Gelatin-Based 3D Microgels for In Vitro T Lineage Cell Generation

Suraiya, Anisha B.; Hun, Michael L.; Truong, Vinh X.; Forsythe, John S.; Chidgey, Ann Patricia

doi:10.1021/acsbiomaterials.9b01610

Cited by 15 publications

(22 citation statements)

References 56 publications

(120 reference statements)

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“…Recently, microcarrier gelatin‐PEG hydrogels produced using a microfluidic system were tested for the generation of thymic‐like organoids. [ 80 ] In order to create a scalable approach, OP9‐DL4 cells were encapsulated in gelatin‐PEG hydrogels, showing high viability, a well‐spread morphology, and the tendency to migrate towards the surface of the microgel. When co‐cultured with murine liver HSPCs, the differentiation profiles obtained were similar to the ones of 2D cultures, but better than the Matrigel.…”

Section: Thymus Tissue Engineeringmentioning

confidence: 99%

Recapitulation of Thymic Function by Tissue Engineering Strategies

Silva

Reis

Martins

et al. 2021

Adv Healthcare Materials

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The thymus is responsible for the development and selection of T lymphocytes, which in turn also participate in the maturation of thymic epithelial cells. These events occur through the close interactions between hematopoietic stem cells and developing thymocytes with the thymic stromal cells within an intricate 3D network. The complex thymic microenvironment and function, and the current therapies to induce thymic regeneration or to overcome the lack of a functional thymus are herein reviewed. The recapitulation of the thymic function using tissue engineering strategies has been explored as a way to control the body's tolerance to external grafts and to generate ex vivo T cells for transplantation. In this review, the main advances in the thymus tissue engineering field are disclosed, including both scaffold-and cell-based strategies. In light of the current gaps and limitations of the developed systems, the design of novel biomaterials for this purpose with unique features is also discussed.

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Section: Thymus Tissue Engineeringmentioning

confidence: 99%

Recapitulation of Thymic Function by Tissue Engineering Strategies

Silva

Reis

Martins

et al. 2021

Adv Healthcare Materials

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“…This is similar to our previous study in which MSCs, as well as other cell types, migrated to the periphery of microgels. [16][17][18]33 We hypothesize that this may be due to the cells actively sensing higher mass transfer and higher nutrient concentrations at the surface of the microgels, thus migrating to the surface. In addition, this data suggests that the reduced amount of gelatin in the Gel:HA hydrogels was still sufficient to support adequate cell adhesion and spreading similar to that of gelatin alone with a change of total weight percentage from 4 to 2%.…”

Section: Cell Viability and Morphologymentioning

confidence: 99%

Interplay of Hydrogel Composition and Geometry on Human Mesenchymal Stem Cell Osteogenesis

Shrestha

Truong

et al. 2020

Biomacromolecules

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Microgels are emerging as an outstanding platform for tissue regeneration because they overcome issues associated with conventional bulk/macroscopic hydrogels such as limited cell−cell contact and cell communication and low diffusion rates. Owing to the enhanced mass transfer and injectability via a minimally invasive procedure, these microgels are becoming a promising approach for bone regeneration applications. Nevertheless, there still remains a huge gap between the understanding of how the hydrogel matrix composition can influence cell response and overall tissue formation when switching from bulk formats to microgel format, which is often neglected or rarely studied. Here, we fabricated polyethylene glycol-based microgels and bulk hydrogels incorporating gelatin and hyaluronic acid (HA), either individually or together, and assessed the impact of both hydrogel composition and format upon the osteogenic differentiation of encapsulated human bone marrow-derived mesenchymal stem cells (hBMSCs). Osteogenesis was significantly greater in microgels than bulk hydrogels for both gelatin alone (Gel) and gelatin HA composite (Gel:HA) hydrogels, as determined by the expression of Runt-related transcription factor (Runx2) and alkaline phosphatase (ALP) genes and mineral deposition. Interestingly, Gel and Gel:HA hydrogels behaved differently between bulk and microgel format. In bulk format, overall osteogenic outcomes were better in Gel:HA hydrogels, but in microgel format, while the level of osteogenic gene expression was equivalent between both compositions, the degree of mineralization was reduced in Gel:HA microgels. Investigation into the affinity of hydroxyapatite for the different matrix compositions indicated that the decreased mineralization of Gel:HA microgels was likely due to a low affinity of hydroxyapatite to bind to HA and support mineral deposition, which has a greater impact on microgels than bulk hydrogels. Together, these findings suggest that both hydrogel composition and format can determine the success of tissue formation and that there is a complex interplay of these two factors on both cell behavior and matrix deposition. This has important implications for tissue engineering, showing that hydrogel composition and geometry must be evaluated together when optimizing conditions for cell differentiation and tissue formation.

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“…Thus far, a number of polymers have been applied to build drug delivery systems. The natural polymers, including chitosan [ 11 , 12 ], gelatin [ 13 , 14 ], polylactic acid [ 15 , 16 ], polysiloxane [ 17 , 18 ], collagen [ 19 , 20 ], and alginate [ 21 , 22 ] appeared to be good and useful materials. In addition to these compounds, the synthetic polymers were also used.…”

Section: Introductionmentioning

confidence: 99%

Activity of Povidone in Recent Biomedical Applications with Emphasis on Micro- and Nano Drug Delivery Systems

2021

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Due to the unwanted toxic properties of some drugs, new efficient methods of protection of the organisms against that toxicity are required. New materials are synthesized to effectively disseminate the active substance without affecting the healthy cells. Thus far, a number of polymers have been applied to build novel drug delivery systems. One of interesting polymers for this purpose is povidone, pVP. Contrary to other polymeric materials, the synthesis of povidone nanoparticles can take place under various condition, due to good solubility of this polymer in several organic and inorganic solvents. Moreover, povidone is known as nontoxic, non-carcinogenic, and temperature-insensitive substance. Its flexible design and the presence of various functional groups allow connection with the hydrophobic and hydrophilic drugs. It is worth noting, that pVP is regarded as an ecofriendly substance. Despite wide application of pVP in medicine, it was not often selected for the production of drug carriers. This review article is focused on recent reports on the role povidone can play in micro- and nano drug delivery systems. Advantages and possible threats resulting from the use of povidone are indicated. Moreover, popular biomedical aspects are discussed.

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Gelatin-Based 3D Microgels for In Vitro T Lineage Cell Generation

Cited by 15 publications

References 56 publications

Recapitulation of Thymic Function by Tissue Engineering Strategies

Recapitulation of Thymic Function by Tissue Engineering Strategies

Interplay of Hydrogel Composition and Geometry on Human Mesenchymal Stem Cell Osteogenesis

Activity of Povidone in Recent Biomedical Applications with Emphasis on Micro- and Nano Drug Delivery Systems

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