Gd(III)-induced Supramolecular Hydrogelation with Enhanced Magnetic Resonance Performance for Enzyme Detection

Hua, Yongquan; Pu, Guojuan; Ou, Caiwen; Zhang, Xiaoli; Wang, Ling; Sun, Jiangtao; Yang, Zhimou; Chen, Minsheng

doi:10.1038/srep40172

Cited by 18 publications

(16 citation statements)

References 59 publications

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“…Similar to many self-assembly peptide molecules, it is well known that Nap-Gly-Phe-Phe (Nap-GFF) peptide sequence could drive the force of self-assembly by π−π interaction because of the benzene rings in the structure of phenylalanine and aromatics group on Nap. 37 However, as glycine and phenylalanine are hydrophobic amino acids, the solubility of peptide with the sequence of Nap-GFF is poor in water. 26 In this work, we utilized the self-assembly capability of Nap-GFF sequence and further improved the solubility by adding lysine (K) with an amino group and histidine (H) with imidazole to the Nap-GFF sequence.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Similar to many self-assembly peptide molecules, it is well known that Nap-Gly-Phe-Phe (Nap-GFF) peptide sequence could drive the force of self-assembly by π–π interaction because of the benzene rings in the structure of phenylalanine and aromatics group on Nap . However, as glycine and phenylalanine are hydrophobic amino acids, the solubility of peptide with the sequence of Nap-GFF is poor in water .…”

Section: Resultsmentioning

confidence: 99%

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Instant Self-Assembly Peptide Hydrogel Encapsulation with Fibrous Alginate by Microfluidics for Infected Wound Healing

Cui

et al. 2020

ACS Biomater. Sci. Eng.

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Infected wounds caused by persistent inflammation exhibit poor vascularization and cellular infiltration. In order to rapidly control the inflammatory effect and accelerate wound healing, it is necessary to develop a novel drug vehicle addressing the need for infected wounds. Herein, we developed a novel dual-drug delivery system with micrometer-scale alginate fibers encapsulated in instant self-assembly peptide hydrogel. Short peptides with the sequence of Nap-Gly-Phe-Phe-Lys-His (Nap-GFFKH) could self-assemble outside the microfluidic-based alginate microfibers in weak acidic solution (pH ≈ 6.0) within 5 s. The gelation condition is close to the pH environment of the human skin. We further constructed recombinant bovine basic fibroblast growth factor (FGF-2) in fibrous alginate, which was encapsulated in antibiotic-loaded peptide hydrogel. The dual-drug delivery system exhibited good mechanical property and sustained release profiles, where antibiotic could be rapidly released from the peptide hydrogel, while the growth factor could be gradually released within 7 days. Both in vitro antibacterial experiments and in vivo animal experiments confirmed that such a dual-drug delivery system has good antibacterial activity and enhances wound healing property. We suggested that the dual-drug delivery system could be potentially applied for controlled drug release in infected wound healing, drug combination for melanoma therapy, and tissue engineering.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Instant Self-Assembly Peptide Hydrogel Encapsulation with Fibrous Alginate by Microfluidics for Infected Wound Healing

Cui

et al. 2020

ACS Biomater. Sci. Eng.

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“…Self-assembling peptides containing D-amino acids are more stable because natural proteases can readily degrade L-form peptide bonds, but cannot degrade D-form peptide bonds. [26][27][28][29] A recent study revealed that self-assembling peptide hydrogels with β-sheet structures, made of D-amino acids, exhibit high resistance against enzymatic hydrolysis. 30 The conjugation of bioactivity-mimicking peptides to the backbone of self-assembling β-sheets derived from D-amino acids may result in improved biostability and bioactivity for potential therapeutic applications.…”

Section: Introductionmentioning

confidence: 99%

<p>Delivery of MSCs with a Hybrid β-Sheet Peptide Hydrogel Consisting IGF-1C Domain and D-Form Peptide for Acute Kidney Injury Therapy</p>

Wang¹,

Shang²,

Chen³

et al. 2020

IJN

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By providing a stem cell microenvironment with particular bioactive constituents in vivo, synthetic biomaterials have been progressively successful in stem cell-based tissue regeneration by enhancing the engraftment and survival of transplanted cells. Designs with bioactive motifs to influence cell behavior and with D-form amino acids to modulate scaffold stability may be critical for the development and optimization of self-assembling biomimetic hydrogel scaffolds for stem cell therapy. Materials and Methods: In this study, we linked naphthalene (Nap) covalently to a short D-form peptide (Nap-D F D FG) and the C domain of insulin-like growth factor-1 (IGF-1C) as a functional hydrogel-based scaffolds, and we hypothesized that this hydrogel could enhance the therapeutic efficiency of human placenta-derived mesenchymal stem cells (hP-MSCs) in a murine acute kidney injury (AKI) model. Results: The self-assembling peptide was constrained into a classical β-sheet structure and showed hydrogel properties. Our results revealed that this hydrogel exhibited increased affinity for IGF-1 receptor. Furthermore, cotransplantation of the β-IGF-1C hydrogel and hP-MSCs contributed to endogenous regeneration post-injury and boosted angiogenesis in a murine AKI model, leading to recovery of renal function. Conclusion: This hydrogel could provide a favorable niche for hP-MSCs and thereby rescue renal function in an AKI model by promoting cell survival and angiogenesis. In conclusion, by covalently linking the desired functional groups to D-form peptides to create functional hydrogels, self-assembling β-sheet peptide hydrogels may serve as a promising platform for tissue-engineering and stem cell therapy.

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“…[131,133–135] The subsequent hydrogel formation requires retention of these aligned structures and their assembly which is usually achieved by the addition of ions or pH change. [131,136] The switchable nature of the magnetic field coupled with the slow diffusion of ions enables precise control of the anisotropy across the networks, something that is generally very difficult to achieve.…”

Section: Physical Stimuli-responsive Hydrogelsmentioning

confidence: 99%

Stimuli‐Responsive Supramolecular Hydrogels and Their Applications in Regenerative Medicine

Hoque

Sangaj

Varghese

2018

Macromolecular Bioscience

147

108

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Supramolecular hydrogels are a class of self-assembled network structures formed via non-covalent interactions of the hydrogelators. These hydrogels capable of responding to external stimuli are considered to be smart materials due to their ability to undergo sol–gel and/or gel–sol transition upon subtle changes in their surroundings. Such stimuli-responsive hydrogels are intriguing biomaterials with applications in tissue engineering, delivery of cells and drugs, modulating tissue environment to promote innate tissue repair, and imaging for medical diagnostics among others. This review summarizes the recent developments in stimuli-responsive supramolecular hydrogels and their potential applications in regenerative medicine. Specifically, various structural aspects of supramolecular hydrogelators involved in self-assembly, the role of external stimuli in tuning/controlling their phase transitions, and how these functions could be harnessed to advance applications in regenerative medicine are focused on. Finally, the key challenges and future prospects for these versatile materials are briefly described.

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Gd(III)-induced Supramolecular Hydrogelation with Enhanced Magnetic Resonance Performance for Enzyme Detection

Cited by 18 publications

References 59 publications

Instant Self-Assembly Peptide Hydrogel Encapsulation with Fibrous Alginate by Microfluidics for Infected Wound Healing

Instant Self-Assembly Peptide Hydrogel Encapsulation with Fibrous Alginate by Microfluidics for Infected Wound Healing

<p>Delivery of MSCs with a Hybrid β-Sheet Peptide Hydrogel Consisting IGF-1C Domain and D-Form Peptide for Acute Kidney Injury Therapy</p>

Stimuli‐Responsive Supramolecular Hydrogels and Their Applications in Regenerative Medicine

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