Gc-MS Analysis and Extraction Optimization of Bioactive Compounds From Pelargonium Graveolens Lher Methanolic Extract and Their Activities as Pharmacological Agents

Abstract

Poly phenols are multifunctional secondary metabolites in plants. Less information is available concerning the statistical yield, optimal extraction method and pharmacological activity with regards to active compounds from Pelargonium graveolens LʼHér leaf. Therefore, Box-Behnken design was used to optimize extraction parameters such as the speed of agitation (rpm), the volume of methanol solvent (mL), time of extraction (h) and mass of plant material (g). A polynomial quadratic model produced satisfactory fitting of experimental data: % yield (Y): R 2 = 0.9943, total phenols (TPC): R 2 = 0.9621 and total flavonoids (TFC): R 2 = 0.9873 at p < 0.05. The antioxidant activities of optimized methanol extract and controls were evaluated by DPPH, ABTS scavenging, and ferric reducing power in vitro assays. The kinetics of the lipid protective activity was studied and fitted into a pseudosecond-order kinetic model. Total antioxidant capacity (TAC) of the extract and gallic acid showed donating potential of 0.35 ± 0.10 mg QE/g and 0.35 ± 0.10 mg QE/g, respectively. The best MIC value of 1.56 mg/mL was against E. coli. The cell proliferation and cytotoxic activities of the extracts were evaluated using the Cell Titer® blue cell viability and lactate dehydrogenase assay on HaCaT cells. Bioactive compounds present in the leaf of P. graveolens were identified by GC-MS analysis by methanol extract.

Environmentally Friendly Extraction of Bioactive Compounds from <i>Mentha arvensis</i> Using Deep Eutectic Solvent as Green Extraction Media

“…The phenolics and flavonoids extracted from M. arvensis using LC-MS with retention time and m/z are given in Table 5. The bioactive compounds play a significant role in the defensive system against various oxidative and microbial infections with no side effects [25]. Hydroxycinnamic acid derivatives (p-courmaric and caffeic acid) and flavonoids (chrysin, naringenin, phenylpyruvic acid, pinocembrin and hesperidin) have shown a wide range of various pharmacological activities such as antioxidant, antimicrobial and antiinflammation activities, and are thought to exert protective effects against major diseases such as cancer and cardiovascular diseases [26].…”

Kinetic modeling of phenolic compounds extraction from nutshells: influence of particle size, temperature and solvent ratio

“…The extraction of bioactive compounds from biomass, particularly phenolic compounds, has elicited scientific interest due to their numerous uses and benefits. For example, the health-promoting benefits derived from phenolic compounds, such as anti-proliferative, anti-inflammatory, antioxidant, and antimicrobial properties, among others, are well documented [1,2]. The two latter benefits, together with the coloring properties, among others, have been utilized in food preservation by the food and packaging industries as well as the cosmetic and textile industries [3].…”