GC/MS Fingerprinting of Solvent Extracts and Essential Oils Obtained from Liverwort Species

Ludwiczuk, Agnieszka; Asakawa, Yoshinori

doi:10.1177/1934578x1701200838

Cited by 3 publications

(4 citation statements)

References 48 publications

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“…Quite similar, Ludwiczuk et al [ 21 ], demonstrated the use of volatile compounds as chemical markers for the identification of Conocephalum conicum cryptic species. The importance of the composition of volatile compounds as chemical markers is confirmed by the results of numerous studies of different liverwort species [ 8 , 18 , 19 , 20 , 21 , 22 , 23 ].…”

Section: Resultsmentioning

confidence: 83%

“…Many of these compounds occur only in liverworts and exhibit interesting biological activities [ 9 , 10 , 11 , 12 , 13 , 14 ]. These compounds are also a valuable source of markers for resolving taxonomic problems and identifying individual species [ 15 , 16 , 17 , 18 , 19 , 20 ]. Revealing differences in the composition of chemical compounds between closely related species is important not only for the identification of species but also for the clarification of evolutionary relationships between them [ 19 , 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

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Chemical Fingerprinting of Cryptic Species and Genetic Lineages of Aneura pinguis (L.) Dumort. (Marchantiophyta, Metzgeriidae)

et al. 2021

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Aneura pinguis (L.) Dumort. is a representative of the simple thalloid liverworts, one of the three main types of liverwort gametophytes. According to classical taxonomy, A. pinguis represents one morphologically variable species; however, genetic data reveal that this species is a complex consisting of 10 cryptic species (named by letters from A to J), of which four are further subdivided into two or three evolutionary lineages. The objective of this work was to develop an efficient method for the characterisation of plant material using marker compounds. The volatile chemical constituents of cryptic species within the liverwort A. pinguis were analysed by GC-MS. The compounds were isolated from plant material using the HS-SPME technique. Of the 66 compounds examined, 40 were identified. Of these 40 compounds, nine were selected for use as marker compounds of individual cryptic species of A. pinguis. A guide was then developed that clarified how these markers could be used for the rapid identification of the genetic lineages of A. pinguis. Multivariate statistical analyses (principal component and cluster analysis) revealed that the chemical compounds in A. pinguis made it possible to distinguish individual cryptic species (including genetic lineages), with the exception of cryptic species G and H. The classification of samples based on the volatile compounds by cluster analysis reflected phylogenetic relationships between cryptic species and genetic lineages of A. pinguis revealed based on molecular data.

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Section: Resultsmentioning

confidence: 83%

Section: Introductionmentioning

confidence: 99%

Chemical Fingerprinting of Cryptic Species and Genetic Lineages of Aneura pinguis (L.) Dumort. (Marchantiophyta, Metzgeriidae)

et al. 2021

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“…Liverworts are the only group of plants with characteristic cellular structures, oil bodies, that are organelles surrounded by a single membrane [6,7]. In these structures, chemical compounds are synthesised and stored [8,9]. Chemotaxonomic studies of liverworts have shown the presence of specific compounds at the family, genus, and even species level [9][10][11].…”

Section: Introductionmentioning

confidence: 99%

“…However, in recent times, species identification has often been performed based on molecular tools, including the fast-growing DNA barcoding proposed by [28]. Another method to identify taxonomically difficult species of liverworts is an analysis of the chemical composition of the compounds contained in plants [8,12,13]. Chemical composition analysis is a method for classifying and identifying plants based on confirmed differences and similarities in their biochemical composition.…”

Section: Introductionmentioning

confidence: 99%

Seasonal Variability of Volatile Components in Calypogeia integristipula

Wawrzyniak,

Guzowska,

Wasiak

et al. 2023

Molecules

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Liverworts contain a large number of biologically active compounds that are synthesised and stored in their oil bodies. However, knowledge about the chemical composition of individual species is still incomplete. The subject of the study was Calypogeia integristipula, a species representing leafy liverworts. Plant material for chemotaxonomic studies was collected from various locations in Poland. The chemical composition was determined in 74 samples collected from the natural environment in 2021 and 2022 in three growing seasons: spring, summer and autumn, and for comparison with samples originating from in vitro culture. The plants were classified as Calypogeia integristipula on the basis of morphological characteristics, oil bodies, and DNA markers. The volatile organic compounds (VOCs) from the biological material were extracted by headspace solid phase microextraction (HS-SPME). The samples were then analysed by gas chromatography–mass spectrometry (GC-MS). A total of 79 compounds were detected, of which 44 compounds were identified. The remaining compounds were described using the MS fragmentation spectrum. Cyclical changes in the composition of compounds associated with the growing season of Calypogeia integristipula were observed. Moreover, samples from in vitro culture and samples taken from the natural environment were shown to differ in the composition of chemical compounds. In terms of quantity, among the volatile compounds, compounds belonging to the sesquiterpene group (46.54–71.19%) and sesqiuterpenoid (8.12–22.11%) dominate. A smaller number of compounds belong to aromatic compounds (2.30–10.96%), monoterpenes (0.01–0.07%), monoterpenoids (0.02–0.33%), and aliphatic hydrocarbons (1.11–6.12%). The dominant compounds in the analysed liverworts were: anastreptene (15.27–31.14%); bicyclogermacrene (6.99–18.09%), 4,5,9,10-dehydro-isolongifolene (2.00–8.72%), palustrol (4.95–9.94%), spathulenol (0.44–5.11%).

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Chemical analysis and biological activity of the essential oils and extracts of two liverwort species growing in Turkey

Celik,

Şahin,

Baltaş

et al. 2023

Bot Serb

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The aim of this study was to investigate the chemical composition of the essential oils (EOs) from two Diplophyllum species and to evaluate their bioactivity potential [antimicrobial, antioxidant, anti-urease, anti-xanthine oxidase (XO)], and phenolic compounds. The analysis of Diplophyllum albicans and Diplophyllum taxifolium permitted the identification of 62 components, comprising ? 99.6% of the total EO composition. The major components found in these liverwort species were ?-patchoulene, ?-santalene, ?-himachalene, and cubebol. The antimicrobial assays showed that the solvent extracts (n-hexane and methanol) from these liverwort species exhibited weak to moderate antimicrobial activity. In addition, the methanol extracts of these liverwort species also exhibited moderate to high antioxidant potential. The enzyme inhibitory effects of the species were determined using urease and XO for the methanol extracts. Generally, the methanol extracts of D. albicans and D. taxifolium exhibited powerful urease inhibition with IC50 values of 9.711 ?} 0.058 and 6.304 ?} 0.099 ?g/mL, respectively, compared to the IC50 value of 26.124 ?} 0.077 ?g/mL for the standard (acetohydroxamic acid). Moreover, the HPLC-UV detection method showed that the analysed methanol extracts of these liverwort species contained only catechin and benzoic acid. These findings suggest that the analysed liverwort species possess antioxidant and urease inhibition, thus indicating the potential to explore new bioactive molecules.

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GC/MS Fingerprinting of Solvent Extracts and Essential Oils Obtained from Liverwort Species

Cited by 3 publications

References 48 publications

Chemical Fingerprinting of Cryptic Species and Genetic Lineages of Aneura pinguis (L.) Dumort. (Marchantiophyta, Metzgeriidae)

Chemical Fingerprinting of Cryptic Species and Genetic Lineages of Aneura pinguis (L.) Dumort. (Marchantiophyta, Metzgeriidae)

Seasonal Variability of Volatile Components in Calypogeia integristipula

Chemical analysis and biological activity of the essential oils and extracts of two liverwort species growing in Turkey

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