Gelatin/carboxymethyl cellulose mucoadhesive films with lysozyme: Development and characterization

Abstract

The goal of our study is to develop and characterize mucoadhesive films with entrapped lysozyme based on gelatin/sodium carboxymethyl cellulose as perspective antimicrobial preparation. Lysozyme in mucoadhesive films retains more than 95 % of its initial activity for 3 years of storage. Different physical-chemical and biochemical characteristics of entrapped enzyme were evaluated, such as film thickness, weight, time of dissolution in water, bioadhesive force, in vitro lysozyme release, pH- and thermoprofiles of hydrolytic activity, effect of γ-sterilization, etc. We have shown that gelatin/sodium carboxymethyl cellulose films have adhesive force on the level of 4380 Pa. Scanning electron microscopy images shows the relative uniformity of the gelatin surface with entrapped lysozyme. Mucoadhesive films with lysozyme have 100% bactericidal effect on the test strain, Staphylococcus aureus ATCC 25923 F - 49 and thus could be considered as a perspective antimicrobial preparation.

Preparation of a balsa-lysozyme eco-friendly dressing and its effect on wound healing

“…Because lysozyme is embedded in balsa structure by glue method. 8,11 The encapsulation structure is poor in density, and lysozyme has good water solubility. The lysozyme-balsa was cultured in PBS buffer solution and the lysozyme was easily released to PBS through the balsa surface structure, resulting in an increase in release.…”

“…9 In recent years, there have been many studies to prepare lysozyme into microcapsules, nanoparticles, and pellets, and combine with different materials to improve its stability and its Gram-negative bacteria bactericidal effect. 7,10,11 Some studies have pointed out the hydrolysis activity of PTFE membrane loaded with lysozyme against the micrococcus lysate can reach 3.5 U cm À2 , which has perfect antibacterial activity against the important pathogenic Staphylococcus aureus, and the antibacterial rate reaches 89%. The antibacterial rate is expected to be further improved if the rate of enzyme load per unit area is increased.…”

“…Clinically, it is oen used in the form of a microballoon or in combination with other antimicrobial agents. 10,11 For patients with severe skin defects, there is an urgent need to quickly and effectively close the wound. Effective and inexpensive strategies to prevent bacterial invasion and the loss of body uids are needed.…”

Synergism Effect of Surfactant and Inorganic Salt on the Properties of Starch/Poly(Vinyl Alcohol) Film

“…The MC of starch/PVA film at RH of 33, 54, and 76% were measured and recorded in Table 3. From Table 3 it could be 2 the MC of starch/PVA was even lower than that of pure starch/PVA at the same RH. The increasing water resisting property of starch/PVA film in humidity environment would be benefit for its application.…”

Features of the Hydrolysis Kinetics of Micrococcus lysodeikticus by Immobilized Lysozyme

“…Раніше нами були розроблені полімерні системи антибактеріальної дії з іммобілізованим лізоцимом у вигляді гелів [5], гідрогелевих покриттів [6] і плівок [7]. Використання для іммобілізації ензиму мукоадгезивних полімерів, а саме натрієвої солі карбоксиметилцелюлози, полівінілового спирту і желатину, сприяли закріпленню препаратів на слизових тканинах і про-яву ензимом максимальної гідролітичної активності, збереженню антимікробних властивостей, пролонгованості терапевтичної дії та високій стабільності.…”