Gastroprotective effect of Astragaloside IV: role of prostaglandins, sulfhydryls and nitric oxide

Abstract

This investigation evaluated the gastroprotective activity of Astragaloside IV, a cycloartane-type triterpene glycoside isolated from Astragalus zahlbruckneri. Gastric mucosal damage was induced in rats by intragastric ethanol (1 mL/rat). Rats treated orally with Astragaloside IV suspended in Tween 80 at 3, 10 and 30 mg kg(-1), showed 15, 37 and 52% gastroprotection, respectively. The gastroprotection observed at 30 mg kg(-1) for this compound was attenuated in rats pretreated with N(G)-nitro-L arginine methyl ester (70 mg kg(-1), i.p), a nitric oxide (NO)-synthase inhibitor, suggesting that the gastroprotective mechanism of this glycoside involves, at least in part, the participation of NO. The gastroprotective effect of Astragaloside IV was not affected by the inhibition of prostaglandin synthesis with indometacin (10 mg kg(-1), s.c.) nor by the block of endogenous sulfhydryls with N-ethylmaleimide (NEM, 10 mg kg(-1), s.c.). Carbenoxolone was used as a gastroprotective model drug and showed a dose-dependent gastroprotective effect (25, 43 and 88% of gastroprotection, at 3, 10 and 30 mg kg(-1), respectively). The partial participation of prostaglandins, sulfhydryls and NO was observed in the gastroprotective mechanism of carbenoxolone.

Chemical comparison and classification of Radix Astragali by determination of isoflavonoids and astragalosides

“…The categorized constituents G1, G2, and G5 represented compounds related to calycosin, ononin and astragalosides. These components showed major aspects of biological activities of RA [24][25][26][27][28][29][30][31][32][33], suggesting that it is practical to use the categorized constituents for quality assessment. The highest contents of G1, G2 and total isoflavonoids were found in samples from Heilongjiang.…”

Effects of Astragaloside IV on Action Potentials and Ionic Currents in Guinea-Pig Ventricular Myocytes

“…antioxidation, [3][4][5] antivirus, 6,7) anti-apoptosis, 8,9) anti-hyperglycemic, 10,11) neuroprotection, 13) gastroprotection, 14) anti-chronicasthma, 15) and anti-lung-cancer. 16) One of AS-IV's targets is the cardiovascular system, and especially the heart.…”

Carbenoxolone gastroprotective mechanism: participation of nitric oxide/cGMP/KATP pathway in ethanol-induced gastric injury in the rat

“…To investigate the involvement of endogenous NO in the gastroprotective effect of carbenoxolone, l ‐NAME (70 mg/kg dissolved in saline solution) was intraperitoneally administered 30 min before carbenoxolone (30 mg/kg, p.o. ); 30 min after carbenoxolone administration, the gastric mucosal lesions were induced and measured as described [14].…”