GC-MS analysis, antibacterial, antioxidant study and brine shrimp lethality analysis of Trachyspermum ammi (L.) Sprague

Shova, D C; Maharjan, Binita; Shrestha, Timila; Bharati, Samjhana; Shrestha, Ram Lal

doi:10.3126/arj.v1i1.32452

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…Because aseptic procedures are not employed, it is a quick (24-hour), low-cost, and straightforward test. For 24 hours, brine shrimp nauplii were exposed to solutions containing varying percentages of chloroform extracts of A. calamus [11,23]. The figure of motile nauplii was used to determine the efficacy of the chloroform extracts.…”

Section: Brine Shrimp Lethality Assaymentioning

confidence: 99%

Chemical and biological analysis of extracts of Acorus calamus L.

Pokharel

Thapa

Karki

et al. 2023

J. Nepal Chem. Soc.

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The powdered plant materials of Acorus calamus were subjected to successive extraction using the cold percolation method with methanol, hexane, and chloroform solvent. Qualitative phytochemical analysis of methanol, hexane, and chloroform extracts showed the presence of alkaloids, saponins, glycosides, sterols, triterpenoids, and carbohydrates. Five different major compounds were identified by GC-MS analysis of the chloroform extract, with isoprothiolane (83.11%) being the most prevalent. The total phenolic content in the chloroform extract was calculated at 17.39 mg Gallic acid equivalent /g and the total flavonoid content was 3.37 mg quercetin equivalent/g of dry extract. The IC50 value of chloroform extract was found to be 576.19 μg/mL, and the LC50 value was found to be 66.21 μg/mL. Antibacterial activity was shown in Staphylococcus aureus in chloroform extract with a ZOI of 7 mm.

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Section: Brine Shrimp Lethality Assaymentioning

confidence: 99%

Chemical and biological analysis of extracts of Acorus calamus L.

Pokharel

Thapa

Karki

et al. 2023

J. Nepal Chem. Soc.

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Toxicity assessment and anti‐Vibrio activity of essential oils: Potential for application in shrimp aquaculture

Zheng

Bossier

2023

Reviews in Aquaculture

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This present paper aimed to review the past 4 years (2019–2022), the inhibition of Vibrio spp. (including Vibrio's causing AHPND) by EOs, as well as the potential toxicity of the EOs towards crustaceans, with an emphasis on Artemia spp. In the present review, 27 EOs from terrestrial plants are reported regarding their anti‐Vibrio activity. Among these 27 studies, Salvia officinalis and Thymus vulgaris (Lamiaceae family) were found to be the most numerous. Among the Vibrio spp., V. parahaemolyticus (non‐AHPND strain) was mostly researched. There are in total 68 publications about the toxicity of EOs in Artemia spp. Based on the four categories of toxicity towards Artemia (strongly toxic: LC50 < 100 μg/ml, moderately toxic: LC50:100–500 μg/ml, weakly toxic: LC50: 500–1000 μg/ml, and non‐toxic: LC50 > 1000 μg/ml), strong toxicity activity was found in 37 EOs, moderate toxicity in 15 EOs, weak activity for three EO plants and 13 non‐toxic extracts. In fact, LC50 values as low as 10.25 and 11.48 μg/ml were described in Artemisia vulgaris and Euryale ferox, respectively, showing these two plant EOs are strongly toxic to Artemia. Overall, and despite being generally considered “eco‐friendly and natural” products and safer than antibiotics, some of the EOs are toxic to target organisms. Thus, to establish an ecologically safe application of EOs in shrimp aquaculture, the correct use of these plant EOs (in terms of concentrations and duration) in aquaculture should be considered.

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GC-MS analysis, antibacterial, antioxidant study and brine shrimp lethality analysis of Trachyspermum ammi (L.) Sprague

Cited by 2 publications

References 11 publications

Chemical and biological analysis of extracts of Acorus calamus L.

Chemical and biological analysis of extracts of Acorus calamus L.

Toxicity assessment and anti‐Vibrio activity of essential oils: Potential for application in shrimp aquaculture

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