Gel dressing based on type I collagen modified with oligourethane and silica for skin wound healing

Abstract

Cutaneous wound healing is a complex process that leads the skin reparation with the formation of scar tissue that typically lacks skin appendages. This fact drives us to find new strategies to improve regenerative healing of the skin. This study outlines, the contribution of colloidal silica particles and oligourethane crosslinking on the collagen material properties and the effect on skin wound healing in rats. We characterized the gel properties that are key for in-situ gelation, which is accomplished by the latent reactivity of oligourethane bearing blocked isocyanate groups to crosslink collagen while entrapping silica particles. The swelling/degradation behavior and the elastic modulus of the composite gel were consistent with the modification of collagen type I with oligourethane and silica. On the other hand, these gels were characterized as scaffold for murine macrophages and human stem cells. The application of a composite gel dressing on cutaneous wounds showed a histological appearance of the recovered skin as intact skin; featured by the epidermis, hair follicles, sebaceous glands, subcutaneous adipose layer, and dermis. The results suggest that the collagen-based composite dressings are promising modulators in skin wound healing to achieve a regenerative skin closure with satisfactory functional and aesthetic scars.

Novel polycaprolactone (PCL)‐type I collagen core‐shell electrospun nanofibers for wound healing applications

“…The production of electrospun nanofibers from several biopolymers, among them collagen, has been intensively studied in recent years 26,57,58 . The interaction with certain solvents, especially halogenated, and the shear forces resulting from the electrospinning can lead to the breakage of specific interchain bonding interactions that maintain the cohesion of the triple helix structure of collagen molecules 28,30,36,59 …”

“…26,57,58 The interaction with certain solvents, especially halogenated, and the shear forces resulting from the electrospinning can lead to the breakage of specific interchain bonding interactions that maintain the cohesion of the triple helix structure of collagen molecules. 28,30,36,59 The coaxial electrospinning of Col_1/PVA and PCL was investigated aiming to obtain core-shell nanofibers without beads or pearls and with relatively uniform diameters. The experiments were designed to have a shell formed by a Col_1/PVA phase, while the core was formed by the PCL.…”

“…In general, endogenous native collagen can fulfill its role in normal wound healing. However, in chronic wounds, the use of collagen-based dressing is necessary to improve and accelerate the healing process, 29,30 since exogenous collagen produces a chemotactic stimulus to macrophages, granulocytes, and fibroblasts and can function as a sacrificial substrate to matrix metalloproteinases. Some previous works have reported the collagen electrospinning process.…”

Gas-jet propelled hemostats for targeted hemostasis in wounds with irregular shape and incompressibility

“…Currently, a variety of polymers, such as chitosan, 22 calcium alginate, 23 fibrin, 24 gelatin, 25 collagen, 26 oxidized cellulose, 27 and oxidized regenerated cellulose, 28 are widely used in biomaterials due to their superior high water absorption, fibrinogen adsorption, macrophage phagocytosis activation, platelet aggregation promotion, and antibacterial activity. Given its strong bioactivity in hemostasis, low antigenicity, outstanding biocompatibility, and high cell proliferation, collagen—a naturally occurring, biodegradable, negatively charged macromolecule—has been widely used as a hemostatic dressing, 29 wound dressing, 30 and tissue engineering scaffold. 31,32 Additionally, collagen has the ability to stimulate platelet adhesion and aggregation as well as the release of coagulation factors to aid in blood coagulation.…”

Silk Fibroin Biomaterials and Their Beneficial Role in Skin Wound Healing

“…Several natural materials like collagen [ 7 , 8 , 9 ], gelatine [ 10 , 11 , 12 ], keratin [ 13 , 14 , 15 ], and silk [ 16 , 17 , 18 ] have gained much attention in tissue engineering and regenerative medicine as potential skin wound dressings. Especially noteworthy are wound dressings based on silk fibroin (SF), characterized by good tissue biocompatibility and biodegradability.…”