GC‐MS quantitation of isocitric acid in the presence of a large excess of citric acid

Tisza, Sándor; Molnár-Perl, I.

doi:10.1002/jhrc.1240170308

Cited by 15 publications

(6 citation statements)

References 15 publications

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“…Here, we try to evaluate all those parameters which proved to have considerable influence in the quantitation of our target compounds on the basis of their TIC and SFI values. This work is the continuation of recently published papers [2,3,[48][49][50][51] reporting the simultaneous determination of acids and/or sugars as their silyl ester/ether/oxime derivatives, based on their TIC [2,3,49,50], and on their SFI values [2,51].…”

Section: Introductionmentioning

confidence: 97%

“…However, mass detectors serve simultaneously for qualitative and quantitative analysis in most laboratories, taking advantage of the information of the Mass Spectral Database [1]. More attention should be paid on the quantitative data evaluation possibilities offered by the GC-MS. Several efforts have been made that , involve fragmentation in qualitative terms, and this has been applied mostly to aliphatic carboxylic acids with few exceptions [2,3,24,27,31,[45][46][47]51]. Acids have been investigated, both in their free form [4][5][6][7][8][9][10] and as their methyl [11][12][13][14], pentafluorobenzoyl [14,15], TMS [16][17][18][19][20][21][22][23], and tert.-butyldimethylsilyl (TBDMS) [24][25][26][27] esters.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous GC-MS quantitation of phosphoric, aliphatic and aromatic carboxylic acids, proline and hydroxymethylfurfurol as their trimethylsilyl derivatives: In model solutions II

1998

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SummaryFragmentation patterns and quantitation possibilities of gas chromatography-mass spectrometry (GC-MS) with Ion Trap Detection (ITD) are reported for the trimethylsilyl (TMS) derivatives of selected aliphatic and aromatic/cyclohexanoic, mono-, di-and polyhydroxy/ methoxy carboxylic acids, o-phosphoric acid, proline and 5-hydroxymethylfurfurol (HMF) -(common in natural matrices, such as fruits, honey etc.). In order to maintain stability of derivatives, their stock solutions were diluted with hexamethyldisilazane. Quantitation was carried out simultaneously on the basis both of the total ion current (TIC) and selective fragment ion (SFI) values. Data obtained proved that (i) the fragmentation of different TMS acids provided very informative, utilizable characteristics, that were also suitable for quantitation; (

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Simultaneous GC-MS quantitation of phosphoric, aliphatic and aromatic carboxylic acids, proline and hydroxymethylfurfurol as their trimethylsilyl derivatives: In model solutions II

1998

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“…So long as the fragmentation of citric acid produces a major fragment at m/z = 273 (Figure 2, third display line, at 2372-2377 scans) and a very tiny amount of fragment at m/z = 375, isocitric acid produces it (m/z = 375) in a considerable larger quantity (Figure 2, forth display line, at 2403-2407 scans). This difference proved to be appropriate for the distinction and quantitation of isocitric acid in the presence of the enormous excess of citric acid present in citrus fruits [7]. In the case of honeys, citric and isocitric acids are present in commensurable concentrations.…”

Section: Figurementioning

confidence: 97%

“…Selected parts of the chromatograms (2330-2430 scans) obtained from TOT and SFI values [7,14] characteristic for shikimic (SFI = m/z = 204), citric (SFI = m/z = 273), isocitric (SFI = m/z = 375) and for the total of citric/isocitric acids (SFIs = m/z = 273 + 347 + 375). Samples: H2, Linden, G2, Pine and G4, Clover (see also Table III).…”

Section: Indications As Inmentioning

confidence: 99%

Simultaneous GC-MS quantitation of o-phosphoric, aliphatic and aromatic carboxylic acids, proline, hydroxymethylfurfurol and sugars as their TMS derivatives: In honeys

Horváth

Molnár-Perl

1998

Chromatographia

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SummaryA GC-MS procedure is described for the simultaneous quantitatation of the minor and major constituents of honeys, as their trimethylsilyl derivatives, from one solution, by one injection. Selected minor components (aliphatic and aromatic carboxylic acids, members of various homologous series, together with o-phosphoric acid, proline and hydroxymethylfurfurol), have been determined on the basis of their characteristic fragment ions, in the presence of extremely high excess of honeysaccharides. Selective fragmentation of these minor compounds in the ion trap detector provided possibilities for distinguishing them. The method permitted the simultaneous quantitation of o-phosphoric, malic, shikimic, citric/isocitric, quinic, margaric, oleic and stearic acids, hydroxymethylfurfurol and proline with the extremely high sugar contents of honeys (fructose, glucose, galacturonic acid, inositol, sucrose, trehalose, turanose, maltose, gentiobiose, isomaltose, raffinose, erlose, melezitose, maltotriose, panose, isomaltotriose) and allowed the fast evaluation of sugar and acid constituents of fifteen honeys from various floral and geological origin. Results revealed that (i) the minor components varied in the concentration range of 0.0001 to 0.43 %, and, (ii) together with the saccharides of honeys made up the total of identified and determined constituents from 87.8 % to 98.5 %. Quantitative evaluation of Presented at: Balaton Symposium on High-Performance Separation Methods, Si6fok, Hungary, September 3-5, 1997 the minor constituents was performed on the basis of their selective fragment ion values with an average reproducibility of 6.7 % (RSD).

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“…Based on these papers, it became clear that only selected members of acids have been investigated; neither their fragmentation patterns nor the aspects of their GC-MS quantitation have been investigated systematically. The relevancy of this topic is clear, as attested by the facts that: (i) these acids are trace constituents of various natural matrices, usually existing in the presence of an enormous excess of other compounds that are largely aliphatic carboxylic acids, members of various homologous series and sugars of different degree of polymerisation [13][14][15][16][17][18]. (ii) Numerous silyl derivatives of these acids could not be completely resolved in attempting to achieve fast analyses without any preliminary isolations.…”

Section: Introductionmentioning

confidence: 98%

GC-MS quantitation of benzoic and aralkyl carboxylic acids as their trimethylsilyl derivatives: In model solution I

1998

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SummaryThis paper describes the fragmentation patterns and the GC-MS quantitation possibilities of the trimethylsilyl derivatives of thirty-one aromatic carboxylic acids, using ion trap detection (ITD). Sixteen aralkyl carboxylic acids, including those containing a saturated aliphatic side chain {phenylacetic, 2-phenylbutyric, phenylglycolic (mandelic acid), l]-phenyllactic, 3-hydroxyphenylacetic, 13-phenylpyruvic and 3-(4-hydroxyphenyl)-propionic acids} and those with an unsaturated aliphatic side chain {cinnamic, 2-hydroxyeinnamic (o-coumaric), 4-methoxycinnamic, 3-hydroxycinnamic (m-coumaric), 4-hydroxycinnamic (p-coumaric), 4-hydroxy-3-methoxycinnamic (ferulic acid), 3,4-dihydroxycinnamic (caffeic), and 4-dihydroxy-3,5-dimethoxycinnamic (sinapic) acids}, as well as, the fifteen hydroxy(methoxy)benzoic acids {benzoic, 2-hydroxybenzoic (salicylic), 3-hydroxybenzoic, 4-hydroxybenzoic, 3,5-dimethoxybenzoic, 3,4-dimethoxybenzoic (veratric), 2,6-dihydroxybenzoic (y-resorcylic), 3-methoxy-4-hydroxybenzoic (vanillic), 2,5-dihydroxybenzoic (gentisic), 2,4-dihydroxybenzoic (~-resorcylic), 3,4-dihydroxybenzoic (protocatechuic), 3,5-dihydroxybenzoic (a-resorcylic), 2,4,5-trimethoxybenzoic (asaronic), 3,5-dimethoxy-4-hydroxybenzoic (syringic) and 3,4,5,-trihydroxybenzoic (gallic) acids}, provided distinct fragmentation characteristics that were very useful for their identification and simultaneously quantitation. Based on 1-20 ng amounts of acids, very informative ions of high mass with considerable intensities (

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GC‐MS quantitation of isocitric acid in the presence of a large excess of citric acid

Abstract: Summary

Cited by 15 publications

References 15 publications

Simultaneous GC-MS quantitation of phosphoric, aliphatic and aromatic carboxylic acids, proline and hydroxymethylfurfurol as their trimethylsilyl derivatives: In model solutions II

Simultaneous GC-MS quantitation of phosphoric, aliphatic and aromatic carboxylic acids, proline and hydroxymethylfurfurol as their trimethylsilyl derivatives: In model solutions II

Simultaneous GC-MS quantitation of o-phosphoric, aliphatic and aromatic carboxylic acids, proline, hydroxymethylfurfurol and sugars as their TMS derivatives: In honeys

GC-MS quantitation of benzoic and aralkyl carboxylic acids as their trimethylsilyl derivatives: In model solution I

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