Gastric cardia adenocarcinoma microRNA profiling in Chinese patients

Abstract

Gastric cardia adenocarcinoma (GCA), which occurs at the gastroesophageal boundary, is one of the most malignant types of cancer. Over the past 30 years, the incidence of GCA has increased by approximately sevenfold, which has a more substantial increase than that of many other malignancies. However, as previous studies mainly focus on non-cardia gastric cancer, until now, the mechanisms behind GCA remain largely unknown. MicroRNAs (miRNAs) have been shown to play pivotal roles in carcinogenesis. To gain insight into the molecular mechanisms regulated by miRNAs in GCA development, we investigated miRNA expression profiles using 81 pairs of primary GCAs and corresponding non-tumorigenic tissues. First, 21 pairs of samples were used for microarray analysis, and then another 60 pairs of samples were used for further analysis. Our results showed that 464 miRNAs (237 upregulated, 227 downregulated, false discovery rate FDR <0.05) were differently expressed between GCA and non-tumor tissues. Pearson test and pathway analysis revealed that these dysregulated miRNA correlated coding RNAs may have effects on several cancer-related pathways. Four miRNAs (miR-1244, miR-135b-5p, miR-3196, and miR-628-3p) were found to be associated with GCA differentiation. One miRNA, miR-196a-5p, was found to be associated with age of GCA onset. Further, survival analysis showed that the expression level of miR-135b-5p was associated with GCA survival. Taken together, our study first provided the genome-wide expression profiles of miRNA in GCA and will be good help for further functional studies.

Candidate diagnostic miRNAs that can detect cancer in prostate biopsy

“…Many miRNAs identified as dysregulated in the present study have also been reported as altered in other cancer types, including breast cancer (miR‐374b‐5p, miR‐429, miR‐200b‐5p, miR‐200b‐3p, miR‐187‐3p, miR‐196a‐5p, miR‐183‐5p, miR‐21‐5p, and miR‐182‐5p); gastric cancer (miR‐374b‐5p, miR‐200c‐3p, miR‐18b‐5p, miR‐196a‐5p, miR‐21‐5p, and miR‐20a‐3p); ovarian cancer (miR‐141‐5p, miR‐182‐5p, miR‐483‐3p, and miR‐200c‐3p); hepatocellular carcinoma (miR‐135a‐5p, miR‐187‐3p, miR‐148a‐5p, miR‐200a‐3p, and miR‐9‐3p); colorectal cancer (miR‐9‐3p, miR‐135a‐5p, miR‐200c‐3p, miR‐200b‐3p, and miR‐182‐5p); thyroid cancer (miR‐7‐5p and miR‐9‐3p); lung cancer (miR‐200b‐5p, miR‐200b‐3p, miR‐9‐5p, miR‐200a‐3p, and miR‐21‐5p); pancreatic cancer (miR‐200b‐3p, miR‐483‐3p, and miR‐183‐5p); chronic lymphocytic leukemia (miR‐4521, miR‐7‐5p, and miR‐182‐5p); Hodgkin lymphoma (miR‐876‐5p); cervical cancer (miR‐429); head and neck squamous cell carcinoma (miR‐200b‐5p); and bladder cancer (miR‐148a‐3p) …”

“…-5p)[39][40][41][42][43][44][45][46] ; gastric cancer (miR-374b-5p, miR-200c-3p, miR-18b-5p, miR-196a-5p, miR-21-5p, and miR-20a-3p)[47][48][49][50][51] ; ovarian cancer (miR-141-5p, miR-182-5p, miR-483-3p, and miR-200c-3p)[52][53][54][55] ; hepatocellular carcinoma (miR-135a-5p, miR-187-3p, miR-148a-5p, miR-200a-3p, and miR-9-3p)[56][57][58][59] ; colorectal cancer…”

MicroRNA-3196 is inhibited by H2AX phosphorylation and attenuates lung cancer cell apoptosis by downregulating PUMA

“…Previous studies showed that miR-3196 is downregulated in basal cell carcinoma [15] and in breast cancer patients with lymph node metastasis [16], whereas it is upregulated in papillary thyroid carcinoma patients with lung metastases [17]. miR-3196 was reported to be also associated with gastric cardia adenocarcinoma differentiation [18]. In the present study, we showed that miR-3196 is involved in apoptosis in lung cancer cells and inhibits VP-16 induced apoptosis by downregulating PUMA.…”

Risk factors predisposing to cardia gastric adenocarcinoma: Insights and new perspectives

“…The miR‐196a‐5p was correlated with age of CGA onset. Survival analysis revealed that the miR‐135b‐5p expression level was correlated with survival of CGA . Table presents the results obtained from microarray analysis of miRNAs in CGA.…”