Gastrodin protects against MPP+-induced oxidative stress by up regulates heme oxygenase-1 expression through p38 MAPK/Nrf2 pathway in human dopaminergic cells

Jiang, Genling; Hu, Yuqin; Liu, Lanlan; Cai, Jiali; Peng, Cheng; Li, Qinglin

doi:10.1016/j.neuint.2014.06.003

Cited by 103 publications

(65 citation statements)

References 36 publications

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“…RNAi-mediated Nrf2 knockdown diminished the cytoprotective effects of 20C, which strongly suggests that the Nrf2/ HO-1/NQO1 pathway was activated. These results are similar to previous studies demonstrating that Nrf2 plays a key role in protecting cells against oxida tive stress [34][35][36] . However, in the MTT assay, 1 μmol/L 20C had a beneficial effect against rotenone-induced apoptosis in Nrf2-knockdown PC12 cells, which indicated that, 20C might activate other targets, in addition to Nrf2/ARE/HO-1 signaling pathway, to exert its cytoprotective effect.…”

Section: Discussionsupporting

confidence: 92%

20C, a bibenzyl compound isolated from Gastrodia elata, protects PC12 cells against rotenone-induced apoptosis via activation of the Nrf2/ARE/HO-1 signaling pathway

et al. 2016

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Section: Discussionsupporting

confidence: 92%

20C, a bibenzyl compound isolated from Gastrodia elata, protects PC12 cells against rotenone-induced apoptosis via activation of the Nrf2/ARE/HO-1 signaling pathway

et al. 2016

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“…In physiological conditions, p38 MAPK is at a static status by binding with its inhibitor, Keap1 14. Various pathological stimuli and intracellular responses activate p38 MAPK by phosphorylation, which facilitates disassociation of Nrf2/Keap1 complex and nuclear translocation of Nrf2 38.…”

Section: Discussionmentioning

confidence: 99%

“…The activation of Nrf2/ARE signaling is regulated by the upstream kinases. Theses kinases are referred to as mitogen‐activated protein kinases (MAPKs) 14. As a member of the MAPKs family, p38 MAPK is a key signaling transducer in response to harmful stimuli, including ROS 15.…”

Section: Introductionmentioning

confidence: 99%

Matrine‐Type Alkaloids Inhibit Advanced Glycation End Products Induced Reactive Oxygen Species‐Mediated Apoptosis of Aortic Endothelial Cells In Vivo and In Vitro by Targeting MKK3 and p38MAPK Signaling

Liu

Zhang

et al. 2017

JAHA

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BackgroundThe matrine‐type alkaloids are bioactive components extracted from Sophora flavescens, which is used in treatment of diabetes mellitus in traditional Chinese medicine. Advanced glycation end products mediate diabetic vascular complications. This study was aimed to investigate the protective effects and molecular mechanisms of matrine‐type alkaloids on advanced glycation end products–induced reactive oxygen species–mediated endothelial apoptosis.Methods and ResultsRats aorta and cultured rat aortic endothelial cells were exposed to advanced glycation end products. Matrine‐type alkaloids, p38 mitogen‐activated protein kinase (MAPK) inhibitor, and small interference RNAs against p38 MAPK kinases MAPK kinase kinase (MKK)3 and MKK6 were administrated. Intracellular reactive oxygen species production, cell apoptosis, phosphorylation of MKKs/p38 MAPK, and expression levels of heme oxygenase/NADPH quinone oxidoreductase were assessed. The nuclear factor erythroid 2‐related factor 2 nuclear translocation and the binding activity of nuclear factor erythroid 2‐related factor 2 with antioxidant response element were also evaluated. Matrine‐type alkaloids suppressed intracellular reactive oxygen species production and inhibited endothelial cell apoptosis in vivo and in vitro by recovering phosphorylation of MKK3/6 and p38 MAPK, nuclear factor erythroid 2‐related factor 2 nuclear translocation, and antioxidant response element binding activity, as well as the expression levels of heme oxygenase/NADPH quinone oxidoreductase. p38 MAPK inhibitor treatment impaired the effects of matrine‐type alkaloids in vivo and in vitro. MKK3/6 silencing impaired the effects of matrine‐type alkaloids in vitro.ConclusionsMatrine‐type alkaloids exert endothelial protective effects against advanced glycation end products induced reactive oxygen species–mediated apoptosis by targeting MKK3/6 and enhancing their phosphorylation.

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“…Therefore, EEEP, EEBGP and EECP could stimulate Nrf2 to translocate into the nucleus to bind to DNA to regulate the expression of HO-1, GCLM and TrxR1. Moreover, numerous studies have confirmed that the activation of p38 and Erk kinases was required to activate Nrf2 in regulating the expression of antioxidant genes (HO-1, TrxR1 and GCLM) (Zipper & Mulcahy 2000Jiang et al 2014). Thus, we inferred that EEBGP regulated the expression of HO-1, TrxR1 To determine the inhibition effects of the inhibitors on p38 and Erk, RAW264.7 cells were pre-treated with SB203580 or PD98059 for 0.5 h and then cultured in the presence or absence of EEEP, EEBGP and EECP for 1 h. (F-I) To confirm that the expression of HO-1, GCLM and TrxR1 was regulated by p38/p-p38 kinase or Erk/p-Erk kinase, RAW264.7 cells were pre-treated with SB203580 or PD98059 for 0.5 h and cultured in the presence or absence of EEEP, EEBGP and EECP for 5 h, then the medium was removed, and the cells were cultured with new medium for an additional 4 h. All the genes expressed at protein level were detected by western blot and -tubulin was used as an internal reference.…”

Section: The Effects Of Eeep Eebgp and Eecp On The Nuclear Translocamentioning

confidence: 99%

Antioxidant activities and molecular mechanisms of the ethanol extracts ofBaccharispropolis andEucalyptuspropolis in RAW64.7 cells

Zhang

Shen

Wang

et al. 2016

Pharmaceutical Biology

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Context Numerous studies have reported that propolis possesses strong antioxidant activities. However, their antioxidant molecular mechanisms are unclear. Objective We utilize ethanol extracts of Chinese propolis (EECP) as a reference to compare ethanol extracts of Eucalyptus propolis (EEEP) with ethanol extracts of Baccharis propolis (EEBGP) based on their antioxidant capacities and underlying molecular mechanisms. Materials and methods HPLC and chemical analysis are utilized to evaluate compositions and antioxidant activities. ROS-eliminating effects of EEBGP (20-75 mg/mL), EEEP (1.25-3.75 mg/mL) and EECP (1.25-5 mg/mL) are also determined by flow cytometry analysis. Moreover, we compared antioxidant capacities by determining their effects on expressions of antioxidant genes in RAW264.7 cells with qRT-PCR, western blot and confocal microscopy analysis. Results EEBGP mainly contains chlorogenic acid (8.98 ± 0.86 mg/g), kaempferide (11.18 ± 8.31 mg/g) and artepillin C (107.70 ± 10.86 mg/g), but EEEP contains 10 compositions, whereas EECP contains 17 compositions. Meantime, although EEEP shows DPPH (IC 50 19.55 ± 1.28), ABTS (IC 50 20.0 ± 0.31) and reducing power (2.70 ± 0.08 mmol TE/g) better than EEBGP's DPPH (IC 50 43.85 ± 0.54), ABTS (IC 50 38.2 ± 0.33) and reducing power (1.53 ± 0.05 mmol TE/g), EEBGP exerts much higher ROS inhibition rate (40%) than EEEP (under 20%).

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Gastrodin protects against MPP+-induced oxidative stress by up regulates heme oxygenase-1 expression through p38 MAPK/Nrf2 pathway in human dopaminergic cells

Cited by 103 publications

References 36 publications

20C, a bibenzyl compound isolated from Gastrodia elata, protects PC12 cells against rotenone-induced apoptosis via activation of the Nrf2/ARE/HO-1 signaling pathway

20C, a bibenzyl compound isolated from Gastrodia elata, protects PC12 cells against rotenone-induced apoptosis via activation of the Nrf2/ARE/HO-1 signaling pathway

Matrine‐Type Alkaloids Inhibit Advanced Glycation End Products Induced Reactive Oxygen Species‐Mediated Apoptosis of Aortic Endothelial Cells In Vivo and In Vitro by Targeting MKK3 and p38MAPK Signaling

Antioxidant activities and molecular mechanisms of the ethanol extracts ofBaccharispropolis andEucalyptuspropolis in RAW64.7 cells

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