GC/MS Analysis of Some Bioactive Constituents from Carthamus lanatus L.

Mitova, Maya I.; Taskova, Rilka M.; Попов, Симеон; Berger, Ralf G.; Krings, Ulrich; Handjieva, Nedjalka

doi:10.1515/znc-2003-9-1018

Cited by 23 publications

(15 citation statements)

References 2 publications

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“…Triterpenes attract attention because of their biological activity 15 . Squalene is a triterpene and has been reported in to have antimicrobial activity 16 .…”

Section: Discussionmentioning

confidence: 99%

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2012

IJPSR

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The Diethyl ether fraction of the Methanolic extract of Annona senegalensis (Pers) stem bark was prepared in yield of (0.16%). Preparative Thin Layer Chromatography analysis of the fraction resulted in the identification of two spots with antimicrobial activity. Subsequent GC/MS analysis of these spots resulted in the identification of ten components (spot one) and fourteen components (spot two). The fraction showed significant antimicrobial activity against clinical isolates of E. coli, S. enteriditis and S. dysenteriae.

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“…Triterpenes attract attention because of their biological activity 15 . Squalene is a triterpene and has been reported in to have antimicrobial activity 16 .…”

Section: Discussionmentioning

confidence: 99%

Untitled

2012

IJPSR

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“…Previously, Mitova et al . [11] isolated dehydroabietylic acid from the aerial part of C. lanatus and dehydroabietylamine from the Rosin plant. [3839] In the present investigation, we have isolated dehydroabietylamine from the leaves of C. tinctorius .…”

Section: Discussionmentioning

confidence: 99%

“…[6] Phytochemical studies of the species revealed the presence of sesquiterpene glycosides,[7] aromatic acids and serotonins,[8] flavonoids[910] and sterols and triterpenes. [11] The pharmacological properties of safflower have been evaluated for antitumor, sedative,[12] antimicrobial,[13] antiinflammatory and analgesic effects. [14] Previously, Lee et al .…”

Section: Introductionmentioning

confidence: 99%

Hepatoprotective and in vitro antioxidant effect of Carthamus tinctorious L, var Annigeri-2-, an oil-yielding crop, against CCl₄-induced liver injury in rats

Mahadevappa

Ck²,

Krishna

et al. 2011

Phcog Mag

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Background:The present investigation evaluates the hepatoprotective and in vitro antioxidant effect of methanolic extract and its isolated constituent, dehydroabietylamine, in Carthamus tinctorious L, var Annigeri-2-, an oil yielding crop.Materials and Methods:The hepatoprotective effects were estimated for the parameters viz, total bilirubin, total protein, serum alanine amino transaminase (ALT) and serum aspartate aminotransferase (AST) and alkaline phosphatase (ALP) and along with the pathological findings of hepatotoxicity. The in vitro antioxidant activity was evaluated by using free radical scavenging assays: DPPH, nitric oxide radical scavenging, hydroxyl radical, reducing power, ferrous ion chelating ability and total antioxidant capacity.Results:Both the methanolic extract (at 150 and 300 mg/kg bw) and dehydroabietylamine (at 50 mg/kg bw) showed significant liver protection against CCl4 -induced liver damage that was comparable with the standard drug, silymarin (100 mg/kg bw), in reducing the elevated serum enzyme markers. The liver sections of the animals treated with dehydroabietylamine elicit a significant liver protection compared with the methanolic extract against CCl4 -induced liver damage. Further, both the methanolic extract and dehydroabietylamine exhibited a considerable and dose-dependent scavenging activity of DPPH, nitric oxide and hydroxyl radical. Similarly, in the reducing power assay, the results were very persuasive. In addition, the Fe2+ chelating activity and the total antioxidant assay established the antioxidant property of the methanolic extract and its isolated constituent. Among the two experimental samples, dehydroabietylamine proved to be more effective for the said parameters.Conclusion:The potent antioxidant and its correlative hepatoprotective activity of the methanolic extract and isolated constituent dehydroabietylamine is therefore attributed to its antioxidant and free radical scavenging activities.

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“…This metabolite was soluble in most organic solvents, such as methanol, ethanol, chloroform and ethyl acetate, but had poor solubility in water. Sitosterol and stigmasterol, the possible intermediates of stigmastan-3,5-diene, have been described as chemical defences against some bacterial and fungal pathogens (Kiprono et al 2000;El-Shazly et al 2002;Mitova et al 2003), and as inhibitors of DNA polymerase (Prakash Chaturvedula et al 2003;Li et al 2004). Additionally, Wang and Maas (1997), reported that strawberry roots resistant to Phytophthora fragariae have higher levels of sitosterol.…”

Section: Fractionsmentioning

confidence: 99%

Root extracts from Mexican avocado (Persea americana var. drymifolia) inhibit the mycelial growth of the oomycete Phytophthora cinnamomi

et al. 2009

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Crude root extracts from Mexican avocado trees (Persea americana) were screened for antioomycete activity against Phytophthora cinnamomi. Fortyeight accessions from Mexican avocado trees were selected with potential resistance to P. cinnamomi according to environmental and site descriptors. Crude root extracts from these accessions were obtained and tested in vitro against the oomycete P. cinnamomi. Seven crude root extracts inhibited mycelial growth (>50%) and only root extracts from accessions 765-01 and 773-01 showed 100% of inhibition. Extracts from accessions 765-01 and 773-01 were analysed by preparative thin-layer chromatography and six fractions were detected under UV light. In both extracts, fractions IV (R f 0.85) and V (R f 0.9) showed in vitro inhibition (100%) against mycelial growth of P. cinnamomi. Fraction V was subjected to GC-MS analysis and stigmastan-3,5-diene (C 29 H 48 ) was identified as the major compound. In vitro assay showed that stigmastan-3,5-diene (100 ppm) inhibited the mycelial growth of P. cinnamomi. The constitutive presence of this compound in avocado roots offers possibilities to identify and to select potentially resistant plants to P. cinnamomi.

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GC/MS Analysis of Some Bioactive Constituents from Carthamus lanatus L.

Cited by 23 publications

References 2 publications

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Hepatoprotective and in vitro antioxidant effect of Carthamus tinctorious L, var Annigeri-2-, an oil-yielding crop, against CCl₄-induced liver injury in rats

Root extracts from Mexican avocado (Persea americana var. drymifolia) inhibit the mycelial growth of the oomycete Phytophthora cinnamomi

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GC/MS Analysis of Some Bioactive Constituents from Carthamus lanatus L.

Cited by 23 publications

References 2 publications

Untitled

Untitled

Hepatoprotective and in vitro antioxidant effect of Carthamus tinctorious L, var Annigeri-2-, an oil-yielding crop, against CCl4-induced liver injury in rats

Root extracts from Mexican avocado (Persea americana var. drymifolia) inhibit the mycelial growth of the oomycete Phytophthora cinnamomi

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Hepatoprotective and in vitro antioxidant effect of Carthamus tinctorious L, var Annigeri-2-, an oil-yielding crop, against CCl₄-induced liver injury in rats