GC-MS Analysis of Bioactive Phytochemicals in Methanol Extract of Aerial Part and Callus of Dipterygium glaucum Decne

Choudhary, Dinesh; Jk, Shekhawat; Kataria, Vinod

doi:10.5530/pj.2019.11.165

Cited by 12 publications

(5 citation statements)

References 27 publications

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“…The chemical profiling of hexane fraction from S. biafrae plant revealed the presence of seventeen (17) while DMF had six (6) saturated and unsaturated fatty acids, phytol and benzene dicarboxylate esters. The most abundant saturated fatty acid identified is n-hexadecanoic acid (palmitic acid) known for exerting anti-cancerous, anti-inflammatory, antioxidant and anti-androgenic pharmacological effects [25]. This was followed by unsaturated fatty acid cis, cis, cis-9,12,15octadecatrienoic acid and phytol, an acyclic diterpene alcohol.…”

Section: Discussionmentioning

confidence: 99%

Metabolite Profiling and Cytotoxic Investigation ofSenecio biafraeOliv. & Hiern (Compositae) on Nauplii Brine Shrimps and Cancer Cell Lines

Akinola,

Oriyomi,

Ogundele

et al. 2023

Preprint

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The study investigated the cytoactivities of methanolic leaf extract (MLE) and fractions of Senecio biafrae, profiled, and elucidated their metabolites. Hydromethanolic extract of S. biafrae obtained by maceration was partitioned and subjected to cytotoxicity using brine shrimp lethality assay (BSLA), human cervical adenocarcinoma (HeLa) and prostate cancer cell (PC3) lines. The bioactive principles of most active fractions were profiled using GC-MS. The dichloromethane fraction (DMF) was purified using column and high-performance liquid chromatography to afford compounds 1 and 2 identified by 1H and 13C, DEPT-90, DEPT-135, COSY, NOESY and EIMS spectra. The MLE and its fractions exhibited good toxicity on nauplii brine shrimp. The HF exhibited a pronounced cytoactivity on PC-3 cell line with 92.8% followed by DMF. The GC-MS profiling of HF and DMF identified 17 and 6 constituents respectively comprising saturated and unsaturated fatty acids, diterpenoidal alcohol, phytol and aromatic compounds. Hexadecanoic acid, cis, cis, cis-9, 12, 15-octadecatrienoic acid, and phytol accounted for the highest percentage of the constituents in both fractions. Structure elucidation confirmed compounds 1 and 2 as stigmasterol and ergosterol with cytoactivity which compared favourably with doxorubicin. The study revealed that S.biafrae contains cytotoconstituents evidenced by proliferation inhibition of HeLa and PC-3 cell lines. The cyto-activities and bioconstituents indicate that S.biafrae has the potential to be considered as a candidate for drug development in the management of cancer-related conditions. Further investigation into the specific bioactive compounds and their mechanisms of action could provide valuable insights into the therapeutic potential of S.biafrae in cancer treatment.

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Section: Discussionmentioning

confidence: 99%

Metabolite Profiling and Cytotoxic Investigation ofSenecio biafraeOliv. & Hiern (Compositae) on Nauplii Brine Shrimps and Cancer Cell Lines

Akinola,

Oriyomi,

Ogundele

et al. 2023

Preprint

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“…(Saeida et al, 2014) 12 Ergost-5-en-3-ol,(3.beta. )-Anti-cancerous, hypocholesterolemic, Antioxidant (Choudhary et al,2019).…”

Section: Discussionmentioning

confidence: 99%

PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF ROOT EXTRACTS OF PARKIA BIGLANDULOSA (WIGHT&ARN.)

Yogashree¹,

P²,

H.C³

2021

PLANT ARCHIVES

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The current research work estimated to explore the qualitative and quantitative analysis of the major bioactive constituents of medicinally imperative plant Parkia biglandulosa in, the five solvent (petroleum ether, chloroform, ethyl acetate, methanolic and distilled water) root extracts of Parkia biglandulosa were predictable and characterized using GC-MS analysis. The explorations were accepted out in terms of five solvent extractions, intact extractive values, qualitative analysis, quantitative estimation and characterization of phytochemicals. The percentage value of yield extraction in petroleum ether extract was 34.74%, chloroform extract was 24.2%,ethyl acetate extract was 25%, methanol was 50% and distilled water was 54%. The preliminary phytochemical analysis showed the presence of alkaloids, flavonoids, terpenoids & phenolic compounds. The total metabolites content of five solvent extracts were compared with standards. The GC-MS analysis reveals the presence of various metabolites in three solvent(petroleum ether, ethyl acetate, methanol) extracts. It signifies that results revealed the presence of various bioactive metabolites which could be demoralized for their credible solicitations for therapeutic resolves.

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“…These six compounds were reported to have several biological activities. The n-Hexadecanoic acid which is the most abundant phytoconstituents present in hexane fraction had been reported to exert several biological activities including anti-cancerous, anti-inflammatory, antioxidant and anti-androgenic pharmacological properties 47 . This was followed by unsaturated fatty acid cis, cis, cis-9,12,15-Octadecatrienoic acid and phytol.…”

Section: Structure Elucidation Ofmentioning

confidence: 99%

Metabolite Profiling and Cytotoxic Investigation of Senecio biafrae Oliv. and Hiern (Compositae) on Nauplii Brine Shrimps and Cancer Cell Lines

Akinola,

Oriyomi,

Ogundele

et al. 2024

Asian J. Biol. Sci

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Background and Objective: Senecio biafrae possesses various therapeutic properties including hepatoprotective and antioxidant potentials. However, there is no information regarding its cytotoxic activity. Hence, this study investigated the cytoactivities of methanolic leaf extract (MLE) and fractions of Senecio biafrae, profiled and elucidated their metabolites. Materials and Methods: Hydromethanolic extract of S. biafrae obtained by maceration was partitioned and subjected to cytotoxicity screening using brine shrimp, human cervical adenocarcinoma (HeLa) and Prostate Cancer Cell (PC-3) lines. Bioactive principles of most of the active fractions were profiled using GC-MS. The dichloromethane (DCM) fraction was purified using column and High-Performance Liquid Chromatography (HPLC) to afford compounds 1 and 2 identified by 1 H and 13 C, DEPT-90, DEPT-135, COSY, NOESY and EI-MS spectra. Results: The MLE and its fractions exhibited significant toxicity on nauplii brine shrimp, while the hexane fraction exhibited a pronounced cytoactivity on PC-3 cell line with 92.8% followed by DCM fraction. The GC-MS profiling of hexane and DCM fractions identified 17 and 6 constituents respectively, comprising saturated and unsaturated fatty acids, diterpenoidal alcohol, phytol and aromatic compounds. Hexadecanoic acid, cis, cis, cis-9, 12, 15-octadecatrienoic acid and phytol accounted for the highest constituents in both fractions. Structure elucidation confirmed compounds 1 and 2 as stigmasterol and ergosterol with cytoactivity which compared favourably with doxorubicin. Conclusion: The study revealed that S. biafrae contains cytoconstituents evidenced by proliferation inhibition of HeLa and PC-3 cell lines. The cytoactivities and bioconstituents indicate that S. biafrae has potential to be considered as a candidate for drug development that can manage cancer-related conditions. Further investigation into the specific bioactive compounds and their mechanisms of action could provide valuable insights into the therapeutic potential of S. biafrae in cancer treatment.

show abstract

GC-MS Analysis of Bioactive Phytochemicals in Methanol Extract of Aerial Part and Callus of Dipterygium glaucum Decne

Cited by 12 publications

References 27 publications

Metabolite Profiling and Cytotoxic Investigation ofSenecio biafraeOliv. & Hiern (Compositae) on Nauplii Brine Shrimps and Cancer Cell Lines

Metabolite Profiling and Cytotoxic Investigation ofSenecio biafraeOliv. & Hiern (Compositae) on Nauplii Brine Shrimps and Cancer Cell Lines

PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF ROOT EXTRACTS OF PARKIA BIGLANDULOSA (WIGHT&ARN.)

Metabolite Profiling and Cytotoxic Investigation of Senecio biafrae Oliv. and Hiern (Compositae) on Nauplii Brine Shrimps and Cancer Cell Lines

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