Gas chromatography of some polymethoxylated flavones and their determination in orange peel oils

Gaydou, Émile M.; Berahia, Tahar; Wallet, Jean‐Claude; Bianchini, Jean‐Pierre

doi:10.1016/s0021-9673(00)91457-2

Cited by 22 publications

(11 citation statements)

References 16 publications

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“…Flavonoids can be analyzed by gas chromatography (GC) (Gaydou et al, 1991;Greenaway and Whatley, 1990), high-performance liquid chromatography (HPLC) (Chang et al, 2006;Svedstrom et al, 2002Svedstrom et al, , 2006Wang et al, 2004) and capillary zone electrophoresis (CZE) (Marchart and Kopp, 2003;Urbonaviciute et al, 2006;Wang and Huang, 2004) or micellar electrokinetic capillary chromatography (MEKC) (Wang et al, 2003;Xie et al, 2002). The quality control of hawthorn fruit and leaf are also defined in China Pharmacopoeia (Vol.…”

Section: Introductionmentioning

confidence: 99%

Quantitative LC/MS/MS method and in vivo pharmacokinetic studies of vitexin rhamnoside, a bioactive constituent on cardiovascular system from hawthorn

Liang

Zhang

et al. 2007

Biomedical Chromatography

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A simple and accurate liquid chromatography coupled with tandem mass spectrometry method was developed for determination and in vivo pharmacokinetic studies of vitexin rhamnoside in rat plasma. After protein precipitation using methanol, the analytes were separated by a Luna C(18) column with an isocratic elution and analyzed by mass spectrometry in multiple reaction monitoring mode using the respective negative ion at m/z 577.2-293.0 for vitexin rhamnoside and m/z 593.2-413.0 for internal standard (IS) vitexin glucoside. The method was validated systematically within the concentration range 5-5000 microg/L (R > 0.996) and the lower limit of quantitation was 5 microg/L. Acceptable precision and accuracy were acquired for concentrations over the standard curve range. It was further applied to assess pharmacokinetics and bioavailability of vitexin rhamnoside after intravenous and oral administration to rats. The oral bioavailability of vitexin rhamnoside was only 3.57%, which indicated that vitexin rhamnoside had poor absorption or underwent extensive first-pass metabolism. Practical utility of this new LC/MS/MS method was confirmed in pilot pharmacokinetic studies in rats following both intravenous and oral administration.

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Section: Introductionmentioning

confidence: 99%

Quantitative LC/MS/MS method and in vivo pharmacokinetic studies of vitexin rhamnoside, a bioactive constituent on cardiovascular system from hawthorn

Liang

Zhang

et al. 2007

Biomedical Chromatography

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“…SFC (supercritical fluid chromatography) was used for rapid separation of polymethoxyflavones on a micro-gram scale (Morin et al, 1991). From orange peels, 27 polymethoxyflavones were separated and identified using GC (gas chromatography) with a capillary column coated with OV-1 (Gaydou, 1991). The other method employed in previous isolation of nobiletin is column chromatography on silica gel (Sasaki and Yoshizaki, 2002;Yu, 2004).…”

Section: Introductionmentioning

confidence: 99%

Nobiletin: efficient and large quantity isolation from orange peel extract

2005

Biomed. Chromatogr.

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It is known that nobiletin possesses anticancer, antiviral and anti-inflammatory activities. Recently, the demand for nobiletin in large quantities and high purity has increased. However, conventional normal-phase silica gel chromatography and C 18 -reverse-phase separation methods cannot satisfy the requirement of pure and gram scale nobiletin in a timely manner. In exploring the composition and the biological activities of polymethoxyflavones from sweet orange (Citrus sinensis) peel, we developed an efficient isolation method for nobiletin. By employing this methodology, pure nobiletin, in gram quantities, was obtained in only one purification cycle. The orange peel extract was loaded onto a silica gel flash column and eluted by a mixed solvent system of ethyl acetate and hexanes, and the fractions collected. Upon combination of the eluted fractions, mainly containing nobiletin and 5,6,7,4′-tetramethoxyflavone, and concentration under reduced pressure, the resultant residue was loaded onto a Regis chiral column. Gram amounts of nobiletin and 5,6,7,4′-tetramethoxyflavone were then eluted with ethanol and hexanes, respectively.

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“…Under isothermal conditions at 280 8C the elution of all six PMFs was complete in 55 minutes [14]. A similar separation, albeit with significant elution order changes used hydrogen carrier gas and a 30 meter, 0.25 mm i.d., DB-1 (methyl silicone) column at 310 8C; the analysis was complete in under five minutes, Figure 2.a.…”

Section: Chromatographic Separationmentioning

confidence: 94%

“…These compounds have been used in taxonomic classification studies [12], including a taxonomic differentiation between oranges and mandarins by HPLC using the six PMFs in Figure 1 [13]. One study of PMFs in orange peel oil by GC with FID detection was reported in 1991 [14]. Isothermal conditions were used to identify the six predominant PMFs in orange oils from Brazil, Israel and Morocco.…”

Section: Introductionmentioning

confidence: 99%

GC/MS Analysis of Polymethoxyflavones in Citrus Oils

Stremple¹

1998

J. High Resol. Chromatogr.

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Gas chromatography of some polymethoxylated flavones and their determination in orange peel oils

Cited by 22 publications

References 16 publications

Quantitative LC/MS/MS method and in vivo pharmacokinetic studies of vitexin rhamnoside, a bioactive constituent on cardiovascular system from hawthorn

Quantitative LC/MS/MS method and in vivo pharmacokinetic studies of vitexin rhamnoside, a bioactive constituent on cardiovascular system from hawthorn

Nobiletin: efficient and large quantity isolation from orange peel extract

GC/MS Analysis of Polymethoxyflavones in Citrus Oils

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