Gastrin Exerts Pleiotropic Effects on Human Melanoma Cell Biology

Mathieu, Véronique; Mijatovic, Tatjana; Damme, Marc Van; Kiss, Róbert

doi:10.1593/neo.05379

Cited by 34 publications

(48 citation statements)

References 49 publications

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“…Despite worldwide research and major development efforts in oncology therapeutics, the relentless prevalence and lethal nature of cancer persist for about 50% of patients, with only incremental overall improvements in treatment outcomes, for certain types of cancers including gliomas [1,2], melanomas [3,4], non-small-celllung cancers (NSCLC) [5,6], esophageal cancers [7,8], head & neck cancers [9,10] and pancreas cancers [11,12]. All of these cancer types have in common the fact that they display intrinsic resistance to pro-apoptotic stimuli [1][2][3][4][5][6][7][8][9][10][11][12], an important feature since >85% of the current chemotherapeutics used by oncologists to fight cancer are pro-apoptotic agents [13][14][15][16][17].…”

Section: Resistance Of Cancer Cells To Conventional Chemotherapeuticsmentioning

confidence: 99%

“…All of these cancer types have in common the fact that they display intrinsic resistance to pro-apoptotic stimuli [1][2][3][4][5][6][7][8][9][10][11][12], an important feature since >85% of the current chemotherapeutics used by oncologists to fight cancer are pro-apoptotic agents [13][14][15][16][17]. In addition, metastatic cancer cells are also naturally resistant to pro-apoptotic agents because they must resist anoïkis, thus pro-apoptotic agents, during their metastatic journey [13,18].…”

Section: Resistance Of Cancer Cells To Conventional Chemotherapeuticsmentioning

confidence: 99%

“…Essentially, the most important part of the binding site is represented by the first extra-cellular loop of the subunit [30][31][32][33]. To date, four (1)(2)(3)(4) and three isoforms ( 1-3) have been characterized in mammalian cells with tissue-dependent distribution [24][25][26][27][28]. All possible , combinations result in catalytically competent enzymes, indicating that multiple Na + /K + -ATPase isozymes can operate in the cell [24][25][26][27][28].…”

Section: The Na + /K + -Atpase (The Sodium Pump)mentioning

confidence: 99%

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Cardiotonic Steroids-Mediated Targeting of the Na+/K+-ATPase to Combat Chemoresistant Cancers

Mijatovic¹,

Dufrasne²,

Kiss

2012

CMC

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A large proportion of cancer patients fail to respond to conventional chemotherapy because of the intrinsic resistance of their cancer to pro-apoptotic stimuli and/or the acquisition of a multidrug resistant (MDR) phenotype during chronic chemotherapy. A new angle in chemotherapeutics against these cancer types associated with dismal prognoses would be the targeting of specific ion channels and pumps over expressed by cancer cells as compared to normal cells. Several reports suggest that the alpha subunits of the Na(+)/K(+)-ATPase (referred as sodium pump from now on) could be such targets, using cardiotonic steroids (CS) including cardenolides and bufadienolides. A significant proportion of non-small-cell-lung cancers (NSCLCs), glioblastomas (GBMs), melanomas and kidney cancers overexpresses the alpha-1 subunit of the sodium pump as compared to corresponding normal tissues, while colon cancers overexpress the alpha-3 subunit. Thus, a deeper knowledge of the structure-activity relationship (SAR), in terms of CS-mediated anticancer effects, to the sodium pump alpha subunits might enable the identification of potent anticancer agents with limited cardiotoxicity. The current review provides an in depth SAR analysis with respect to cardenolide- versus bufadienolide-mediated anticancer effects. Moreover, pharmacological data from in vitro and in vivo experiments, as well as pre-clinical and clinical trials regarding cardenolides to combat cancers associated with dismal prognoses are presented.

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Section: Resistance Of Cancer Cells To Conventional Chemotherapeuticsmentioning

confidence: 99%

Section: Resistance Of Cancer Cells To Conventional Chemotherapeuticsmentioning

confidence: 99%

Section: The Na + /K + -Atpase (The Sodium Pump)mentioning

confidence: 99%

See 1 more Smart Citation

Cardiotonic Steroids-Mediated Targeting of the Na+/K+-ATPase to Combat Chemoresistant Cancers

Mijatovic¹,

Dufrasne²,

Kiss

2012

CMC

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“…were evaluated for their in vitro growth inhibitory activities against five human tumour cell lines, including the human A549 non-small cell lung cancer, [22] the Hs683 oligodendroglioma and U373 glioblastoma, [23] the MCF-7 breast cancer, [24] and the SKMEL28 melanoma [25] cell lines, and against the B16F10 murine melanoma cell line. [26] The inhibitory growth activities of the compounds under study were determined by means of the MTT colorimetric assay.…”

Section: Spirosphaerolmentioning

confidence: 99%

Diterpenes with Unprecedented Skeletons from the Red Alga Sphaerococcus coronopifolius

et al. 2015

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Three previously unknown diterpenes -spirosphaerol (1), anthrasphaerol (2) and corfusphaeroxide (3) -have been isolated from the red alga Sphaerococcus coronopifolius. Their structures were elucidated by spectral analysis, including [a]General: Optical rotations were measured with a Perkin-Elmer model 341 polarimeter and a 10 cm cell. UV spectra were recorded with a Shimadzu UV-160A spectrophotometer. IR spectra were obtained with a Bruker Tensor 27 spectrometer. NMR spectra were recorded with Bruker AC 200 and Bruker DRX 400 spectrometers. Chemical shifts are given on a δ scale (ppm) with reference to the solvent signals. The 2D experiments (HSQC, HMBC, COSY, NOESY) were performed with use of standard Bruker pulse sequences. High-resolution ESI mass spectra were measured in positive mode with a Thermo Scientific LTQ Orbitrap Velos mass spectrometer (Institute of Biology, Medicinal Chemistry and Biotechnology, National Hellenic Research Foundation). Low-resolution EI and CI mass spectra were measured with a Thermo Electron Corporation DSQ mass spectrometer and a Direct-Exposure Probe, with CH 4 as reagent gas. Vacuum liquid chromatography was performed with Kieselgel 60 (Merck), and gravity column chromatography was performed with Kieselgel 60H (Merck). Thinlayer chromatography (TLC) was performed with Kieselgel 60 F 254 aluminium support plates (Merck), and spots were visualized after spraying with H 2 SO 4 in MeOH (15 %, v/v) reagent followed by heating. HPLC separations were conducted with an Agilent 1100 model equipped with refractive index detector and a Kromasil 100 C18 5µ (250 ϫ 8 mm) HPLC reversed-phase column or a Supelco-Sil 5µ (250 ϫ 10 mm) HPLC normal-phase column. The structures in Figures 1, 2, and 3 were generated and optimised (energy: 37.26, 33.73 and 43.30 kcal mol -1 , respectively) by use of "HyperChem™ Professional 8.0.8" molecular modelling and simulation software (force field: MM+; optimisation algorithm: Polak-Ribiere). Plant Material: S. coronopifolius was collected by SCUBA diving in Liapades Bay, Corfu, Greece, at a depth of 8-15 m in June 2009. A voucher specimen is kept at the Herbarium of the Laboratory of Pharmacognosy and Chemistry of Natural Products, University of Athens (ATPH/MP0140).Extraction and Isolation: S. coronopifolius was initially freeze-dried (149.2 g dry weight) and then exhaustively extracted with CH 2 Cl 2 / MeOH mixtures (3:1) at room temperature. The combined extracts were concentrated to give a dark green residue (4.2 g), which was later subjected to vacuum liquid chromatography on silica gel, with use of a 2 % step gradient of cyclohexane/EtOAc elution sequence. Fraction F4 (4 % EtOAc in cyclohexane, 2.35 g) was fractionated by gravity column chromatography, with use of a 1 % step gradient of cyclohexane/EtOAc. Fraction F4.3 (8 % EtOAc in cyclohexane, 1.02 g) was further separated by reversed-phase HPLC, with use of www.eurjoc.org 2852 CH 3 CN as the mobile phase. Peak F4.3.9 (t R 11.4 min, 11.7 mg) was further purified by normal-phase HPLC, with use of 15 % Et...

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“…Histologic slides were stained with hematoxylin and eosin for morphologic analyses and vessel counts, as described previously. 30 Immunohistochemical analyses also were carried out as detailed above. All in vivo experiments described in this study were performed in accordance with Authorization LA1230509 from the Animal Ethics Committee of the Federal Department of Health, Nutritional Safety, and the Environment (Brussels, Belgium).…”

Section: Animal Modelsmentioning

confidence: 99%

Considering temozolomide as a novel potential treatment for esophageal cancer

Bruyère¹,

Lonez

Duray

et al. 2010

Cancer

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BACKGROUND: C-X-C ligand (CXCL) chemokines exert major roles in the biologic aggressiveness of esophageal cancer. In the current study, the authors investigated temozolomide (TMZ)-induced effects on activity of the CXCL chemokine network in human esophageal cancer cells. To the authors' knowledge, TMZ has not been investigated previously in experimental or clinical esophageal cancers. METHODS: A complete mapping of CXCL chemokines and their receptor messenger RNA was performed in 2 established human esophageal cancer cell lines (OE21 and OE33) and in 4 surgical samples from patients with esophageal carcinoma. The analyses pointed out the potential importance of CXCL2, and monitoring CXCL2 with quantitative videomicroscopy indicated that its biologic activity was silenced in OE21 esophageal cancer cells. TMZ-mediated antitumor activity was determined in vivo in an OE21 metastatic nude mice xenograft model. RESULTS: The messenger RNA levels of CXC chemokines and their receptors were similar in both cell lines and in the 4 surgical specimens. CXCL2 depletion by small interfering RNA (siRNA) displayed marked effects on the proliferation of transfected OE21 cells. Chronic in vitro TMZ treatment of OE21 and OE33 cells markedly decreased CXCL2 and CXCL3 secretion. In vivo, TMZ induced significant delays in OE21 xenograft tumor development and improved the survival of OE21 xenograft-bearing mice, whereas cisplatin did not. Analyses performed on tissue samples from in vivo experiments revealed that TMZ also impaired tumor angiogenesis. CONCLUSIONS: The current study emphasized the role of proangiogenic chemokines in esophageal cancer biology and indicated the possibility of using TMZ as a clinically compatible drug to impair the actions of the CXCL chemokine network in esophageal cancers.

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Gastrin Exerts Pleiotropic Effects on Human Melanoma Cell Biology

Cited by 34 publications

References 49 publications

Cardiotonic Steroids-Mediated Targeting of the Na+/K+-ATPase to Combat Chemoresistant Cancers

Cardiotonic Steroids-Mediated Targeting of the Na+/K+-ATPase to Combat Chemoresistant Cancers

Diterpenes with Unprecedented Skeletons from the Red Alga Sphaerococcus coronopifolius

Considering temozolomide as a novel potential treatment for esophageal cancer

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