Gasoline engine exhaust analysis using constant energy synchronous luminescence spectrometry

Files, Leigh Ann; Jones, Bradley T.; Hanamura, S.; Winefordner, J. D.

doi:10.1021/ac00298a037

Cited by 36 publications

(14 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ability of PLS and Multiple Linear Regression (MLR) methods, to quantify fluorene in mixtures by using constant wavelength synchronous spectrofluorimetry, was described and evaluated. This is a very important subject due to other papers have published analysis of fluorene in presence of compounds capable of quenching its fluorescence signal without taking account this possibility [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Modeling of inner filter effect in synchronous spectrofluorimetry by using partial least squares

et al. 2000

View full text Add to dashboard Cite

Abstract. This paper deals with a very common problem on spectrofluorimetry: the inner-filter effect. This limitation has made spectrofluorimetry into a disadvantageous technique for the quantification of individual compounds in complex mixtures, however, a lot of papers have quantified compounds as fluorene in mixtures where other Polycyclic Aromatic Hydrocarbons (PAHs) absorb part of the energy emitted by fluorene without taking account this limitation. The inner-effect filter for fluorene is easily detectable in spectrofluorimetric measurements in mixtures where there are compounds, such as benzo(k)fluoranthene, indeno(1,2,3-cd)pyrene and pyrene. The application of methods, such as Multiple Linear Regression (MLR) to the quantification of fluorene in mixtures containing compounds capable to quench its signal, provides high errors in the analytical results. This point is carefully treated in our paper. The precise and accurate quantification of fluorene in presence of benzo(k)fluoranthene, indene(1,2,3-cd)pyrene and pyrene, was achieved by constant-wavelength synchronous fluorimetry in combination with Partial Least Squares (PLS) calibration.

show abstract

Section: Introductionmentioning

confidence: 99%

Modeling of inner filter effect in synchronous spectrofluorimetry by using partial least squares

et al. 2000

View full text Add to dashboard Cite

show abstract

“…4 Human exposure to BaP and PAHs can occur through ingestion of food, contaminated air and water. 5 Owing to their cancer-inducing activity, the need to identify and determine these compounds has led to the establishment of numerous methods for their simultaneous determination. [5][6][7][8][9][10][11][12][13][14][15] Fluorescence spectroscopy is a powerful tool in quantitative analysis owing to its great sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Resolution of benzo[a]pyrene in complex mixtures of other polycyclic aromatic hydrocarbons. Comparison of two spectrofluorimetric methods applied to water samples

Eiroa

Blanco

López-Mahı́a

et al. 2000

Analyst

View full text Add to dashboard Cite

Two spectrofluorimetric methods, second-derivative constant-energy synchronous luminescence (SDCESL) and constant-wavelength synchronous luminescence (CWSL) in combination with multiple linear regression (MLR), for the quantification of benzo[a]pyrene (BaP) at sub-ng mL-1 levels, in the presence of benzo[b]fluoranthene (BbFt), benzo[k]fluoranthene (BkFt), benzo[ghi]perylene (BghiP) and indeno[1,2,3-cd]pyrene (IP), were developed and compared in detail. SDCESL presents lower limits of detection and quantification than CWSL/MLR and also gives more exact and precise results for levels close to the quantification limit. For BaP, SDCESL achieved quantification limits of 0.019 ng mL-1 in river waters and 0.007 ng mL-1 in drinking waters. This work offers a sensitive, precise, accurate, rapid, simple and economic methodology for monitoring BaP in waters for public consumption, meeting all the requirements of the EC Directive 98/83/CE that fixes the maximum admissible limit for this polycyclic aromatic hydrocarbon in drinking waters at of 0.010 ng mL-1.

show abstract

“…This approach has been successfully applied in PAH analysis. [9][10][11][12][13][14][15][16][17][18] In CESLS, the synchronous scanning can be optimized for a specific absorption-fluorescence transition by setting a ∆ -υ value equal to the vibrational energy difference. CESLS is more effective for narrowing the spectral band and reducing Raman scattering interference than general SFS.…”

mentioning

confidence: 99%

Micelle-Sensitized Constant-Energy Synchronous Fluorescence Spectrometry for the Simultaneous Determination of Pyrene, Benzo[a]pyrene and Perylene

Lin

2005

ANAL. SCI.

View full text Add to dashboard Cite

The identification and quantification of polycyclic aromatic hydrocarbons (PAHs) in the environment are of great interest due to their carcinogenic and mutagenic characteristics. 1 Chromatographic methods, like GC-MS, HPLC with UV-visible or fluorescence detector etc., are time-consuming, expensive and require a separation procedure before analysis. Because of the strong intrinsic fluorescence emission of PAHs, fluorescence spectroscopy could provide a highly sensitive method for the analysis of PAHs.Synchronous fluorescence spectroscopy (SFS) has been known to be a simple and selective method for the simultaneous determination of multicomponent samples, 2-6 while maintainning the fluorometric sensitivity.Inman and Winefordner 7,8 described a kind of synchronous scanning mode, namely constant-energy synchronous luminescence spectrometry (CESLS), in which a constant energy difference (∆ -υ) between excitation and emission monochromators is maintained as each is scanned through the spectral region of interest. This approach has been successfully applied in PAH analysis. [9][10][11][12][13][14][15][16][17][18] In CESLS, the synchronous scanning can be optimized for a specific absorption-fluorescence transition by setting a ∆ -υ value equal to the vibrational energy difference. CESLS is more effective for narrowing the spectral band and reducing Raman scattering interference than general SFS. The fluorescence excitation and emission spectra of PAHs are generally characterized with similar spaced vibrational features. CESLS can attain the same spectral features with a single scan as conventional constant-wavelength synchronous fluorescence spectroscopy in PAH multicomponent analysis.However, resonance electronic energy transfer in mixture of PAHs has limited the application of SFS for PAH analysis. 19 For example, there is appreciable energy transfer from pyrene to perylene in a solution containing both. 20,21 The micellization of a PAHs mixture solution can help resolve this problem by isolating fluorophores in different micelles. 22,23 Micelles can also easily solubilize PAHs and increase the fluorescence intensities of fluorophores, thereby increasing the sensitivity. The analysis of certain PAHs mixtures in micellar media has been made using conventional fluorometric techniques, 22,24,25 as well as constant-wavelength and variable-angle synchronous fluorometry. 23,26,27 It can be expected that CESLS would encounter the same problem of energy transfer, and micelles would be a good solution, as in other fluorometric methods.In this paper, combined use of micellar media and constantenergy synchronous fluorescence spectroscopy for the simultaneous determination of pyrene, perylene and benzo [a]pyrene in water samples is described.To our knowledge, this is the first report on the application of micellar media in CESLS. The method exempted the resonance electronic energy transfer among the different PAHs, and thus allowed their simultaneous identification and quantitative determination via a single spectrum. Because th...

show abstract

Gasoline engine exhaust analysis using constant energy synchronous luminescence spectrometry

Cited by 36 publications

References 34 publications

Modeling of inner filter effect in synchronous spectrofluorimetry by using partial least squares

Modeling of inner filter effect in synchronous spectrofluorimetry by using partial least squares

Resolution of benzo[a]pyrene in complex mixtures of other polycyclic aromatic hydrocarbons. Comparison of two spectrofluorimetric methods applied to water samples

Micelle-Sensitized Constant-Energy Synchronous Fluorescence Spectrometry for the Simultaneous Determination of Pyrene, Benzo[a]pyrene and Perylene

Contact Info

Product

Resources

About