GC-MS ANALYSIS AND ANTIFUNGAL ACTIVITY OF METHANOLIC ROOT EXTRACT OF Chenopodium album AGAINST Sclerotium rolfsii

Abstract

Sclerotium rolfsii is a soil-borne fungal plant pathogen that causes diseases in more than 500 plant species. Chemical fungicides used to control this disease cause environmental pollution, therefore, plant derived compounds can be used as alternative to synthetic fungicides to reduce environmental pollution. Chenopodium album is a weed of family Chenopodiaceae that is used as food and also has medicinal importance. In the present study, antifungal activity of methanolic root extract of C. album was evaluated against S. rolfsii using six concentrations viz. 0.5, 1.0, 1.5, 2.0, 2.5 and 3.0 g 100 mL-1 amended in malt extract as growth medium. All the root extract concentrations significantly reduced fungal biomass by 15-58% over control. Gas chromatography-mass spectrometry (GC-MS) analysis of the methanolic root extract of C. album was performed. Six compounds were identified in methanolic root extract through GC-MS analysis. The most abundant compound was 1,2-benzenedicarboxylic acid, mono(2-ethylhexyl) ester (58.56%) followed by 9-octadecenoic acid (Z)-, methyl ester (12.75%) and 9-octadecenoic acid (Z)-, methyl ester (10.27%), which might be responsible for antifungal activity of methanolic root extract of C. album.

Evaluation of Antifungal Potential of Leaf Extract of Chenopodium murale Against Fusarium oxysporum f. sp. lycopersici

“…It belongs to family Chenopodiaceae. Members of this family are generally known for their antifungal activity against various fungal pathogens (Javaid and Amin, 2009;Arafat et al, 2015;Ali et al, 2017). In this study, efficacy of crude methanolic leaf extract of C. murale and its various organic fractions is reported for the management of F. oxysporum f. sp.…”

“…Higher concentrations (100 and 200 mg mL -1 ) almost completely arrested the fungal growth and limited its growth up to 96%, 98% and 94% in nhexane, chloroform and ethyl acetate fractions, respectively, over control (Figure 4). Previously, members of Chenopodiaceae are known to exhibit potent antimicrobial activity against many pathogens (Singh et al, 2011;Amjad and Alizad, 2012;Ali et al, 2017). Qasem and Abu-Blan (1995) reported that extracts of C. murale possess antifungal potential against Penicillium digitatum and Alternaria solani.…”

Management of Charcoal Rot of Mungbean by Two Trichoderma Species and Dry Biomass of Coronopus didymus

“…However, numerous fungicides available in the market are not only toxic but also pollute the environment (Anggriani et al, 2015) and create resistance in fungal pathogens (Kim et al, 2007). A likely alternative to resolve such problems is the use of natural resources, including microorganisms and products from higher plants (De Corato et al, 2014;Ali et al, 2017;Khurshid et al, 2018). Protecting plants against fungal pathogens using such alternatives may also reduce development of resistance in the pathogens (Trigui et al, 2013).…”

Identification of antifungal compounds from slender amaranth

“…After the completion of incubation at above mentioned specified conditions, filtration of fungal biomass was done on pre-weighted filter paper and this filtered material was dried in electric oven at 70 o C to obtain a dry fungal biomass. Similar procedure was applied to all fungal species for recording biomass of selected plant pathogenic fungal species (Ali et al, 2017).…”