Gas chromatography–mass spectrometry profile of urinary organic acids of Wistar rats orally treated with ozonized unsaturated triglycerides and ozonized sunflower oil

Jardines, Daniel; Correa, Teresa; Ledea, Oscar; Zamora, Zullyt; Rosado, Arîstides; Molerio, Jesús

doi:10.1016/s1570-0232(02)00707-9

Cited by 12 publications

(6 citation statements)

References 17 publications

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“…In the rat, 4-HNE was detected in the urine as mercapturic acid conjugates. 35,98,103,104 In normal conditions, owing to the efficiency of these processes, only submicromolar concentrations of LOP can reach organs such as bone marrow, endocrine glands, and even hypothalamic areas deprived of the blood-brain barrier where, via a variety of kinases and even a possible receptor for F 2 -isoprostanes, may act as a signaling event of an ongoing acute oxidative stress [105][106][107][108][109][110] (Fig. 5).…”

Section: A Reactions With Plasma Componentsmentioning

confidence: 99%

The ozone paradox: Ozone is a strong oxidant as well as a medical drug

Bocci

Borrelli

Travagli

et al. 2009

Medicinal Research Reviews

332

304

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After five decades characterized by empiricism and several pitfalls, some of the basic mechanisms of action of ozone in pulmonary toxicology and in medicine have been clarified. The present knowledge allows to understand the prolonged inhalation of ozone can be very deleterious first for the lungs and successively for the whole organism. On the other hand, a small ozone dose well calibrated against the potent antioxidant capacity of blood can trigger several useful biochemical mechanisms and reactivate the antioxidant system. In detail, firstly ex vivo and second during the infusion of ozonated blood into the donor, the ozone therapy approach involves blood cells and the endothelium, which by transferring the ozone messengers to billions of cells will generate a therapeutic effect. Thus, in spite of a common prejudice, single ozone doses can be therapeutically used in selected human diseases without any toxicity or side effects. Moreover, the versatility and amplitude of beneficial effect of ozone applications have become evident in orthopedics, cutaneous, and mucosal infections as well as in dentistry.

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Section: A Reactions With Plasma Componentsmentioning

confidence: 99%

The ozone paradox: Ozone is a strong oxidant as well as a medical drug

Bocci

Borrelli

Travagli

et al. 2009

Medicinal Research Reviews

332

304

View full text Add to dashboard Cite

show abstract

“…Aerobic organisms, in order to tolerate the continuous generation of aldehydic compounds have developed detoxifying systems as follows: the first is the dilution of these products in both the plasma and the extracellular fluid involving a volume of about 11 L in humans. The second is the detoxification operated by aldehyde dehydrogenase, aldose reductase and GSH-Tr [55,56] and the third is the excretion via bile and urine excretion [57-59]. The relevance of these catabolic pathways was appreciated when the half-life of infused alkenals present in ozonated blood in a patient was less than 5 min [60].…”

Section: The Pleiotropic Lop Activitiesmentioning

confidence: 99%

Ozone acting on human blood yields a hormetic dose-response relationship

2011

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The aim of this paper is to analyze why ozone can be medically useful when it dissolves in blood or in other biological fluids. In reviewing a number of clinical studies performed in Peripheral Arterial Diseases (PAD) during the last decades, it has been possible to confirm the long-held view that the inverted U-shaped curve, typical of the hormesis concept, is suitable to represent the therapeutic activity exerted by the so-called ozonated autohemotherapy. The quantitative and qualitative aspects of human blood ozonation have been also critically reviewed in regard to the biological, therapeutic and safety of ozone. It is hoped that this gas, although toxic for the pulmonary system during prolonged inhalation, will be soon recognized as a useful agent in oxidative-stress related diseases, joining other medical gases recently thought to be of therapeutic importance. Finally, the elucidation of the mechanisms of action of ozone as well as the obtained results in PAD may encourage clinical scientists to evaluate ozone therapy in vascular diseases in comparison to the current therapies.

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“…By‐product identification has already been reported ; however, the formation/decomposition dynamics of these by‐products during ozonation has not received much attention. The differences in by‐product distributions among ozonated oils from different sources could explain why the ozonation degree need not be the same for different oils.…”

Section: Introductionmentioning

confidence: 99%

Correlation of structural characterization and viscosity measurements with total unsaturation: An effective method for controlling ozonation in the preparation of ozonated grape seed and sunflower oils

Guerra-Blanco¹,

Poznyak²,

Chairez³

et al. 2015

Euro J Lipid Sci & Tech

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Ozonated oils have demonstrated promising results for clinical applications. The reaction of ozone with the unsaturated compounds of oils produces by‐products such as ozonides and poly peroxides. A deeper knowledge of the dynamics of by‐product formation is helpful in determining the required ozonation degree to obtain therapeutic effects. The aim of this paper is to show the relationship between ozonation degree and structural and viscosity changes during the ozonation of grape seed (GS) and sunflower (SF) oils. Structural characterization was done by Fourier transform infrared (FT‐IR) and hydrogen‐1 nuclear magnetic resonance (1H NMR) spectroscopy, with iso‐ozonides being identified. Viscosity showed a significant increase during ozonation, a fact associated with poly peroxide formation. We have made use of the total unsaturation (TU) method to measure the ozonation degree. The TU of non‐ozonated GS oil was found to be higher than for SF oil (5.94 and 4.49 mmol per g of oil, respectively), and their by‐product distributions were also found to differ. In GS oil, three reaction steps were observed for double‐bond conversion into iso‐ozonides and poly peroxides, while the ozonides and poly peroxides were formed in parallel in SF oil. The studies we implemented characterized the differences in the reactivities of these oils with ozone. Practical applications: In this work, we propose using the TU method to measure the ozonation degree of ozonated oils. TU experimental determination is based on the ozonation of the sample, and the ozone‐oxidizable substrate is quantified. Despite GS and SF oils having similar fatty acid compositions, they contain other unsaturated compounds specific to their vegetal sources. These compounds are also reactive with ozone, and are also quantified by the TU method. The differences in distributions of by‐products among ozonated oils from different sources could explain why the ozonation degree need not be the same for different oils. Studies like this represent a feasibility foundation for controlling the therapeutic application of ozonated oils and correctly interpreting their well‐known clinical effects. Ozonated vegetable oils have many interesting applications in the food, cosmetic, and pharmaceutical industries, as well as in medicine. A deeper knowledge of the dynamics of by‐product formation is helpful in determining the required ozonation degree to obtain therapeutic effects. In this work, we propose using the total unsaturation method to measure the ozonation degree of ozonated oils. We showed the relationship between ozonation degree and structural and viscosity changes during the ozonation of grape seed and sunflower oils. The differences in distributions of by‐products among ozonated oils from different sources could explain why the ozonation degree need not be the same for different oils.

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Gas chromatography–mass spectrometry profile of urinary organic acids of Wistar rats orally treated with ozonized unsaturated triglycerides and ozonized sunflower oil

Cited by 12 publications

References 17 publications

The ozone paradox: Ozone is a strong oxidant as well as a medical drug

The ozone paradox: Ozone is a strong oxidant as well as a medical drug

Ozone acting on human blood yields a hormetic dose-response relationship

Correlation of structural characterization and viscosity measurements with total unsaturation: An effective method for controlling ozonation in the preparation of ozonated grape seed and sunflower oils

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