Gastrointestinal Motility, Mucosal Mast Cell, and Intestinal Histology in Rats: Effect of Prednisone

Lima, Maysa Bruno de; Gama, Loyane Almeida; Hauschildt, Andrieli Taise; Dall'Agnol, Denize Jussara Rupolo; Corá, Luciana Aparecida; Américo, Madileine Francely

doi:10.1155/2017/4637621

Cited by 8 publications

(4 citation statements)

References 48 publications

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“…A previous study by Lima et al (40) found that the treated rats orally with prednisolone decreased villus height, whereas crypt depth, longitudinal and circular muscles were not affected. These findings prove a reduction of intestinal absorption, which may be connected with symptoms and diverse gastrointestinal dysfunctions.…”

Section: Discussionmentioning

confidence: 88%

Evaluation of histological changes induced by prednisolone and cyclophosphamide in some organs of male albino mice

AL-Sharqi

Chaloob

Al-Saleem

2022

IJVS

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Prednisolone is a synthetic corticosteroid used to treat various diseases. It is known to be used to treat many conditions such as autoimmune diseases and asthma. Cyclophosphamide is a type of nitrogen mustard therapy that works by alkylation of DNA and is used as an immunosuppressant in rheumatoid arthritis and the treatment of many cancers as well. Due to the wide use of these two drugs, the study aimed to evaluate the histological changes in the liver, kidneys, and small intestine of mice. Seventy-five adult mice aged 8-12 weeks were used which were divided into three groups, the first group was orally dosed with 0.1 mg/kg prednisolone, the second group was orally dosed with 0.1 mg/kg cyclophosphamide, and the third group received orally distilled water for 30 days daily. After 24 hours of the last treatment, the animals were sacrificed and the organs (liver, kidney, small intestine) were taken out and placed in 10% formalin solution until histological techniques were performed. The results of the study showed a statically significant difference at P>0.05 of histological changes in the studied organs represented by necrosis, fibrosis, cell degeneration, congestion, and hemorrhage of blood vessels and inflammatory cells when compared with the control group, and that the highest significant difference for these changes was at grade 1 and 2. Our study confirms that these drugs cause histological changes that differed in severity between organs as well as within a single organ when compared to the control group and that cyclophosphamide causes more histological changes than prednisolone.

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Section: Discussionmentioning

confidence: 88%

Evaluation of histological changes induced by prednisolone and cyclophosphamide in some organs of male albino mice

AL-Sharqi

Chaloob

Al-Saleem

2022

IJVS

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“…The default rat fasted physiology with theoretical surface area/volume was used with a modified mean stomach transit time set to 1.5 h to reflect data from the literature. 17 The drug properties and pharmacokinetic parameters used in the itraconazole absorption model are summarized in Table 1. The inputs for the calculation of itraconazole P eff,nano are summarized in Table 2.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The itraconazole absorption model was set up to evaluate the oral absorption of the 25% itraconazole:Affinisol 716 ASD that formed nanoparticles and Sporanox, which did not form nanoparticles, using the nanomodified permeability method. The default rat fasted physiology with theoretical surface area/volume was used with a modified mean stomach transit time set to 1.5 h to reflect data from the literature . The drug properties and pharmacokinetic parameters used in the itraconazole absorption model are summarized in Table .…”

Section: Resultsmentioning

confidence: 99%

Practical Approach to Modeling the Impact of Amorphous Drug Nanoparticles on the Oral Absorption of Poorly Soluble Drugs

Stewart

Graß

2019

Mol. Pharmaceutics

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Recently published studies have proposed that amorphous drug nanoparticles in gastrointestinal fluids may be beneficial for the absorption of poorly soluble compounds. Nanosized drug particles are known to provide rapid dissolution rates and, in some instances, a slight increase in solubility. However, in recent studies, the differences observed in vivo could not be explained solely by these attributes. Given the high dose and very low aqueous solubility of the study compounds, rapid equilibration to the drug-saturated solubility in gastrointestinal fluid would occur independent of the presence of nanoparticles. Alternatively, it has been proposed that drug nanoparticles (ca. ≤ 200 to 300 nm) may provide a “shuttle” for drug across the unstirred water layer (UWL) adjacent to the intestinal epithelium, particularly for low solubility/lipophilic compounds where absorption may be largely UWL-limited. This transport mechanism would result in a higher unbound drug concentration at the surface of the epithelium for absorption. This study evaluates this mechanism using a simple modification of the effective permeability to account for the effect of drug nanoparticles diffusing across the UWL. The modification can be made using inputs for solubility and nanoparticle size. The permeability modification was evaluated using three published case studies for amorphous formulations of itraconazole, anacetrapib, and enzalutamide, where the formation of amorphous drug nanoparticles upon dissolution resulted in improved drug absorption. Absorption modeling was performed using GastroPlus to assess the impact of the nanomodified permeability method on the accuracy of model prediction compared to in vivo data. Simulation results were compared to those for baseline simulations using an unmodified effective permeability. The results show good agreement using the nanomodified permeability, which described the data better than the standard baseline predictions. The nanomodified permeability method can be a suitable, fit-for-purpose in silico approach for evaluating or predicting oral absorption of poorly soluble, UWL-limited drugs from formulations that produce a significant number of amorphous drug nanoparticles.

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“…DEX also alters the liver morphology according to the previous report (Sultana et al, 2020b). The effects of prednisolone related to gastrointestinal motility, intestinal histology, and mucosal mast cells were studied in rats (Lima et al, 2017).…”

Section: Introductionmentioning

confidence: 97%

Evaluation of the effects of steroid growth promoter on the intestinal morphology and morphometry in broiler chicken.

Islam

Sultana

Das

et al. 2022

Preprint

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The intestine of poultry plays a significant role in the health and production through enzymatic and microbial digestion of feed as well as absorption of dietary nutrients. The current study was designed to explore the gross and histological alterations in the broiler duodenum and cecum triggered by dietary dexamethasone (DEX). The study was conducted on four homogenous groups of one-day-old (20 chicks/group) i.e. one control Non-DEX and three treatment groups (DEX-1, DEX-2, and DEX-3). The broilers were fed commercial broiler feed containing DEX at the rate of 0, 3, 5, and 7mg/kg feed in the Non-DEX, DEX-1, DEX-2, and DEX-3 groups respectively. The gross morphologic and morphometric data were recorded immediately after the collection of samples on days 7, 14, 21, and 28. Then, the tissue samples were processed for histological investigation. In the gross morphometric study, the weight, length, and width of the intestine were found significantly less in the DEX groups. Histopathological study results showed degeneration of intestinal glands (duodenum), mucosa, and lymphatic nodules with loss of lymphatic nodules (cecum). The percentage of the degenerated nodule was also decreased. The length, width, and surface area of the duodenal villi, thickness of the mucosal layer of the cecum, and diameter of the cecal lymphatic nodules were significantly decreased in all the DEX groups. The magnitude of the alterations was associated with both the dose and duration of DEX treatment. However, the current study results imply that DEX treatment significantly alters the morphologic and morphometric characteristics of the broiler intestine.

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Gastrointestinal Motility, Mucosal Mast Cell, and Intestinal Histology in Rats: Effect of Prednisone

Cited by 8 publications

References 48 publications

Evaluation of histological changes induced by prednisolone and cyclophosphamide in some organs of male albino mice

Evaluation of histological changes induced by prednisolone and cyclophosphamide in some organs of male albino mice

Practical Approach to Modeling the Impact of Amorphous Drug Nanoparticles on the Oral Absorption of Poorly Soluble Drugs

Evaluation of the effects of steroid growth promoter on the intestinal morphology and morphometry in broiler chicken.

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