GC-MS, HPLC, TLC of Dorycnium herbaceum Tincture Species and Synergistic / Antagonist Effect Testing in Combination with Antibiotics

Abstract

The study objectives were to determine the chemical composition and the synergistic / antagonistic effect of the association between hydroalcoholic extract from the Dorycnii pentaphylli herba (DPH) and the antibiotics of choice, on five reference strains. The tincture contains flavonoids and polyphenol carboxylic acids in low concentrations. DPH has an antagonistic effect on three of the drugs tested (amoxicillin + clavulanic acid, levofloxacin, amikacin), the therapeutic effect being completely canceled and has no significant effect on two of them (ceftazidime, cefotaxime).

Method of Determination of Rivanol by Laser Induced Fluoroscence

“…A topical antiseptic that would not only avoid additional compromise of the host's defence reactions but also would positively support the immune system in its efforts to control wound infection will be of substantial clinical importance. Some recent studies indicate that plant extracts can have a synergism effect with different antibiotics and can slow dawn bacterial infection [2].…”

TLC, GC-MS, HPLC Analyses and Testing the Antibacterial Effect of Tragopogon pratensis and Vaccinium myrtillus

“…Equipment and working conditions for HPLC analysis were as follows: HPLC Jasco MD-2015, two-pump, thermostat, UV-DAD detection system, degassing system; eluent A (acetonitrile); eluent B (0.1% phosphoric acid); working gradient: pre-run →10% A, 90% B; 13.1 min. [16].…”

“…Results were expressed as average values obtained by calculating the arithmetic mean of diameters for the three tests. Very small colonies were not considered, neither subsequent invasion of the inhibition zone or discrete increments within the zone of inhibition [16][17][18].…”

Determination of Antioxidant Components and Activity of Tamarix ramosissima Comparative with Vaccinium myrtillus on Streptozotocin-diabetic Mice

“…Analysis by HPLC chromatography technique of flavonoids and polyphenol carboxylic acids of tinctures Equipment and working conditions for HPLC analysis were as follows: HPLC Jasco MD-2015, two-pump, thermostat, UV-DAD detection system, degassing system; eluent A (acetonitrile); eluent B (0.1% phosphoric acid); working gradient: prerun →10% A, 90% B; 13 [12].…”