Gaseous compounds of soybean tissue cultures: Carbon dioxide and ethylene evolution

The growth of single roots ofArtemisia annua in nutrient mist reactors

“…Although it is usually less important than O 2 , CO 2 can affect the health and growth kinetics of hairy roots (DiIorio et al, 1992b;Haigh, 1993;Weathers and Zobel, 1992;Weathers et al, 1997;Zobel, 1987). Zobel (1987) proposed that root tissues require a certain level of CO 2 in their environment in order to function normally.…”

“…Although it is usually less important than O 2 , CO 2 can affect the health and growth kinetics of hairy roots (DiIorio et al, 1992b;Haigh, 1993;Weathers and Zobel, 1992;Weathers et al, 1997;Zobel, 1987). Zobel (1987) proposed that root tissues require a certain level of CO 2 in their environment in order to function normally. DiIorio et al (1992b) showed that biomass yield increased 2.5 times for hairy root cultures of Carthamus tinctorius grown on Gelrite with 1% CO 2 in the headspace gas compared with room air and 1.4 times more for Beta vulgaris at 1.5% CO 2 .…”

“…DiIorio et al (1992b) showed that biomass yield increased 2.5 times for hairy root cultures of Carthamus tinctorius grown on Gelrite with 1% CO 2 in the headspace gas compared with room air and 1.4 times more for Beta vulgaris at 1.5% CO 2 . A low level of CO 2 (∼1%) supplementing the carrier gas has also been shown to promote healthy root tissue growth in mist reactors (DiIorio et al, 1992b;Zobel, 1987), although level of response varies with species. CO 2 addition to B. vulgaris roots during the first week of growth in mist, while not altering the growth rate, did affect the overall kinetics by eliminating the initial lag phase of mist cultures .…”

Applications of Continuous and Steady-State Methods to Root Biology

“…They reported further that gibberellic acid applications to the roots could substitute for root zone [C0 2 l enrichment (Arteca and Dong 1981). Zobel (1975Zobel ( , 1987, see also Abeles 1973, Constabel et al 1977 has found that [C0 2 ] and ethylene competitively affect tissue cultures and cytodifferentiation. Zobel (1975Zobel ( , 1987, see also Abeles 1973, Constabel et al 1977 has found that [C0 2 ] and ethylene competitively affect tissue cultures and cytodifferentiation.…”

Design, Development, And Applications Of Mist Bioreactors For Micropropagation And Hairy Root Culture

“…An important advantage of gas-phase systems such as a nutrient mist bioreactor (mist reactor) when used for micropropagation is the potential for precise control of the gas composition and relative humidity surrounding the plants because these parameters can significantly affect multiplication rates, rooting, and acclimatization [19,20]. Design and development of an effective and inexpensive mist reactor for micropropagation, however, presents engineering challenges unique to this application.…”