Gastroprotective Value of Berries: Evidences from Methanolic Extracts of Morus nigra and Rubus niveus Fruits

Abstract

This study evaluated the gastroprotective value of the methanol extracts from fruits of Morus nigra L. (black mulberry (MEMN)) and Rubus niveus Thunb (raspberry (MERN)). The total phenolic compounds and flavonoids were measured, as well as the in vitro 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenger activity. The gastroprotective effects of the extracts against 60% ethanol/0.3 M HCl were evaluated in mice. After that, the lipid hydroperoxides and reduced glutathione levels at ulcerated tissue were determined. The effects of extracts on H+/K+-ATPase activity were also verified. The extracts exhibited high contents of polyphenols; however, MERN presented 1.5-fold higher levels. The presence of flavonoids also was confirmed. In addition, MEMN (IC50 = 13.74 μg/mL) and MERN (IC50 = 14.97 μg/mL) scavenged DPPH radical. The MEMN reduced the ulcer area only at 300 mg/kg (p.o.) by 64.06%. Interestingly, MERN decreased the ulcer area in a superior potency (ED50 = 20.88 mg/kg), reducing the ulcer area by 81.86% at 300 mg/kg, and increased the gastric mucin levels. The antioxidant effects of extracts were evidenced by reduced lipoperoxides and increased reduction of glutathione amount in the gastric mucosa. However, MEMN or MERN did not change the H+/K+-ATPase activity. These results confirm that M. nigra and R. niveus are berries with a gastroprotective value by strengthening of gastric protective factors.

Protective effect ofMorus macrouraMiq. fruit extract against acetic acid-induced ulcerative colitis in rats: Involvement of miRNA-223 and TNFα/NFκB/NLRP3 inflammatory pathway

“…and Morus nigra, which are also rich in polyphenolic compounds and the results proved the gastroprotective effect of these extracts due to its anti-inflammatory effect (Chandra et al, 2016, Nesello et al, 2017. Taken together all the consistent previous findings with the current results, it was suggested that the protective effect of MFE against UC is most probably due to the presence of high content of phenolic compounds, which might have possessed a strong antiinflammatory effect.…”

“…Another study was performed using the mulberry big leaf extract of the same plant; Morus macroura and also proved its gastroprotective effect in a model of water immersion and restraint stress-induced gastric ulcer in mice (Wei et al, 2018). Furthermore, several studies were performed using the MFE prepared from other species such as Morus alba and Morus nigra , which are also rich in polyphenolic compounds and the results proved the gastroprotective effect of these extracts due to its anti-inflammatory effect (Chandra et al, 2016, Nesello et al, 2017). Taken together all the consistent previous findings with the current results, it was suggested that the protective effect of MFE against UC is most probably due to the presence of high content of phenolic compounds, which might have possessed a strong anti-inflammatory effect.…”

Corryocactus brevistylus (K. Schum. ex Vaupel) Britton & Rose (Cactaceae): Antioxidant, Gastroprotective Effects, and Metabolomic Profiling by Ultrahigh-Pressure Liquid Chromatography and Electrospray High Resolution Orbitrap Tandem Mass Spectrometry

“…The extracts from these species inhibited gastric lesions at 300 mg/kg by 64.06% and 81.86% respectively. The authors indicated that the studied berries are a source of antiulcer compounds and this may be related to their high polyphenol contents (Nesello et al, 2017). In our case, the inhibition displayed by CEX at 100 mg/kg, p.o.…”

Evaluation of antioxidant, antiinflammatory, and gastroprotective properties of Rubus fruticosus L. fruit juice

“…The molecular basis of these effects are the ability of these molecules to block MAPK pathways with the attendant activation of NF-κB, C/EBPδ, and AP-1 (Smeriglio et al, 2014). The antiulcerogenic activity of some isolated flavonoids like naringenin as well as the gastroprotective effects of berry extracts were already observed (Awaad, Al-Jaber, Moses, El-Meligy, & Zain, 2013;Chao et al, 2010;Nesello et al, 2017;Park, Kim, & Choi, 2012). The possible mechanism that could justify the observed antiinflammatory and gastroprotective effects of RFJ is the ability of some phenolic acids and flavonoids to inhibit both COX-2 and iNOS involved in the production of proinflammatory mediators, such as nitric oxide and prostaglandins, at the inflammatory site (Wu et al, 2016;Farzaei et al, 2015).…”