Gaschromatographische Bestimmung von Chloramphenicol‐Rückständen in tierischem Material

Abstract

On the basis of the relevant literature and of their own experimental work, the authors describe extraction, cleaning and derivatization procedures for the determination of chloramphenicol in milk, muscular substance, urine and bile. Chloramphenicol was determined as bis-(trimethylsilyl)-ether using a gas chromatograph fitted with an electron capture detector. To evaluate the analytical techniques, the authors estimated the sensitivity, accuracy and precision for residue amounts of 0.1 and 0.05 mg of chloramphenicol/kg of material under investigation. The limit of detection was 0.02 mg/kg for meat, and 0.01 mg/kg for milk, urine and bile.

Antibiotic residues in milk: Past, present, and future

“…Antibiotic residue in milk was first detected in 60s [20] followed by a swelling trend with a stiff increase in detection after 2000 (188) [21–208] The related published literatures from 2000 to 2009 is 81 (36.16%) in number [128–208], which is more than double in comparison with previous four decades collectively, where number was 36 (16.07%) in total [19,20,209–242] The ongoing decade merely comprises 47.77% research studies (107 in number) among the last 57 years [21–127], which clearly indicates the increasing trends with concern about antibiotic residue in milk and detection accordingly. The results are shown in Figure 2.…”

“…Original articles, published throughout the period of January 1965 to December 2017, were searched using a bibliographic database called “ISI Web of Science” [18] The published literatures were searched using the following keywords: (detection and quantification) or only “detection” or only “quantification” (antibiotic/antimicrobial) and (cow/cattle/bovine, sheep/ovine, goat/caprine, mare, and animal). To find out the maximum articles of similar concept, avoiding the risk of missing due to plural word or multiple words, a sign “*” was used in accordance with the published guidelines [19] The searched items or publications were thoroughly checked and downloaded for detail and critical reviewing later. Only the original research data containing publications, written in English language, were included for our reviewing.…”