Gc-MS Analysis of Bioactive Compounds and Antimicrobial Activity of Cryptococcus Rajasthanensis Ky627764 Isolated From Bombyx Mori Gut Microflora.

Barretto, DeliciaAvilla; Vootla, ShyamKumar

doi:10.21474/ijar01/6700

Cited by 16 publications

(10 citation statements)

References 16 publications

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“…The GC-MS analysis of the bulb displayed alkane as the major group. In the 28 compounds revealed, the dominance of compounds such as 2-methyloctacosane with the peak area of 21.30% appeared at 27.601 min which is an alkane with antimicrobial [30]. Tetratetracontane with a peak area of 14.05% which appeared at 30.497 min is an alkane having antibacterial property [28].…”

Section: Discussionmentioning

confidence: 99%

Chemical constituents, antioxidant, and antimicrobial activity of Allium chinense G. Don

et al. 2020

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Background Allium chinense G. Don is a medicinal and aromatic herb belonging to the family Amaryllidaceae, characterized by a high saponin content. The previous report has mostly been focused on the bulb, and there is very limited work on the leaf. The information about biological and chemical constituent of A. chinense leaf is still inadequate in contrast to the investigations reported on the bulb. To the best of our knowledge, there is no report on the hexane extract of both bulb and leaf extract. Therefore, the present investigation was focused on identifying and characterization of the hexane extracts of A. chinense bulb and leaf quantitatively and by using the GC-MS method and to know its scavenging, antibacterial, and antifungal activity. Results Twenty-eight bioactive compounds were identified in the bulb and nine in the leaf extract by GC-MS analysis. The versatile compounds present in the bulb are 2-methyloctacosane (21.30%), tetracontane (14.05%), eicosane, 10-methyl (12.06%), heneicosane (8.46%), octadecyl trifluoroacetate (6.48%), and 1-heneicosanol (5.76%), whereas in the leaf, it was phytol (35.76%), tetratetracontane (18.49%), perhydrofarnesyl acetone (14.76%), and heptadecane, 2,6-dimethyl (10.79%). In quantitative estimation, saponins were estimated to have the highest with 375.000 ± 0.577 mg/g in the leaf and 163.750 ± 0.433 mg/g in the bulb. The DPPH antioxidant scavenging activity was found to be minimum in both the bulb (IC50 = 678.347 μg/ml) and leaf (IC50 = 533.337 μg/ml). A. chinense extracts of both leaf and bulb exerted potential antibacterial effects against Staphylococcus aureus and Pseudomonas aeruginosa. Leaf extract exhibited greater antifungal activity than the bulb against Aspergillus niger. Conclusion From the analysis, the hexane leaf extract exhibited higher antibacterial, antifungal, and antioxidant activity than the bulb. Their superior activity might be due to the higher content of total saponin and terpenes. This result will lead to further in-depth research towards the potential use of this plant; the bio-constituents can be further isolated and used in medical and therapeutic applications.

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Section: Discussionmentioning

confidence: 99%

Chemical constituents, antioxidant, and antimicrobial activity of Allium chinense G. Don

et al. 2020

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“…Hexacosane was identified in the Sanseveria liberica plant and has antibacterial activity (Rukaiyat et al, 2015). 2-methyloctacosane has antimicrobial properties against A. flavus, C. albicans, Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus (Barretto & Vootla, 2018). A study conducted by Ntalli et al (2016) reported that 2,4-decadienal, (E,E)-showed strong activity against Meloidogyne arenaria, Meloidogyne incognita and Meloidogyne javanica parasitic nematodes and promoted tomato growth.…”

Section: Comparison Of Volatile Metabolites Harvested In Winter and Summermentioning

confidence: 99%

“…have been reported. These volatile compounds are, moreover, bioactive and have been proven to have broad antimicrobial activity (Barretto & Vootla, 2018;Rukaiyat et al, 2015;Siyumbwa et al, 2019;Umaru et al, 2019).…”

Section: Comparison Of Volatile Metabolites Harvested In Winter and Summermentioning

confidence: 99%

Chemotaxonomic profiling of fungal endophytes of Solanum mauritianum (alien weed) using gas chromatography high resolution time-of-flight mass spectrometry (GC-HRTOF-MS)

2021

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Introduction Since ancient times medicinal plants have been used as medicine in many parts of the world to promote human health and longevity. In recent years many novel secondary metabolites of plants have been isolated and reported to provide lead compounds for new drug discoveries. Solanum mauritianum Scopoli is native to South America. It is reported to be used by native South Americans during famine as a vegetable and as medicine to cure various diseases. In South Africa the plant is viewed as weed and is facing eradication, however, this plant is a valuable subject for research into its potential pharmaceutical and chemical uses. This study elucidated the metabolic profile of fungal endophytes that have promising bioactive secondary metabolites against pathogenic microorganisms, including mycobacterium species. Material and methods Fungal endophytes from a weed Solanum mauritianum Scop. were used to synthesize secondary metabolites. Gas chromatograph high-resolution time-of-flight mass spectrometry (GC-HRTOF-MS) was used to analyse volatile compounds to prove that potentially fungal endophytes could be extracted from this weed. Extracts obtained with ethyl acetate were screened for phytochemicals and analyzed using a gas chromatograph high-resolution time-of-flight mass spectrometry system. Principal component analysis was used to compare the gas chromatograph high-resolution time-of-flight mass spectrometry data for differences/similarities in their clustering. Phytochemical screening was conducted on the crude extracts of fungal endophytes obtained from different parts of Solanum mauritianum Scopoli (leaves, ripe fruit, unripe fruit and stems). Results Phytochemical screening indicated the presents of alkaloids, flavonoids, glycosides, phenols, quinones and saponins. Quinones were not present in the crude extracts of Fusarium sp. A total of 991 compounds were observed in the fungal endophytes, and Cladosporium sp. (23.8%) had the highest number of compounds, compared to Paracamarosporium leucadendri (1.7%) and Talaromyces sp. (1.5%). Some volatile compounds such as eicosane, 2-pentadecanone, 2-methyloctacosane, hexacosane and tridecanoic acid methyl ester with antibacterial activity were also observed. Conclusion Compositional variations between the plant and fungal endophyte phytochemicals were observed. The results of this study indicate that fungal endophytes from Solanum mauritianum Scop. contain compounds that can be exploited for numerous pharmaceutical and medicinal applications.

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“…From fraction no. 7, eleven compounds were identified as di-butyl phthalate (11), 1-nonadecene, thieno [3,2-e] benzofuran (12), (1-decene) (13), di-isooctyl phthalate (14), bis (2-ethyl hexyl) phthalate ( 15), dodecane (16), eicosane (17), heneicosane (18), 1,2-benzenedi carboxylic acid (19), lauric acid (20), and dodecanoic acid (21). According to the previous literature, these compounds were isolated from other aforementioned studies.…”

Section: Gc-ms Analysis Of Volatile Componentsmentioning

confidence: 99%

“…Thereafter, air-dried powder samples of ethyl acetate crude extracts were processed by weighing and mixing them with silica powder (2 g) and dried at room temperature. This mixture was subjected to separation through column chromatography (CC) size exclusion of 2 × 40 cm containing 150 mg of the Sephadex LH 20 . A linear gradient of 100% chloroform was used for the starting gradient elution, and the polarity of methanol was gradually increased (100:0, 95:5, 90:10, 85:15, 80:20, 70:30, 60:40, 50:50, and 0:100 v:v) to 100% in 30 min.…”

Section: Purification Of Phacs By Column Chromatography and Thin-layer Chromatographymentioning

confidence: 99%

Enhanced Pharmaceutically Active Compounds Productivity from Streptomyces SUK 25: Optimization, Characterization, Mechanism and Techno-Economic Analysis

et al. 2021

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The present research aimed to enhance the pharmaceutically active compounds’ (PhACs’) productivity from Streptomyces SUK 25 in submerged fermentation using response surface methodology (RSM) as a tool for optimization. Besides, the characteristics and mechanism of PhACs against methicillin-resistant Staphylococcus aureus were determined. Further, the techno-economic analysis of PhACs production was estimated. The independent factors include the following: incubation time, pH, temperature, shaker rotation speed, the concentration of glucose, mannitol, and asparagine, although the responses were the dry weight of crude extracts, minimum inhibitory concentration, and inhibition zone and were determined by RSM. The PhACs were characterized using GC-MS and FTIR, while the mechanism of action was determined using gene ontology extracted from DNA microarray data. The results revealed that the best operating parameters for the dry mass crude extracts production were 8.20 mg/L, the minimum inhibitory concentrations (MIC) value was 8.00 µg/mL, and an inhibition zone of 17.60 mm was determined after 12 days, pH 7, temperature 28 °C, shaker rotation speed 120 rpm, 1 g glucose /L, 3 g mannitol/L, and 0.5 g asparagine/L with R2 coefficient value of 0.70. The GC-MS and FTIR spectra confirmed the presence of 21 PhACs, and several functional groups were detected. The gene ontology revealed that 485 genes were upregulated and nine genes were downregulated. The specific and annual operation cost of the production of PhACs was U.S. Dollar (U.S.D) 48.61 per 100 mg compared to U.S.D 164.3/100 mg of the market price, indicating that it is economically cheaper than that at the market price.

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Gc-MS Analysis of Bioactive Compounds and Antimicrobial Activity of Cryptococcus Rajasthanensis Ky627764 Isolated From Bombyx Mori Gut Microflora.

Cited by 16 publications

References 16 publications

Chemical constituents, antioxidant, and antimicrobial activity of Allium chinense G. Don

Chemical constituents, antioxidant, and antimicrobial activity of Allium chinense G. Don

Chemotaxonomic profiling of fungal endophytes of Solanum mauritianum (alien weed) using gas chromatography high resolution time-of-flight mass spectrometry (GC-HRTOF-MS)

Enhanced Pharmaceutically Active Compounds Productivity from Streptomyces SUK 25: Optimization, Characterization, Mechanism and Techno-Economic Analysis

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