Gc-MS Profile of in Vivo and in Vitro Shoots of Cleome Gynandra L.

Abstract

Objective: Investigation of the bioactive compounds from the ethanol shoot extracts of in vivo and in vitro plants of Cleome gynandra (C. gynandra) through GC-MS analysis. Methods:The nodal explants were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of 6-benzylaminopurine (BAP), kinetin-6-furfurylaminopurine (Kin) and indole 3 acetic acids (IAA) for shoot induction. In the present study, the phytochemical constituents were analyzed from the ethanol extract of in vivo and in vitro plants of C. gynandra using Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The mass spectrum of the ethanol extract was compared with the available library sources.Results: In the present study, different concentrations of cytokinins and in the combination of IAA are used to develop regenerated shoots. The maximum number of shoots was obtained 9.2±0.41 with the length of 6.6 cm and highest frequency of (100%) shoot induction was observed on MS medium containing 10 μM BAP with 4 μM IAA. The GC-MS analysis revealed that the shoots of in vivo and in vitro plants contained 21phytochemicals, of these 3 components were similar in both in vivo and in vitro plants, 2 phytochemical's are repeated with different RT, 7 components are having biological activity and in the remaining 9 components, biological activities are not reported. Conclusion:The present study, the in vitro regeneration, combinations of hormones (10 μM BAP plus 4 μM IAA) tested showed the best result than individual and also revealed that the synthesis of more number of phytochemicals present in the ethanolic extracts of in vitro plants than the in vivo plants of C. gynandra.