Gel Chromatography

Determann, Helmut

doi:10.1007/978-3-642-49886-2

Cited by 80 publications

(24 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Arich RNA sequences were recovered either by precipitation with 2 vol. ethanol in the presence of 3O/, NaCl overnight at -20 "C or by desalting by centrifugation twice through 10-ml columns of Sephadex G-25 (fine) in water followed by freeze-drying [24].…”

Section: "C [20]mentioning

confidence: 99%

See 1 more Smart Citation

The Poly(adenylic acid) Sequence of Mouse Globin Messenger RNA

Mansbridge

Crossley

Lanyon

et al. 1974

European Journal of Biochemistry

View full text Add to dashboard Cite

Mouse globin messenger RNA has been degraded by ribonuclease T, to give poly(adeny1ic acid) fragments of two size classes which run electrophoretically with standard RNA molecules approximately 75 and 50 nucleotides long. These contain no internal ribonucleotides other than adenosine and occur at the 3'-end of the mRNA molecule. The probable sequence to the 5'-side of the poly(A) tract is -G-C. Attempts to separate the DL and B mRNA activities by formamide gradient elution from poly(U)-Sepharose were unsuccessful.The evidence is more compatible with a post-transcriptional end-addition mechanism for t,he synthesis of the poly(adeny1ic acid) region of messenger RNA than a transcriptional mechanism.

show abstract

Section: "C [20]mentioning

confidence: 99%

“…Unreacted [32P]ATP was removed by centrifugation of the reaction mixture through a 1-ml column of Sephadex G-25 (fine) in buffer I [24]. The labelled fragments were then isolated on poly(U)-Sepharose as described above.…”

Section: "C [20]mentioning

confidence: 99%

The Poly(adenylic acid) Sequence of Mouse Globin Messenger RNA

Mansbridge

Crossley

Lanyon

et al. 1974

European Journal of Biochemistry

View full text Add to dashboard Cite

show abstract

“…Note that the region of high GA concentrations is proximal to the conditions in which the solubility of drugs was measured, this being additional evidence for the suggested mechanism of water solubility increase. An explanation for the decreasing MM differences on dilution may be that drug molecules can escape from GA micelles during GPC process to diluted solutions and appear in chromatograms as individual substances eluted at different rates [45]. Anyway, further investigation is needed to gain more insights into the GA-drug interaction.…”

Section: Gpc Of Dissolved Ga-drug Solid Dispersionsmentioning

confidence: 99%

Complexes of Polysaccharides and Glycyrrhizic Acid with Drug Molecules − Mechanochemical Synthesis and Pharmacological Activity

Душкин¹,

Толстикова²,

Хвостов³

et al. 2012

The Complex World of Polysaccharides

View full text Add to dashboard Cite

“…METHODS Cryoprecipitated fractions of human plasma were prepared from blood drawn from the antecubital veins of normal adult volunteers, using polystyrene syringes coated with silicone oil 15 min at 3500 rpm. The supernatant plasma was distributed in 30-ml aliquots into silicone-coated Lusteroid tubes, and quick-frozen by immersion in a mixture of 95% ethanol and dry ice.…”

Section: Introductionmentioning

confidence: 99%

“…J. ), a spherical agarose gel with particle size of 30-200u in the swollen state and approximate fractionation range for molecular weights of 1 X 104 to 3 X 106 (15), was added to sufficient barbital-saline buffer to make a thick slurry. A 33 X 0.9 cm glass column was filled with barbitalsaline buffer and the outlet tubing was raised to the level of the fluid meniscus.…”

Section: Introductionmentioning

confidence: 99%