GC–MS analysis of volatile organic compounds from Bambara groundnut rhizobacteria and their antibacterial properties

Ajilogba, Caroline Fadeke

doi:10.1007/s11274-019-2660-7

Cited by 47 publications

(30 citation statements)

References 43 publications

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“…It is interesting to note that banana plant metabolome profile [2] Acidithiobacillus ferrooxidans also changed when subjected to pH stress [6] suggesting that membrane permeability was compromised under extreme acidity (pH 1.5). It was also found that Dunaliella salina cultivated under high NaCl stress (1.5M) demonstrated the increase of 9,12,15octadecatrienoic acid (Z,Z,Z) (C18:3 Δ9, 12,15 ) synthesis [7]. Similarly, in Chlamydomonas reinhardtii the synthesis of octadecatrienoic acid (C18:3) dan octadecadienoic acid (C18:2) also increased under NaCl stress [8].…”

Section: Introductionmentioning

confidence: 85%

Metabolome profile of osmotolerant rhizobacteria under osmotic stress

Surya¹,

Palupi²,

Yuwono³

et al. 2021

Advances in Biological Sciences Research

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An osmotolerant rhizobacteria (Enterococcus flavescens), isolated from the weed rhizosphere, has been subjected to osmotic stress in broth media supplemented with varied concentrations of glucose and sodium chloride (NaCl). The aims of this work was to evaluate the cells' response and obtain a comprehensive view of metabolites profiles under osmotic stress. Cells were grown in rich medium (Luria Bertani, LB) supplemented with NaCl at varying concentrations (0.5 M -1.8 M NaCl) and glucose (5% -20%), both as a single or double stress condition. Physiological response was observed by measuring rhizobacterial growth and analysing metabolite synthesised using GC-MS. The results of this study demonstrated that different concentration of salt and glucose resulted in different metabolite profiles. It was also observed that cells cultivated in LB + 1,6 M NaCl and LB + 1,8 M NaCl medium resulted in only two different compounds detected, i.e amide and fatty acid. Under double stress, however, concentration of hexadecanoic acid, hexadecenoic acid, proline and glycerin was found increased, while under single-salt and glucose stress, hexadecanoic acid and 9-octadecenoic acidmethyl ester were detected. Those two acid were absent from the cells' metabolites under double stress. The results of this study thus suggested that osmotic stress provokes different pathway of fatty acid metabolism. The metabolome profile of the rhizobacteria under stress condition may also reveal specific mechanism of microbial tolerance to osmotic stress under different condition. The possible mechanism and pathway of osmotic stress in microorganisms will be discussed.

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Section: Introductionmentioning

confidence: 85%

Metabolome profile of osmotolerant rhizobacteria under osmotic stress

Surya¹,

Palupi²,

Yuwono³

et al. 2021

Advances in Biological Sciences Research

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“…In addition, Palmitin, 1,2-di-, 2aminoethyl hydrogen phosphate was reported for its ability to interact with and enhances the antimicrobial activities of cationic surfactants (Joondan, et al, 2014). Other phytocompounds of the plant include 15-methyl Hexadecanoic acid methyl ester (7.71%) which has both antibacterial and antifungal activity (Chandrasekaran et al, 2011), 2,2-Dimethyl hexan-3-one (3.19%) which is an important volatile organic compound that has been isolated as part of major antimicrobially active compound responsible for the broad spectrum antimicrobial activity of the chloroform crude extract of the medicinal plant Datura metel (Hossain et al, 2013) and butanol extract of 3 Bacillus spp (Bacillus amyloliquefaciens, Bacillus thuringiensis and Bacillus sp), isolated from rhizoides of Bamfara nut, with strong antagonism against B. cereus, E. feacalis and Fusarium graminearium, all of which are known to cause infections in humans, animals and plants with high rates of mortalities (Ajilogba et al, 2019 However, despite its antimicrobial activity, it has been classified by the European Chemicals Agency (ECHA, 2017) as a potential human toxin that can cause eye, skin and gastrointestinal irritations. Its presence in Aloe vera stem therefore support the antimicrobial activity of the stem and potential health risks to humans.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Screening and Antimicrobial Activity of Aqueous Stem Extract of Aloe vera on Some Common Pathogenic Bacteria

Salisu¹,

S²

2019

UJMR

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Aloe vera is one of the most popular ethnomedicinal plants that is commonly used in treating many infectious diseases. However, the knowledge of the spectrum of antimicrobial activity of the plant and its bioactive components is still inadequate. In this study, we carried out an automated determination of phytochemical components in aqueous extract (AE) of Aloe vera whole stem by GC-MS (Gas chromatography-mass spectrometry), determined the in vitro antimicrobial activity of the extract on some common pathogenic bacteria which included Escherichia coli, Salmonella typhi, Enterococcus faecalis and Staphylococcus aureus by agar well diffusion method, and determined the minimum inhibitory concentrations (MICs) and minimum Bactericidal concentration (MBCs) by agar dilution method. The result showed that the extract has strong antimicrobial activity against all the test organisms. No resistance was observed. The range for zones of inhibition, MIC and MBC were 12.33 ± 0.33-28.67 ± 0.60mm, 25mg/ml-50mg/ml and 50mg/ml-100mg/ml respectively. The most susceptible isolate was S. typhi (MBC = 50mg/ml) and the least sensitive isolate was E. faecalis (MBC = 150mg/ml). The GC-MS of the extract revealed 9 different compounds of which 82.82% are Palmitic acid methyl ester, (7Z)-7-Tetradecenal, Palmitin, 1,2-di-, 2-aminoethyl hydrogen phosphate and Decyl hexanoate with peak areas of 34.40%, 20.48%, 15.46% and 12.48% respectively. In conclusion, Aloe vera contained many broad spectrum antimicrobially active compounds that can be further purified to produce new antimicrobial compounds that can be used in the treatment of many diseases especially those caused by S. typhi, S. aureus and E. coli.

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“…A condition for GC analyses is that the analytes must be volatile enough to be able to be separated and detected. For this reason, many of the GC applications have been based on the characterization of the volatile compounds fractions of different plant or food matrices [ 23 , 24 , 25 ]. However, several non-volatile compounds can be derivatized prior to analysis in order to increase its volatility.…”

Section: Phytochemical Characterization Of Bioactive Compoundsmentioning

confidence: 99%

The Role of High-Resolution Analytical Techniques in the Development of Functional Foods

Fernández-Ochoa

Leyva-Jiménez²,

Cádiz-Gurrea

et al. 2021

IJMS

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The approaches based on high-resolution analytical techniques, such as nuclear magnetic resonance or mass spectrometry coupled to chromatographic techniques, have a determining role in several of the stages necessary for the development of functional foods. The analyses of botanical extracts rich in bioactive compounds is one of the fundamental steps in order to identify and quantify their phytochemical composition. However, the compounds characterized in the extracts are not always responsible for the bioactive properties because they generally undergo metabolic reactions before reaching the therapeutic targets. For this reason, analytical techniques are also applied to analyze biological samples to know the bioavailability, pharmacokinetics and/or metabolism of the compounds ingested by animal or human models in nutritional intervention studies. In addition, these studies have also been applied to determine changes of endogenous metabolites caused by prolonged intake of compounds with bioactive potential. This review aims to describe the main types and modes of application of high-resolution analytical techniques in all these steps for functional food development.

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GC–MS analysis of volatile organic compounds from Bambara groundnut rhizobacteria and their antibacterial properties

Abstract: Graphical abstract

Cited by 47 publications

References 43 publications

Metabolome profile of osmotolerant rhizobacteria under osmotic stress

Metabolome profile of osmotolerant rhizobacteria under osmotic stress

Phytochemical Screening and Antimicrobial Activity of Aqueous Stem Extract of Aloe vera on Some Common Pathogenic Bacteria

The Role of High-Resolution Analytical Techniques in the Development of Functional Foods

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