Gastroprotective effects of goniothalamin against ethanol and indomethacin-induced gastric lesions in rats: Role of prostaglandins, nitric oxide and sulfhydryl compounds

Vendramini–Costa, Débora Barbosa; Monteiro, Karin Maia; Iwamoto, Leilane Hespporte; Jorge, Michelle Pedroza; Tinti, Sirlene Valério; Pilli, Ronaldo A.; Carvalho, João Ernesto de

doi:10.1016/j.cbi.2014.10.025

Cited by 22 publications

(23 citation statements)

References 42 publications

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“…The control animals (T8, T12, T22) were treated (gavage) with carboxymethyl cellulose (CMC) 0.05% or phosphate-buffered saline (PBS) + 1% Tween 80 (10 mL/kg) vehicles (n = 40). The T1GTN and T2GTN groups both received 150 mg/kg GTN (gavage) (modified from Vendramini-Costa et al 2014), three times a week for 30 days only (in 8 to 12-week-old mice) and all the animals in both groups were killed at different ages (T1GTN at 12 weeks of age and T2GTN at 22 weeks of age) ( Supplementary Fig. 1, see section on supplementary data given at the end of this article).…”

Section: Animalsmentioning

confidence: 99%

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Anti-inflammatory therapies in TRAMP mice: delay in PCa progression

Kido¹,

Montico²,

Sauce³

et al. 2016

Endocrine-Related Cancer

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The aim of this study was to characterize the structural and molecular biology as well as evaluate the immediate and late responses of prostatic cancer in the transgenic adenocarcinoma of the mouse prostate (TRAMP) model after treatment with goniothalamin (GTN) and celecoxib. The treated mice received GTN (150 mg/kg, gavage) or celecoxib (10 mg/kg, gavage) from 8 to 12 weeks of age. They were killed at different ages: the immediate-response groups at 12 weeks and the late-response groups at 22 weeks. The ventral prostate was collected for light microscopy, immunohistochemistry, western blotting, TUNEL, and ELISA. Morphological analyses indicated that GTN treatment delayed the progression of prostatic adenocarcinoma, leading to a significant decrease of prostatic lesion frequency in both experimental period responses to this treatment, mainly high-grade prostatic intraepithelial neoplasia and well-differentiated adenocarcinoma. Also, the celecoxib treatment showed a particular decrease in the proliferative processes (PCNA) in both the experimental periods. Despite celecoxib diminishing the COX2 and IGFR1 levels, GTN presented higher action spectrum considering the decrease of a greater molecular number involved in the proliferative and inflammatory processes in prostatic cancer. Goniothalamin attenuated the pro-inflammatory response in TRAMP prostatic microenvironment, delaying prostate cancer (PCa) progression. Celecoxib treatment was efficient in the regulation of COX2 in the TRAMP mice, mainly in the advanced disease grade. Finally, we concluded that inflammatory process control in early grades of PCa was crucial for the downregulation of the signaling pathways involved in the proliferative processes in advanced cancer grades.

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Section: Animalsmentioning

confidence: 99%

“…Goniothalamin was prepared according to a published procedure (de Fatima et al 2005) and it was emulsified with 1% Tween 80 (Synth, Diadema, SP, Brazil) and dissolved in PBS, pH 7.0 (Vendramini-Costa et al 2014). Celecoxib was obtained from CELEBRA (Pfizer Pharmaceuticals LLC, Caguas, Puerto Rico), and diluted in CMC 0.05% (Sozer et al 2011).…”

Section: Drugsmentioning

confidence: 99%

Anti-inflammatory therapies in TRAMP mice: delay in PCa progression

Kido¹,

Montico²,

Sauce³

et al. 2016

Endocrine-Related Cancer

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“…After 1 hour, the animals were euthanized by an overdose of thiopental and their stomachs were removed, by opening them along the greater curvature, and washed. After washing the stomachs with saline solution, the ulcerative lesion index (ULI) was scored as described by Vendramini-Costa et al 23 using the arbitrary scoring system shown in Table 1. Gastroprotection was calculated by comparing the treatment group with the negative control (saline solution) group, using the following formula:…”

Section: Ethanol-induced Gastric Ulcermentioning

confidence: 99%

Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity

Servat-Medina¹,

González-Gómez²,

Reyes-Ortega³

et al. 2015

IJN

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103

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Natural products using plants have received considerable attention because of their potential to treat various diseases. Arrabidaea chica (Humb. & Bonpl.) B. Verlot is a native tropical American vine with healing properties employed in folk medicine for wound healing, inflammation, and gastrointestinal colic. Applying nanotechnology to plant extracts has revealed an advantageous strategy for herbal drugs considering the numerous features that nanostructured systems offer, including solubility, bioavailability, and pharmacological activity enhancement. The present study reports the preparation and characterization of chitosan–sodium tripolyphosphate nanoparticles (NPs) charged with A. chica standardized extract (AcE). Particle size and zeta potential were measured using a Zetasizer Nano ZS. The NP morphological characteristics were observed using scanning electron microscopy. Our studies indicated that the chitosan/sodium tripolyphosphate mass ratio of 5 and volume ratio of 10 were found to be the best condition to achieve the lowest NP sizes, with an average hydrodynamic diameter of 150±13 nm and a zeta potential of +45±2 mV. Particle size decreased with AcE addition (60±10.2 nm), suggesting an interaction between the extract’s composition and polymers. The NP biocompatibility was evaluated using human skin fibroblasts. AcE-NP demonstrated capability of maintaining cell viability at the lowest concentrations tested, stimulating cell proliferation at higher concentrations. Antiulcerogenic activity of AcE-NP was also evaluated with an acute gastric ulcer experimental model induced by ethanol and indomethacin. NPs loaded with A. chica extract reduced the ulcerative lesion index using lower doses compared with the free extract, suggesting that extract encapsulation in chitosan NPs allowed for a dose reduction for a gastroprotective effect. The AcE encapsulation offers an approach for further application of the A. chica extract that could be considered a potential candidate for ulcer-healing pharmaceutical systems.

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“…Previous studies performed by our group showed that racemic GTN presents gastroprotective effects, anti-inflammatory activity and inhibits the development of Ehrlich tumor (mammary adenocarcinoma) in mice (19)(20)(21)(22). Recently, we showed that GTN induces apoptosis in HT-29 colon tumor cells, which was dependent on ROS generation and caspase activation (23).…”

Section: Introductionmentioning

confidence: 96%

Anti-inflammatory natural product goniothalamin reduces colitis-associated and sporadic colorectal tumorigenesis

Vendramini–Costa

Francescone

Posocco

et al. 2016

CARCIN

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The tumor microenvironment offers multiple targets for cancer therapy, including pro-tumorigenic inflammation. Natural compounds represent an enormous source of new anti-inflammatory and anticancer agents. We previously showed that the styryl lactone goniothalamin (GTN) has promising antiproliferative and anti-inflammatory activities. Because inflammation is a major driver of colorectal cancer (CRC), we therefore evaluated the therapeutic and preventive potentials of GTN in colitis, colitis-associated cancer (CAC) and spontaneous CRC. First, in a simplistic model of inflammation in vitro, GTN was able to inhibit cytokine production in bone marrow-derived macrophages induced by lipopolysaccharide. Next, in dextran sulfate sodium (DSS) induced-colitis model, mice treated with GTN displayed restored tissue architecture, increased cell proliferation in the colonic crypts and reduced epithelial damage. Moreover, colon tissue from GTN-treated mice had significantly less expression of the inflammatory genes interleukin 1β (IL-1β), tumor necrosis factor α (TNF-α), interleukin 6 (IL-6), S100A9, interleukin 23A (IL-23A), IL-22 and IL-17A. In the azoxymethane/DSS model of CAC, GTN reduced tumor multiplicity, load and size. Additionally, GTN suppressed production of IL-6, IL-17 and TNF-α in tumor tissue, as well as abrogated stromal immune cell activation and nuclear translocation of NF-κB. Finally, in a tamoxifen inducible model of sporadic CRC, GTN-treated mice had significantly fewer tumors and decreased levels of IL-17A, IL-6, S100A9 and TNF-α protein within the tumors. These results suggest that GTN possesses anti-inflammatory and antitumor activities and represents a preventive and therapeutic agent modulating the inflammatory environment in the colon during colitis as well as CAC and CRC development.

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Gastroprotective effects of goniothalamin against ethanol and indomethacin-induced gastric lesions in rats: Role of prostaglandins, nitric oxide and sulfhydryl compounds

Cited by 22 publications

References 42 publications

Anti-inflammatory therapies in TRAMP mice: delay in PCa progression

Anti-inflammatory therapies in TRAMP mice: delay in PCa progression

Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity

Anti-inflammatory natural product goniothalamin reduces colitis-associated and sporadic colorectal tumorigenesis

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Gastroprotective effects of goniothalamin against ethanol and indomethacin-induced gastric lesions in rats: Role of prostaglandins, nitric oxide and sulfhydryl compounds

Cited by 22 publications

References 42 publications

Anti-inflammatory therapies in TRAMP mice: delay in PCa progression

Anti-inflammatory therapies in TRAMP mice: delay in PCa progression

Chitosan&ndash;tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and&nbsp;antiulcerogenic activity

Anti-inflammatory natural product goniothalamin reduces colitis-associated and sporadic colorectal tumorigenesis

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Chitosan–tripolyphosphate nanoparticles as Arrabidaea chica standardized extract carrier: synthesis, characterization, biocompatibility, and antiulcerogenic activity