individuals: a randomized control trial

Does Glycine max leaves or Garcinia Cambogiapromote weight-loss or lower plasma cholesterol in overweight individuals: a randomized control trial

(-)-Hydroxycitric Acid Reduced Lipid Droplets Accumulation Via Decreasing Acetyl-Coa Supply and Accelerating Energy Metabolism in Cultured Primary Chicken Hepatocytes

Effects of a natural extract of (–)‐hydroxycitric acid (HCA‐SX) and a combination of HCA‐SX plus niacin‐bound chromium and Gymnema sylvestre extract on weight loss

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