Gastroentérites aiguës à Vibrio parahaemolyticus à Dakar

Soumare, M.; Seydi, M; Gbabangai, E.; Diop, Sylvie Audrey; Ndour, C.T.; Sow, A.I.; Diop, Bernard Marcel; Sow, Papa Salif

doi:10.1016/j.medmal.2007.07.002

Cited by 2 publications

(1 citation statement)

References 5 publications

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“…Furthermore, V . parahaemolyticus is also an important foodborne pathogen in humans, which could cause the acute gastroenteritis with diarrhoeal by the consumption of contaminated raw seafood (Soumaré et al., 2007 ). Therefore, the contamination of V .…”

Section: Introductionmentioning

confidence: 99%

Vibrio parahaemolyticus CadC regulates acid tolerance response to enhance bacterial motility and cytotoxicity

Wang

et al. 2021

Journal of Fish Diseases

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Pathogens adapted to sub‐lethal acidic conditions could increase the virulence and survival ability under lethal conditions. In the aquaculture industry, feed acidifiers have been used to increase the growth of aquatic animals. However, there is limited study on the effects of acidic condition on the virulence and survival of pathogens in aquaculture. In this study, we investigated the survival ability of Vibrio parahaemolyticus at lethal acidic pH (4.0) after adapted the bacteria to sub‐lethal acidic pH (5.5) for 1 hr. Our results indicated that the adapted strain increased the survival ability at lethal acidic pH invoked by an inorganic (HCl) or organic (citric) acid. RNA‐sequencing (RNA‐seq) results revealed that 321 genes were differentially expressed at the sub‐lethal acidic pH including cadC, cadBA and groES/groEL relating to acid tolerance response (ATR), as well as genes relating to outer membrane, heat‐shock proteins, phosphotransferase system and flagella system. Quantitative real‐time polymerase chain reaction (qRT‐PCR) confirmed that cadC and cadBA were upregulated under sub‐lethal acidic conditions. The CadC protein could directly regulate the expression of cadBA to modulate the ATR in V. parahaemolyticus. RNA‐seq data also indicated that 113 genes in the CadC‐dependent way and 208 genes in the CadC‐independent way were differentially expressed, which were related to the regulation of ATR. Finally, the motility and cytotoxicity of the sub‐lethal acidic adapted wild type (WT) were significantly increased compared with the unadapted strain. Our results demonstrated that the dietary acidifiers may increase the virulence and survival of V. parahaemolyticus in aquaculture.

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Section: Introductionmentioning

confidence: 99%

Vibrio parahaemolyticus CadC regulates acid tolerance response to enhance bacterial motility and cytotoxicity

Wang

et al. 2021

Journal of Fish Diseases

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Vibrio choleraeandVibrio parahaemolyticusDetected in Seafood Products from Senegal

Coly¹,

Sow

Seydi

et al. 2013

Foodborne Pathogens and Disease

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The detection of pathogenic Vibrio in seafood from Senegal has generated five food alerts in the European Union. To investigate the presence and abundance Vibrio cholerae and Vibrio parahaemolyticus in seafood and coastal and estuarine waters, 123 seafood samples and 52 water samples were collected during 2007-2009 from two large seafood markets in Dakar, and from different oceanic and estuarine areas of the country. V. parahaemolyticus was detected in 30.1% of seafood samples, whereas presence of V. cholerae was only found in 1.6%. In water samples, V. parahaemolyticus and V. cholerae were detected in 28.8% and 5.7% of the samples, respectively. Abundance of V. parahaemolyticus in seafood from the fishing areas ranged from <0.3 to 7.5 most probable number (MPN) per gram. In samples from markets, densities of V. parahaemolyticus showed higher values ranging from 0.61 to >110 MPN/g. Densities of V. cholerae in the two positive seafood samples reached values of 0.36 and 0.61 MPN/g, repectively. V. parahaemolyticus strains were found to possess tlh, but not tdh and trh by polymerase chain reaction, and all the strains of V. cholerae were non-O1 or non-O139. These results suggest that the prevalence of high salinities in coastal and estuarine environments of Senegal limits the occurrence of V. parahaemolyticus and V. cholerae, despite warmer temperatures prevailing in seawater environments throughout the year. Furthermore, temperature abuse driven by a deficient cold chain over the distribution and retail sales may represent a major risk due to the postharvest multiplication of these Vibrio pathogens.

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Gastroentérites aiguës à Vibrio parahaemolyticus à Dakar

Cited by 2 publications

References 5 publications

Vibrio parahaemolyticus CadC regulates acid tolerance response to enhance bacterial motility and cytotoxicity

Vibrio parahaemolyticus CadC regulates acid tolerance response to enhance bacterial motility and cytotoxicity

Vibrio choleraeandVibrio parahaemolyticusDetected in Seafood Products from Senegal

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