Gelatin and Gelatin–Polyelectrolyte Complexes: Drug Delivery

Devi, Nirmala; Deka, Chayanika; Maji, Tarun K.; Kakati, Dilip Kumar

doi:10.1081/e-ebpp-120049954

Cited by 12 publications

(8 citation statements)

References 105 publications

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“…This ionization leads to the possible formation of anion-cation interaction. According to the literature, the formation of PEC should be confirmed with FTIR, DSC, and drug release profile [ 4 , 8 , 16 , 56 , 63 , 64 ].…”

Section: Resultsmentioning

confidence: 99%

Mucoadhesive Gelatin Buccal Films with Propranolol Hydrochloride: Evaluation of Mechanical, Mucoadhesive, and Biopharmaceutical Properties

et al. 2021

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This study processes and characterizes propranolol hydrochloride/gelatin mucoadhesive buccal films. Two types of gelatin are used: Gelatin from porcine skin, type A (GA), and gelatin from bovine skin (GB). The influence of gelatin type on mechanical, mucoadhesive, and biopharmaceutical characteristics of buccal films is evaluated. Fourier-Transfer infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) analysis show that GA with propranolol hydrochloride (PRH) in the film (GAP) formed a physical mixture, whereas GB with PRH (GBP) form a compound-complex. Results of mechanical testing (tensile test, hardness) revealed that GAP films exhibit higher elastic modulus, tensile strength, and hardness. A mucoahesion test shows that GBP has higher adhesion strength, while GAP shows higher work of adhesion. Both in vitro release study and in silico simulation indicated that processed films can provide effective drug transport through the buccal mucosa. In silico simulation shows improved bioavailability from buccal films, in comparison to the immediate-release tablets—indicating that the therapeutic drug dose can be markedly reduced.

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Section: Resultsmentioning

confidence: 99%

Mucoadhesive Gelatin Buccal Films with Propranolol Hydrochloride: Evaluation of Mechanical, Mucoadhesive, and Biopharmaceutical Properties

et al. 2021

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“…Biomaterials mimicking features of the extracellular matrix (ECM) have been studied extensively for the past decades as artificial scaffolds for tissue engineering − and for controlled drug delivery. − In the context of soft hydrogel materials, gelatin, a hydrolysis product of collagen, is particularly interesting due to its inherent biocompatibility and biodegradability. − However, the physical hydrogels formed by gelatin in aqueous solution lack mechanical stability at human body temperature, making chemical cross-linking of gelatin hydrogels often indispensable . Chemically cross-linked gelatin-based hydrogels were, for example, obtained by addition of cross-linkers such as glutaraldehyde or carbodiimide (e.g., N -(3-dimethylaminopropyl)- N ′-ethylcarbodiimide hydrochloride), by direct enzymatic cross-linking (e.g., transglutaminase, or laccase and tyrosinase) or by chemical modification of amino acid residues, for example, for enzymatic cross-linking with horseradish peroxidase or for radical cross-linking by introducing methacryl groups …”

Section: Introductionmentioning

confidence: 99%

Quantification of Substitution of Gelatin Methacryloyl: Best Practice and Current Pitfalls

et al. 2017

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Cross-linkable gelatin methacryloyl (GM) is widely used for the generation of artificial extracellular matrix (ECM) in tissue engineering. However, the quantification of modified groups in GM is still an unsolved issue, although this is the key factor for tailoring the physicochemical material properties. In this contribution, H-C-HSQC NMR spectra are used to gain detailed structural information on GMs and of 2-fold modified gelatin containing methacryloyl and acetyl groups (GMAs). Distinctive identification of methacrylate, methacrylamide, and acetyl groups present in GMs and GMAs revealed an overlap of methacrylamide and modified hydroxyproline signals in the H NMR spectrum. Considering this, we suggest a method to quantify methacrylate and methacrylamide groups in GMs precisely based on simpleH NMR spectroscopy with an internal standard. Quantification of acetylation in GMAs is also possible, yet, 2D NMR spectra are necessary. The described methods allow direct quantification of modified groups in gelatin derivatives, making them superior to other, indirect methods known so far.

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“…Gelatin is a common matrix for tissue engineering [12,13] and controlled release systems [13,14,15,16,17,18]. Gelatins are available from different animal sources via different hydrolysis processes [19,20], which influence the physicochemical properties of the hydrogels, and release properties for encapsulated active agents.…”

Section: Introductionmentioning

confidence: 99%

Controlled Release of Vascular Endothelial Growth Factor from Heparin-Functionalized Gelatin Type A and Albumin Hydrogels

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Tovar

et al. 2017

Gels

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Bio-based release systems for pro-angiogenic growth factors are of interest, to overcome insufficient vascularization and bio-integration of implants. In this study, we investigated heparin-functionalized hydrogels based on gelatin type A or albumin as storage and release systems for vascular endothelial growth factor (VEGF). The hydrogels were crosslinked using carbodiimide chemistry in presence of heparin. Heparin-functionalization of the hydrogels was monitored by critical electrolyte concentration (CEC) staining. The hydrogels were characterized in terms of swelling in buffer solution and VEGF-containing solutions, and their loading with and release of VEGF was monitored. The equilibrium degree of swelling (EDS) was lower for albumin-based gels compared to gelatin-based gels. EDS was adjustable with the used carbodiimide concentration for both biopolymers. Furthermore, VEGF-loading and release were dependent on the carbodiimide concentration and loading conditions for both biopolymers. Loading of albumin-based gels was higher compared to gelatin-based gels, and its burst release was lower. Finally, elevated cumulative VEGF release after 21 days was determined for albumin-based hydrogels compared to gelatin A-based hydrogels. We consider the characteristic net charges of the proteins and degradation of albumin during release time as reasons for the observed effects. Both heparin-functionalized biomaterial systems, chemically crosslinked gelatin type A or albumin, had tunable physicochemical properties, and can be considered for controlled delivery of the pro-angiogenic growth factor VEGF.

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Gelatin and Gelatin–Polyelectrolyte Complexes: Drug Delivery

Cited by 12 publications

References 105 publications

Mucoadhesive Gelatin Buccal Films with Propranolol Hydrochloride: Evaluation of Mechanical, Mucoadhesive, and Biopharmaceutical Properties

Mucoadhesive Gelatin Buccal Films with Propranolol Hydrochloride: Evaluation of Mechanical, Mucoadhesive, and Biopharmaceutical Properties

Quantification of Substitution of Gelatin Methacryloyl: Best Practice and Current Pitfalls

Controlled Release of Vascular Endothelial Growth Factor from Heparin-Functionalized Gelatin Type A and Albumin Hydrogels

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