Gastrointestinal cancers reactive for the PAb416 antibody against JCV/SV40 T-Ag lack JCV DNA sequences while showing a distinctive pathologic profile

Abstract

Our immunohistochemical and molecular results do not support the idea that JCV T-Ag has a role in gastrointestinal carcinogenesis. It is possible that PAb416, besides binding the viral protein, may cross-react with a hitherto undefined protein whose expression is associated with a distinct pathological profile and, at least in gastric cancers, with worse prognosis.

CIITA-related block of HLA class II expression, upregulation of HLA class I, and heterogeneous expression of immune checkpoints in hepatocarcinomas: implications for new therapeutic approaches

“…Immunohistochemical study was performed on 3 μm formalin-fixed, paraffin embedded sections deparaffinised and rehydrated through alcohol series to water, as previously described. 56 Briefly, endogenous activity was blocked with 3% aqueous hydrogen peroxide for 10 min, antigen retrieval was performed for each antigen in a domestic 750 KW microwave oven with different solutions (EDTA or citrate buffer pH 6,0) as detailed in Table 3. Primary antibodies were applied overnight at 4°C followed by a polymeric detection system (Ultravision DAB Detection System, LabVision, Värmdö Sweden) according to the manufacturer's protocol.…”

An Unusual Presentation of Metastatic BK Virus-Associated Urothelial Carcinoma Arising in the Allograft, Persisting After Transplant Nephrectomy

“…This supports the hypothesis that BK virus is playing a role in driving tumor cell division in these tumors, not merely indiscriminately infecting background cells that may randomly undergo tumorigenesis via an unrelated process. Although the utility of SV40 IHC for identifying viral involvement has been called into question by some, particularly 1 study examining gastrointestinal tumors, 17 SV40 (T-Ag) IHC positivity in the context of posttransplant urinary tract carcinomas in immunosuppressed patients is highly concordant with actual BK viral integration into the tumor genome. 14 …”

Biological Activities of Black Truffle (TerfeziaClaveryi) Against Dietary Acrylamide-Induced Toxicity in Rats Liver: Biochemical and Histopathological Study

“…Primary antibodies were applied overnight at 4°C according to the manufacturer's protocol. The immunoreaction was developed with 3.3ʹ-diaminobenzidine tetrahydrochloride (DAB) (Sigma Aldrich, St. Louis, MO, USA) as chromogen (Al-Rasheed et al, 2017;Chiaravalli et al, 2013).…”