GC-MS Analysis of Bioactive Compounds in Ethanol and Ethyl Acetate Fraction of Grapefruit (Citrus maxima L.) Rind

Abstract

The grapefruit (Citrus maxima L.) is a plant known by the public as a fruit consumed with various properties. This plant's use is well known, such as antioxidants, enhancing immunity, anti-aging, and antibacterial properties. This study aimed to identify and analyze the chemical compounds contained in C. maxima rind. The extract was obtained by the maceration method using ethanol and ethyl acetate as solvents. The fractionation process was carried out by Column Chromatography. Observation of thin-layer chromatography profiles with UV lamps 254 and 366 nm. Analysis of chemical compound components using GC-MS and data interpretation based on the Wiley 7.0 data library. The interpretation results of the EF1 fraction are β-copaen-4-α-ol; pentadecanoic acid; hexadecanoic acid; tetradecanoic acid; dotriacontane; osthol; 2H-1-benzopyran-2-one, 7-methoxy-8-(3-methyl-2-oxobutyl); furfural; 6-(2,3-Dihydroxy-3-methylbutyl)-7-methoxycoumarin; and 6-(iodomethyl)-5-methyl-4-oxahexanolide. The chemical compounds identified in EAF2 are 1-octadecanol; decane; tetracosane; hexacosane; and 1,2-benzenedicarboxylic acid (2-ethylhexyl) ester. It can be concluded that these compounds have biological and pharmacological activities.

Citrus maxima (Brum.) Merr. (Rutaceae): Bioactive Chemical Constituents and Pharmacological Activities

“…In addition to the compounds mentioned above, a few compounds like L-ascorbic acid, citric acid, decyl acetate, fumaric acid, hexanal, malonic acid, succinic acid, α- tocopherol, pentadecanoic acid, hexadecanoic acid, tetradecanoic acid have been isolated from fruit juice, peel, and leaves of C. maxima [ 28 , 79 ].…”