GDSL lipase‐like 1 regulates systemic resistance associated with ethylene signaling in Arabidopsis

Abstract

SummarySystemic resistance is induced by necrotizing pathogenic microbes and non-pathogenic rhizobacteria and confers protection against a broad range of pathogens. Here we show that Arabidopsis GDSL LIPASE-LIKE 1 (GLIP1) plays an important role in plant immunity, eliciting both local and systemic resistance in plants. GLIP1 functions independently of salicylic acid but requires ethylene signaling. Enhancement of GLIP1 expression in plants increases resistance to pathogens including Alternaria brassicicola, Erwinia carotovora and Pseudomonas syringae, and limits their growth at the infection site. Furthermore, local treatment with GLIP1 proteins is sufficient for the activation of systemic resistance, inducing both resistance gene expression and pathogen resistance in systemic leaves. The PDF1.2-inducing activity accumulates in petiole exudates in a GLIP1-dependent manner and is fractionated in the size range of less than 10 kDa as determined by size exclusion chromatography. Our results demonstrate that GLIP1-elicited systemic resistance is dependent on ethylene signaling and provide evidence that GLIP1 may mediate the production of a systemic signaling molecule(s).

The Lectin Receptor Kinase-VI.2 Is Required for Priming and Positively Regulates Arabidopsis Pattern-Triggered Immunity

“…Pst bacteria were cultivated at 288C and 340 rpm in King's B medium containing rifampicin (Pst DC3000), rifampicin, spectinomycin, and kanamycin (DB29) or rifampicin and kanamycin (CB200). We obtained Pcc hp-and hrc-deficient strain WPP17 (Yap et al, 2004) from Amy Charkowski (University of Wisconsin, Madison, WI), and Pcc SCC1 (Kariola et al, 2005) expressing the green fluorescent protein (Kwon et al, 2009) were a gift from Ohkmae Park (Korea University, Seoul, Korea). Pcc bacteria were cultivated at 288C and 340 rpm in Luria-Bertani medium (Bioman Scientific) for 16 to 18 h without selection (Pcc WPP17) or with ampicillin (Pcc SCC1).…”

Necrotroph Attacks on Plants: Wanton Destruction or Covert Extortion?

“…GLIP1 encodes a secreted antimicrobial protein proposed to function in the generation and amplifi cation of signals required for ET-mediated systemic resistance (Oh et al, 2005;Kwon et al, 2009). Plant treatment with recombinant GLIP1 or its constitutive expression in planta confers local and systemic protection against A. brassicicola and E. carotovora (Kwon et al, 2009). GLIP1-elicited resistance correlates with increased systemic expression of PDF1.2 and is dependent on ET-signaling mediated by ETR1.…”

“…Thus, ET may modulate defense through its effects on cell death or interaction with other hormone responses. ETR1 is also involved in the regulation of resistance mediated by Arabidopsis GDSL LIPASE-LIKE 1 (GLIP1) (Kwon et al, 2009). GLIP1 encodes a secreted antimicrobial protein proposed to function in the generation and amplifi cation of signals required for ET-mediated systemic resistance (Oh et al, 2005;Kwon et al, 2009).…”

“…ETR1 is also involved in the regulation of resistance mediated by Arabidopsis GDSL LIPASE-LIKE 1 (GLIP1) (Kwon et al, 2009). GLIP1 encodes a secreted antimicrobial protein proposed to function in the generation and amplifi cation of signals required for ET-mediated systemic resistance (Oh et al, 2005;Kwon et al, 2009). Plant treatment with recombinant GLIP1 or its constitutive expression in planta confers local and systemic protection against A. brassicicola and E. carotovora (Kwon et al, 2009).…”

Identification of low potassium stress-responsive proteins in tobacco (Nicotiana tabacum) seedling roots using an iTRAQ-based analysis

“…In our study, the expression levels of two lipases increased. The overexpression of the lipase gene CaGLIP1 in Arabidopsis increases drought tolerance (Hong et al, 2008), and lipases from Arabidopsis are involved in ethylene and auxin signaling (Kwon et al, 2009;Lee et al, 2009). Therefore, lipases have a close relationship with low potassium conditions.…”