A solution of DNA (salmon testes, 2.5% w/w) in a bio‐based deep eutectic solvent consisting of the mixture of choline chloride and ethylene glycol (Chol.Cl–EG 1:2) is turned to a soft ion gel upon addition of 6 n HCl (pH 2.9) at room temperature (25 °C). The gel structure is lost when 6 n NaOH (pH 7.3) is added, and the structure could be regained at pH 2.9, and vice versa. The DNA regenerated from the solution at pH 7.3 shows the presence of spheroid‐shaped micro‐structured assemblies under scanning electron (SEM), atomic force (AFM), and transition electron (TEM) microscopes, and forms pH responsive micro‐hydrogel in water.