Gelatin/calcium chloride electrospun nanofibers for rapid hemostasis

Yu, Xinrong; Gao, Zichun; Mu, Jiaxiang; Lian, He; Meng, Zhaoxu

doi:10.1039/d2bm01767a

Cited by 32 publications

(15 citation statements)

References 52 publications

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“…Figure a exhibits the clotting time of the different fibrous membranes. Compared to the blank group, the mean clotting time of gelatin was sharply reduced from 775 to 581 s, indicating that gelatin nanofibers could effectively promote coagulation owing to their good adsorption capacity and large specific surface area. − Furthermore, Gel@Ze presented the shortest clotting time (433 s) contributed by the synergistic clotting effect between gelatin nanofibers and zeolite powders. Although the clotting time of the Gel@Ze@PDA (501 s) and Janus membrane (526 s) increased slightly, they showed excellent coagulation properties.…”

Section: Resultsmentioning

confidence: 96%

“…Furthermore, the results of the contact angle tests confirmed the opposite wettability of the two layers. As shown in Figure c, the water droplet was soon spreading on the surface of the Gel@Ze@PDA membrane (hydrophilic layer), and the water contact angle (WCA) decreased from 10 to 0° within 8 s (Movie S2), which enabled blood to spread quickly across the fibrous membrane and the blood cells to attach to the fibers. ,,, On the contrary, the PCL layer presented a relatively high WCA of 131 ± 2°, and it remained nearly constant for even over 3 min (Figure d, Movie S3). The excellent hydrophobic property of the PCL enabled the Janus membrane to maintain its original shape, effectively seal the wound, and control bleeding.…”

Section: Resultsmentioning

confidence: 99%

“…As shown in Figure 3c, the water droplet was soon spreading on the surface of the Gel@Ze@PDA membrane (hydrophilic layer), and the water contact angle (WCA) decreased from 10 to 0°w ithin 8 s (Movie S2), which enabled blood to spread quickly across the fibrous membrane and the blood cells to attach to the fibers. 4,15,40,41 On the contrary, the PCL layer presented a relatively high WCA of 131 ± 2°, and it remained nearly constant for even over 3 min (Figure 3d, Movie S3). The (a) Photographs of E. coli colonies after treatments with PBS, Gel@Ze@PDA fibrous membrane, and Gel@Ze@PDA/PCL fibrous membrane.…”

Section: Wettability Of the Janus Membranementioning

confidence: 98%

See 2 more Smart Citations

One-step Preparation of Antibacterial Gelatin/Polycaprolactone Nanofibrous Janus Membranes for Efficient Hemostasis

Song,

Zhu,

Wang

et al. 2023

ACS Appl. Polym. Mater.

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One cannot overstate the advantages of an ideal hemostasis material for the emergency rescue of wars and traffic accident victims, and it can significantly mitigate patient distress and minimize threat to life. Nonetheless, it is still a daunting task to obtain a hemostasis material encompassing desirable advantages, including safety, antibacterial activity, high efficiency, sufficient mechanical strength, cost-effectiveness, and so on. In this study, a one-step electrospinning strategy for achieving an efficient hemostatic membrane with a Janus structure is provided. Owing to the synergistic coagulation effect between gelatin nanofibers and zeolite powders (hydrophilic layer), the membrane exhibits excellent coagulation performance in vitro. Simultaneously, the hydrophobic layer's efficacy in preventing blood permeation further shortens bleeding time and lessens blood loss in vivo. Moreover, the membrane presents commendable flexibility, antibacterial activity, hemocompatibility, and noncytotoxicity, with promise to be used as a safe and efficient hemostatic dressing in clinical applications.

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Section: Resultsmentioning

confidence: 96%

Section: Resultsmentioning

confidence: 99%

Section: Wettability Of the Janus Membranementioning

confidence: 98%

See 1 more Smart Citation

One-step Preparation of Antibacterial Gelatin/Polycaprolactone Nanofibrous Janus Membranes for Efficient Hemostasis

Song,

Zhu,

Wang

et al. 2023

ACS Appl. Polym. Mater.

View full text Add to dashboard Cite

show abstract

“…Compared with commercially available hemostatic sponges and hemostatic gauze, the gelatin membrane containing calcium ions had a lower hemolysis rate and shorter coagulation time, and a good ex vivo and in vivo hemostatic ability. In addition, it can be adhered to the wound to reduce bleeding and hemostasis time …”

Section: Biological Hemostatic Materialsmentioning

confidence: 99%

Recent Advances in Topical Hemostatic Materials

Liu,

Zhang,

Yao

et al. 2024

ACS Appl. Bio Mater.

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Untimely or improper treatment of traumatic bleeding may cause secondary injuries and even death. The traditional hemostatic modes can no longer meet requirements of coping with complicated bleeding emergencies. With scientific and technological advancements, a variety of topical hemostatic materials have been investigated involving inorganic, biological, polysaccharide, and carbonbased hemostatic materials. These materials have their respective merits and defects. In this work, the application and mechanism of the major hemostatic materials, especially some hemostatic nanomaterials with excellent adhesion, good biocompatibility, low toxicity, and high adsorption capacity, are summarized. In the future, it is the prospect to develop multifunctional hemostatic materials with hemostasis and antibacterial and anti-inflammatory properties for promoting wound healing.

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“…In Vitro Blood Clotting Experiment. Blood clotting experiments were conducted according to the method presented by Qiao et al 41,42 The SPG film was cut into 1.0 cm × 1.0 cm and was placed in the polypropylene dish, with a prewarmed temperature of 37 °C for 5 min. Experimental blood was collected aseptically from the hearts of Kunming mice using sodium citrate as an anticoagulant.…”

Section: Tensile Test Of Filmsmentioning

confidence: 99%

Bioinspired Natural Shellac Dressing for Rapid Wound Sealing and Healing

Liu,

Li,

Dong

et al. 2023

ACS Appl. Mater. Interfaces

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Developing safe and effective wound dressings that address the complexities of wound healing is an ongoing goal in biomaterials research. Inspired by the shield used to protect lac insects, we have designed and developed a type of bioactive shellacbased wound dressing in this paper. The dressing exhibited a high adhesion energy of 146.6 J•m −2 in porcine skin and showed a reversible binding due to its pH sensitivity. Meanwhile, a novel "shellac-like" compound, n-octacosanol gallate ester, has been synthesized and added to the dressing to improve its antibacterial and blood coagulation properties. The novel shellac-based dressing could be sprayed to form a sticky film within 70 s for rapid hemostasis and wound sealing, which could be conveniently applied to various wounds on extensible body parts. In addition, the shellac-based dressing can actively promote the healing of a full-thickness wound in the skin of mice. We also used molecular dynamics simulations to investigate the interactions between the shellac molecule and the phospholipid bilayer and attempted to show that the shellac molecule was beneficial for wound healing. This work provides a novel and practical bioinspired wound dressing with significant properties, facile preparation, and ease of use, which is an interesting alternative to its traditional counterparts.

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Gelatin/calcium chloride electrospun nanofibers for rapid hemostasis

Abstract: Blood coagulation is the body’s main defense to bleeding caused by trauma and is divided into endogenous and exogenous pathways. Calcium ions play a very important role in the process...

Cited by 32 publications

References 52 publications

One-step Preparation of Antibacterial Gelatin/Polycaprolactone Nanofibrous Janus Membranes for Efficient Hemostasis

One-step Preparation of Antibacterial Gelatin/Polycaprolactone Nanofibrous Janus Membranes for Efficient Hemostasis

Recent Advances in Topical Hemostatic Materials

Bioinspired Natural Shellac Dressing for Rapid Wound Sealing and Healing

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